Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods
The objective of this study was to analyze the antimicrobial effect of 2% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) by agar diffusion test and by direct exposure test. Five microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Bacillus subtilis, Candida a...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Odontológica de Ribeirão Preto
2003-01-01
|
| Series: | Brazilian Dental Journal |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000100011 |
| _version_ | 1818574733902348288 |
|---|---|
| author | Estrela Carlos Ribeiro Rosane Galhardo Estrela Cyntia R.A. Pécora Jesus Djalma Sousa-Neto Manoel Damião |
| author_facet | Estrela Carlos Ribeiro Rosane Galhardo Estrela Cyntia R.A. Pécora Jesus Djalma Sousa-Neto Manoel Damião |
| author_sort | Estrela Carlos |
| collection | DOAJ |
| description | The objective of this study was to analyze the antimicrobial effect of 2% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) by agar diffusion test and by direct exposure test. Five microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and one mixture of these were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37ºC for 24 h. For the agar diffusion test (ADT), 18 Petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth in junction. Fifty-four paper disks (9 mm in diameter) were immersed in the experimental solutions for 1 min. Subsequently, three papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were maintained for 1 h at room temperature, and then incubated at 37ºC for 48 h. The diameter of microbial inhibition was measured around the papers disks containing the substances. For the direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and were then placed on Petri plates and covered with one of the irrigant solutions, or with sterile distilled water (control group). After intervals of 5, 10 and 30 min, the paper points were removed from contact with the solutions and individually immersed in 7 ml of Letheen Broth, followed by incubation at 37ºC for 48 h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37ºC for 48 h. Bacterial growth was again evaluated by turbidity of the culture medium. Gram stain of BHI cultures was used for verification of contamination and growth was determined by macroscopic and microscopic examination. The best performance of antimicrobial effectiveness of NaOCl was observed in the direct exposure test, and of CHX was observed in the agar diffusion test. The magnitude of antimicrobial effect was influenced by the experimental methods, biological indicators and exposure time. |
| first_indexed | 2024-12-15T00:30:28Z |
| format | Article |
| id | doaj.art-7a18d696cd074fa29369715808dc9778 |
| institution | Directory Open Access Journal |
| issn | 0103-6440 |
| language | English |
| last_indexed | 2024-12-15T00:30:28Z |
| publishDate | 2003-01-01 |
| publisher | Fundação Odontológica de Ribeirão Preto |
| record_format | Article |
| series | Brazilian Dental Journal |
| spelling | doaj.art-7a18d696cd074fa29369715808dc97782022-12-21T22:42:02ZengFundação Odontológica de Ribeirão PretoBrazilian Dental Journal0103-64402003-01-011415862Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methodsEstrela CarlosRibeiro Rosane GalhardoEstrela Cyntia R.A.Pécora Jesus DjalmaSousa-Neto Manoel DamiãoThe objective of this study was to analyze the antimicrobial effect of 2% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) by agar diffusion test and by direct exposure test. Five microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and one mixture of these were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37ºC for 24 h. For the agar diffusion test (ADT), 18 Petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth in junction. Fifty-four paper disks (9 mm in diameter) were immersed in the experimental solutions for 1 min. Subsequently, three papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were maintained for 1 h at room temperature, and then incubated at 37ºC for 48 h. The diameter of microbial inhibition was measured around the papers disks containing the substances. For the direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and were then placed on Petri plates and covered with one of the irrigant solutions, or with sterile distilled water (control group). After intervals of 5, 10 and 30 min, the paper points were removed from contact with the solutions and individually immersed in 7 ml of Letheen Broth, followed by incubation at 37ºC for 48 h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37ºC for 48 h. Bacterial growth was again evaluated by turbidity of the culture medium. Gram stain of BHI cultures was used for verification of contamination and growth was determined by macroscopic and microscopic examination. The best performance of antimicrobial effectiveness of NaOCl was observed in the direct exposure test, and of CHX was observed in the agar diffusion test. The magnitude of antimicrobial effect was influenced by the experimental methods, biological indicators and exposure time.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000100011sodium hypochloritechlorhexidineirrigant solutionsintracanal dressing |
| spellingShingle | Estrela Carlos Ribeiro Rosane Galhardo Estrela Cyntia R.A. Pécora Jesus Djalma Sousa-Neto Manoel Damião Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods Brazilian Dental Journal sodium hypochlorite chlorhexidine irrigant solutions intracanal dressing |
| title | Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods |
| title_full | Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods |
| title_fullStr | Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods |
| title_full_unstemmed | Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods |
| title_short | Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods |
| title_sort | antimicrobial effect of 2 sodium hypochlorite and 2 chlorhexidine tested by different methods |
| topic | sodium hypochlorite chlorhexidine irrigant solutions intracanal dressing |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000100011 |
| work_keys_str_mv | AT estrelacarlos antimicrobialeffectof2sodiumhypochloriteand2chlorhexidinetestedbydifferentmethods AT ribeirorosanegalhardo antimicrobialeffectof2sodiumhypochloriteand2chlorhexidinetestedbydifferentmethods AT estrelacyntiara antimicrobialeffectof2sodiumhypochloriteand2chlorhexidinetestedbydifferentmethods AT pecorajesusdjalma antimicrobialeffectof2sodiumhypochloriteand2chlorhexidinetestedbydifferentmethods AT sousanetomanoeldamiao antimicrobialeffectof2sodiumhypochloriteand2chlorhexidinetestedbydifferentmethods |