Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome
Abiotic stresses are considered the most deleterious factor affecting growth and development of plants worldwide. Such stresses are largely unavoidable and trigger adaptive responses affecting different cellular processes and target different compartments. Shotgun proteomic and mass spectrometry-bas...
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| Format: | Article |
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MDPI AG
2018-10-01
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| Series: | Proteomes |
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| Online Access: | http://www.mdpi.com/2227-7382/6/4/38 |
| _version_ | 1798034189498449920 |
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| author | May Alqurashi Marco Chiapello Chantal Bianchet Francesco Paolocci Kathryn S. Lilley Christoph Gehring |
| author_facet | May Alqurashi Marco Chiapello Chantal Bianchet Francesco Paolocci Kathryn S. Lilley Christoph Gehring |
| author_sort | May Alqurashi |
| collection | DOAJ |
| description | Abiotic stresses are considered the most deleterious factor affecting growth and development of plants worldwide. Such stresses are largely unavoidable and trigger adaptive responses affecting different cellular processes and target different compartments. Shotgun proteomic and mass spectrometry-based approaches offer an opportunity to elucidate the response of the proteome to abiotic stresses. In this study, the severe drought or water-deficit response in Arabidopsis thaliana was mimicked by treating cell suspension callus with 40% polyethylene glycol for 10 and 30 min. Resulting data demonstrated that 310 proteins were differentially expressed in response to this treatment with a strict ±2.0-fold change. Over-representation was observed in the gene ontology categories of ‘ribosome’ and its related functions as well as ‘oxidative phosphorylation’, indicating both structural and functional drought responses at the cellular level. Proteins in the category ‘endocytosis’ also show significant enrichment and this is consistent with increased active transport and recycling of membrane proteins in response to abiotic stress. This is supported by the particularly pronounced enrichment in proteins of the endosomal sorting complexes that are required for membrane remodelling. Taken together, the findings point to rapid and complex physiological and structural changes essential for survival in response to sudden severe drought stress. |
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| format | Article |
| id | doaj.art-7a16447b529b49a3bc04712e48a4afdb |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-11T20:40:44Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
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| series | Proteomes |
| spelling | doaj.art-7a16447b529b49a3bc04712e48a4afdb2022-12-22T04:04:13ZengMDPI AGProteomes2227-73822018-10-01643810.3390/proteomes6040038proteomes6040038Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture ProteomeMay Alqurashi0Marco Chiapello1Chantal Bianchet2Francesco Paolocci3Kathryn S. Lilley4Christoph Gehring5Department of Biochemistry, Cambridge Centre for Proteomics, Cambridge System Biology Centre, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UKDepartment of Biochemistry, Cambridge Centre for Proteomics, Cambridge System Biology Centre, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UKDepartment of Chemistry, Biology & Biotechnology, Borgo XX giugno 74, 06121 Perugia, ItalyCNR, Institute of Biosciences and Bioresources, Perugia Division, Via Madonna Alta, 130 06128 Perugia, ItalyDepartment of Biochemistry, Cambridge Centre for Proteomics, Cambridge System Biology Centre, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UKBiological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi ArabiaAbiotic stresses are considered the most deleterious factor affecting growth and development of plants worldwide. Such stresses are largely unavoidable and trigger adaptive responses affecting different cellular processes and target different compartments. Shotgun proteomic and mass spectrometry-based approaches offer an opportunity to elucidate the response of the proteome to abiotic stresses. In this study, the severe drought or water-deficit response in Arabidopsis thaliana was mimicked by treating cell suspension callus with 40% polyethylene glycol for 10 and 30 min. Resulting data demonstrated that 310 proteins were differentially expressed in response to this treatment with a strict ±2.0-fold change. Over-representation was observed in the gene ontology categories of ‘ribosome’ and its related functions as well as ‘oxidative phosphorylation’, indicating both structural and functional drought responses at the cellular level. Proteins in the category ‘endocytosis’ also show significant enrichment and this is consistent with increased active transport and recycling of membrane proteins in response to abiotic stress. This is supported by the particularly pronounced enrichment in proteins of the endosomal sorting complexes that are required for membrane remodelling. Taken together, the findings point to rapid and complex physiological and structural changes essential for survival in response to sudden severe drought stress.http://www.mdpi.com/2227-7382/6/4/38abiotic stressdroughtribosomeoxidative phosphorylationendosomal sorting complex |
| spellingShingle | May Alqurashi Marco Chiapello Chantal Bianchet Francesco Paolocci Kathryn S. Lilley Christoph Gehring Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome Proteomes abiotic stress drought ribosome oxidative phosphorylation endosomal sorting complex |
| title | Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome |
| title_full | Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome |
| title_fullStr | Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome |
| title_full_unstemmed | Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome |
| title_short | Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome |
| title_sort | early responses to severe drought stress in the arabidopsis thaliana cell suspension culture proteome |
| topic | abiotic stress drought ribosome oxidative phosphorylation endosomal sorting complex |
| url | http://www.mdpi.com/2227-7382/6/4/38 |
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