MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway
Objectives The objective was to investigate the effects of microRNA-421 against myocardial ischemia/reperfusion injury in C57BL/6 mice. Methods Male C57BL/6 mice (n = 27) were randomly divided into three groups: normal control (NC) group (sham-treated); I/R model group, which underwent the I 30min /...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-10-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/0300060519871863 |
| _version_ | 1818966349302464512 |
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| author | Lin-lin Guo Ming-lei Guo Jian Yao Yun-qi Weng Xue-zhi Zhang |
| author_facet | Lin-lin Guo Ming-lei Guo Jian Yao Yun-qi Weng Xue-zhi Zhang |
| author_sort | Lin-lin Guo |
| collection | DOAJ |
| description | Objectives The objective was to investigate the effects of microRNA-421 against myocardial ischemia/reperfusion injury in C57BL/6 mice. Methods Male C57BL/6 mice (n = 27) were randomly divided into three groups: normal control (NC) group (sham-treated); I/R model group, which underwent the I 30min /R 24h model (ischemia for 30 minutes followed by reperfusion for 24 hours); and the miRNA group, which were injected with miR-421. Pathology was assessed by hematoxylin and eosin staining and myocardial infarct size was measured by triphenyltetrazolium chloride staining. The apoptosis rate was measured by TUNEL assay, and relative expression of toll-like receptor-4 (TLR4), Janus kinase 2 (JAK2), and signal transducer and activator of translation 3 (STAT3) was evaluated by immunohistochemistry. Interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-10, and high mobility group protein B1 (HMGB1) serum concentrations were measured by ELISA. Results Compared with the NC group, in the model group, the myocardial infarction was large; inflammatory cell infiltration was severe; apoptosis was enhanced; expression of TLR4, JAK2, and STAT3 was increased; and serum concentrations of IL-6, TNF-α, IL-10, and HMGB1 were significantly increased. In the miRNA group, the ischemia/reperfusion injury was significantly improved. Conclusions Overexpression of miRNA-421 could reduce ischemia/reperfusion inflammatory response, perhaps via inactivation of TLR4, JAK2, and STAT3. |
| first_indexed | 2024-12-20T13:31:29Z |
| format | Article |
| id | doaj.art-7a13fbb6d9c949f18adfdbd3a8c4010c |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-12-20T13:31:29Z |
| publishDate | 2020-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-7a13fbb6d9c949f18adfdbd3a8c4010c2022-12-21T19:39:05ZengSAGE PublishingJournal of International Medical Research1473-23002020-10-014810.1177/0300060519871863MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathwayLin-lin GuoMing-lei GuoJian YaoYun-qi WengXue-zhi ZhangObjectives The objective was to investigate the effects of microRNA-421 against myocardial ischemia/reperfusion injury in C57BL/6 mice. Methods Male C57BL/6 mice (n = 27) were randomly divided into three groups: normal control (NC) group (sham-treated); I/R model group, which underwent the I 30min /R 24h model (ischemia for 30 minutes followed by reperfusion for 24 hours); and the miRNA group, which were injected with miR-421. Pathology was assessed by hematoxylin and eosin staining and myocardial infarct size was measured by triphenyltetrazolium chloride staining. The apoptosis rate was measured by TUNEL assay, and relative expression of toll-like receptor-4 (TLR4), Janus kinase 2 (JAK2), and signal transducer and activator of translation 3 (STAT3) was evaluated by immunohistochemistry. Interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-10, and high mobility group protein B1 (HMGB1) serum concentrations were measured by ELISA. Results Compared with the NC group, in the model group, the myocardial infarction was large; inflammatory cell infiltration was severe; apoptosis was enhanced; expression of TLR4, JAK2, and STAT3 was increased; and serum concentrations of IL-6, TNF-α, IL-10, and HMGB1 were significantly increased. In the miRNA group, the ischemia/reperfusion injury was significantly improved. Conclusions Overexpression of miRNA-421 could reduce ischemia/reperfusion inflammatory response, perhaps via inactivation of TLR4, JAK2, and STAT3.https://doi.org/10.1177/0300060519871863 |
| spellingShingle | Lin-lin Guo Ming-lei Guo Jian Yao Yun-qi Weng Xue-zhi Zhang MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway Journal of International Medical Research |
| title | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway |
| title_full | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway |
| title_fullStr | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway |
| title_full_unstemmed | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway |
| title_short | MicroRNA-421 improves ischemia/reperfusion injury via regulation toll-like receptor 4 pathway |
| title_sort | microrna 421 improves ischemia reperfusion injury via regulation toll like receptor 4 pathway |
| url | https://doi.org/10.1177/0300060519871863 |
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