Toll-Like Receptor 2 (TLR-2) Gene Polymorphisms In Type 2 Diabetes Mellitus
Objective Innate immunity factors are associated with type 2 diabetes (T2DM) and its complications. Therefore, T2DM has been suggested to be an immune-dependent disease. Elevated fasting glucose level and higher concentrations of innate immunity soluble molecules are not only related with insulin r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2018-08-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://celljournal.org/journal/article/abstract/5540 |
| _version_ | 1828861098361618432 |
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| author | Zeynep Ermiş Karaali Gonca Candan Mehmet Burak Aktuğlu Mustafa Velet Arzu Ergen |
| author_facet | Zeynep Ermiş Karaali Gonca Candan Mehmet Burak Aktuğlu Mustafa Velet Arzu Ergen |
| author_sort | Zeynep Ermiş Karaali |
| collection | DOAJ |
| description | Objective
Innate immunity factors are associated with type 2 diabetes (T2DM) and its complications. Therefore, T2DM has been suggested to be an immune-dependent disease. Elevated fasting glucose level and higher concentrations of innate immunity soluble molecules are not only related with insulin resistance, but inflammation is also an important factor in beta cell dysfunction in T2DM. Toll-like receptor 2 (TLR-2), which has an important role in inducing innate immune cells, is thought to have suppressive roles on immune responses in T2DM. We therefore aimed to investigate the possible role of TLR-2 del -196-174 and Arg753Gln variants in T2DM pathogenesis.
Materials And Methods
This study was designed as a case-control study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotype the two variants in 100 T2DM patients and 98 age- matched controls.
Results
We found significantly higher frequencies of TLR-2 del -196-174 DD genotype (P=0.003), ID genotype (P=0.009) and D allele (P=0.001) in patients compared with controls. In addition, the II genotype (P=0.001) and the I allele (P=0.003) frequencies were elevated in healthy controls. We did not find any significant differences in frequency distribution for the Arg753Gln variant in study groups.
Conclusion
We suggest that carrying the D allele of the TLR-2 del -196-174 variant may be related as a risk factor for T2DM. |
| first_indexed | 2024-12-13T02:53:52Z |
| format | Article |
| id | doaj.art-7a17c712b2234da68f8dbfe3758a2d3c |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-13T02:53:52Z |
| publishDate | 2018-08-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-7a17c712b2234da68f8dbfe3758a2d3c2022-12-22T00:01:59ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142018-08-0120455956310.22074/cellj.2019.5540Toll-Like Receptor 2 (TLR-2) Gene Polymorphisms In Type 2 Diabetes MellitusZeynep Ermiş Karaali0Gonca Candan1Mehmet Burak Aktuğlu2Mustafa Velet3Arzu Ergen4Department of Internal Medicine, Haseki Training and Research Hospital, University of Health Sciences, Istanbul, TurkeyDepartment of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, TurkeyDepartment of Internal Medicine, Haseki Training and Research Hospital, University of Health Sciences, Istanbul, TurkeyDepartment of Internal Medicine, Haseki Training and Research Hospital, University of Health Sciences, Istanbul, TurkeyDepartment of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, TurkeyObjective Innate immunity factors are associated with type 2 diabetes (T2DM) and its complications. Therefore, T2DM has been suggested to be an immune-dependent disease. Elevated fasting glucose level and higher concentrations of innate immunity soluble molecules are not only related with insulin resistance, but inflammation is also an important factor in beta cell dysfunction in T2DM. Toll-like receptor 2 (TLR-2), which has an important role in inducing innate immune cells, is thought to have suppressive roles on immune responses in T2DM. We therefore aimed to investigate the possible role of TLR-2 del -196-174 and Arg753Gln variants in T2DM pathogenesis. Materials And Methods This study was designed as a case-control study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotype the two variants in 100 T2DM patients and 98 age- matched controls. Results We found significantly higher frequencies of TLR-2 del -196-174 DD genotype (P=0.003), ID genotype (P=0.009) and D allele (P=0.001) in patients compared with controls. In addition, the II genotype (P=0.001) and the I allele (P=0.003) frequencies were elevated in healthy controls. We did not find any significant differences in frequency distribution for the Arg753Gln variant in study groups. Conclusion We suggest that carrying the D allele of the TLR-2 del -196-174 variant may be related as a risk factor for T2DM.https://celljournal.org/journal/article/abstract/5540DiabetesInflammationPolymorphismTLRs |
| spellingShingle | Zeynep Ermiş Karaali Gonca Candan Mehmet Burak Aktuğlu Mustafa Velet Arzu Ergen Toll-Like Receptor 2 (TLR-2) Gene Polymorphisms In Type 2 Diabetes Mellitus Cell Journal Diabetes Inflammation Polymorphism TLRs |
| title | Toll-Like Receptor 2 (TLR-2) Gene Polymorphisms In Type 2 Diabetes Mellitus |
| title_full | Toll-Like Receptor 2 (TLR-2) Gene Polymorphisms In Type 2 Diabetes Mellitus |
| title_fullStr | Toll-Like Receptor 2 (TLR-2) Gene Polymorphisms In Type 2 Diabetes Mellitus |
| title_full_unstemmed | Toll-Like Receptor 2 (TLR-2) Gene Polymorphisms In Type 2 Diabetes Mellitus |
| title_short | Toll-Like Receptor 2 (TLR-2) Gene Polymorphisms In Type 2 Diabetes Mellitus |
| title_sort | toll like receptor 2 tlr 2 gene polymorphisms in type 2 diabetes mellitus |
| topic | Diabetes Inflammation Polymorphism TLRs |
| url | https://celljournal.org/journal/article/abstract/5540 |
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