Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat models
Extra cellular matrix (ECM) incorporated calcium hydroxyapatite bound with triphasic silica is a custom made bone graft with hybrid properties which is used for bone regeneration. A promising new technique for preparing natural matrices for bone regeneration now in trend is decellularisation. Accord...
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Director of Academics and Research, Kerala Veterinary and Animal Sciences University
2023-12-01
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| Series: | Journal of Veterinary and Animal Sciences |
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| Online Access: | https://jvas.in/public_html/upload/article_file/article_file_s6kiht.pdf?t=s6kiht |
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| author | M. Manasa P.T. Dinesh F.B. Fernandez S. Sooryadas Hamza Palekkodan S. Anoop N. S. Jineshkumar V. Remya H.K. Varma |
| author_facet | M. Manasa P.T. Dinesh F.B. Fernandez S. Sooryadas Hamza Palekkodan S. Anoop N. S. Jineshkumar V. Remya H.K. Varma |
| author_sort | M. Manasa |
| collection | DOAJ |
| description | Extra cellular matrix (ECM) incorporated calcium hydroxyapatite bound with triphasic silica is a custom made bone graft with hybrid properties which is used for bone regeneration. A promising new technique for preparing natural matrices for bone regeneration now in trend is decellularisation. According to reports, the ECM activates mesenchymal stem cells, speeding up bone healing. Although research employing hard tissues like bone are scarce, successful decellularisation has been done using a variety of tissues including skin, cartilage and tendon. The study was carried out in rat calvaria; critical size defects were created and later grafted with decellularised tissue engineered HASi bioceramic (test) and plain HASi bioceramic (control) on left and right side respectively. Radiography was used to assess changes in the graft, the rate of its degradation and the degree of bone repair. Postoperative dorsoventral view of the skull was taken immediately after surgery and thereafter on 2nd, 4th, 8th and 12th week post-surgery. The grafts were found to remain stable throughout the healing period. By 4th week onwards signs of gradual degradation of material with regeneration of bone could be noticed especially on test side compared to control. Test material was found to be completely integrated with host bone by eight weeks whereas control graft took 12 weeks. At twelve weeks, both the test and control grafts showed complete integration with host bone. It was concluded that the test graft material successfully hastened bone regeneration, making it a promising alternative to conventional bone grafts. |
| first_indexed | 2024-03-08T14:31:04Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 0971-0701 2582-0605 |
| language | English |
| last_indexed | 2024-03-08T14:31:04Z |
| publishDate | 2023-12-01 |
| publisher | Director of Academics and Research, Kerala Veterinary and Animal Sciences University |
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| series | Journal of Veterinary and Animal Sciences |
| spelling | doaj.art-7a176b24281848cb857b65682f300a5f2024-01-12T09:58:21ZengDirector of Academics and Research, Kerala Veterinary and Animal Sciences UniversityJournal of Veterinary and Animal Sciences0971-07012582-06052023-12-0154491692010.51966/jvas.2023.54.4.916-920Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat modelsM. Manasa0P.T. Dinesh1https://orcid.org/0000-0002-3529-7364F.B. Fernandez2S. Sooryadas3https://orcid.org/0000-0002-4433-8025Hamza Palekkodan4https://orcid.org/0000-0002-5278-0147S. Anoop5https://orcid.org/0000-0002-0054-6164N. S. Jineshkumar6https://orcid.org/0009-0009-0860-6449V. Remya7https://orcid.org/0009-0003-1345-9734H.K. Varma8MVSc Scholar, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, IndiaAssistant Professor, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, IndiaScientist C, Division of Bioceramics, BMT wing, SCTIMST ThiruvananthapuramAssistant Professor, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, IndiaAssistant professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Kerala, IndiaProfessor and head, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, IndiaAssistant Professor, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, IndiaAssistant Professor, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, IndiaHead BMT wing, SCTIMST ThiruvananthapuramExtra cellular matrix (ECM) incorporated calcium hydroxyapatite bound with triphasic silica is a custom made bone graft with hybrid properties which is used for bone regeneration. A promising new technique for preparing natural matrices for bone regeneration now in trend is decellularisation. According to reports, the ECM activates mesenchymal stem cells, speeding up bone healing. Although research employing hard tissues like bone are scarce, successful decellularisation has been done using a variety of tissues including skin, cartilage and tendon. The study was carried out in rat calvaria; critical size defects were created and later grafted with decellularised tissue engineered HASi bioceramic (test) and plain HASi bioceramic (control) on left and right side respectively. Radiography was used to assess changes in the graft, the rate of its degradation and the degree of bone repair. Postoperative dorsoventral view of the skull was taken immediately after surgery and thereafter on 2nd, 4th, 8th and 12th week post-surgery. The grafts were found to remain stable throughout the healing period. By 4th week onwards signs of gradual degradation of material with regeneration of bone could be noticed especially on test side compared to control. Test material was found to be completely integrated with host bone by eight weeks whereas control graft took 12 weeks. At twelve weeks, both the test and control grafts showed complete integration with host bone. It was concluded that the test graft material successfully hastened bone regeneration, making it a promising alternative to conventional bone grafts.https://jvas.in/public_html/upload/article_file/article_file_s6kiht.pdf?t=s6kihtdecellularised tissue engineered hasi bioceramiccritical size calvarial defectextra cellular matrix |
| spellingShingle | M. Manasa P.T. Dinesh F.B. Fernandez S. Sooryadas Hamza Palekkodan S. Anoop N. S. Jineshkumar V. Remya H.K. Varma Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat models Journal of Veterinary and Animal Sciences decellularised tissue engineered hasi bioceramic critical size calvarial defect extra cellular matrix |
| title | Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat models |
| title_full | Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat models |
| title_fullStr | Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat models |
| title_full_unstemmed | Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat models |
| title_short | Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat models |
| title_sort | radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered has i in rat models |
| topic | decellularised tissue engineered hasi bioceramic critical size calvarial defect extra cellular matrix |
| url | https://jvas.in/public_html/upload/article_file/article_file_s6kiht.pdf?t=s6kiht |
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