The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research.
Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0233057 |
| _version_ | 1818833843156680704 |
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| author | Seongjun So Yeonmi Lee Jiwan Choi Seoon Kang Ji-Yoon Lee Julie Hwang Joosung Shin James R Dutton Eul-Ju Seo Beom Hee Lee Chong Jai Kim Shoukhrat Mitalipov Soo Jin Oh Eunju Kang |
| author_facet | Seongjun So Yeonmi Lee Jiwan Choi Seoon Kang Ji-Yoon Lee Julie Hwang Joosung Shin James R Dutton Eul-Ju Seo Beom Hee Lee Chong Jai Kim Shoukhrat Mitalipov Soo Jin Oh Eunju Kang |
| author_sort | Seongjun So |
| collection | DOAJ |
| description | Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27632, and did not affect pluripotency, differentiation ability, and chromosome integrity. Furthermore, fasudil promoted retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells (NCCs) during differentiation. It was also useful for single-cell passaging of hPSCs and during aggregation. These findings suggest that fasudil can replace Y-27632 for use in stem research. |
| first_indexed | 2024-12-19T02:25:22Z |
| format | Article |
| id | doaj.art-7a20225a742d4ddd8bcbbb45190f4ce2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T02:25:22Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7a20225a742d4ddd8bcbbb45190f4ce22022-12-21T20:39:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01155e023305710.1371/journal.pone.0233057The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research.Seongjun SoYeonmi LeeJiwan ChoiSeoon KangJi-Yoon LeeJulie HwangJoosung ShinJames R DuttonEul-Ju SeoBeom Hee LeeChong Jai KimShoukhrat MitalipovSoo Jin OhEunju KangPoor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27632, and did not affect pluripotency, differentiation ability, and chromosome integrity. Furthermore, fasudil promoted retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells (NCCs) during differentiation. It was also useful for single-cell passaging of hPSCs and during aggregation. These findings suggest that fasudil can replace Y-27632 for use in stem research.https://doi.org/10.1371/journal.pone.0233057 |
| spellingShingle | Seongjun So Yeonmi Lee Jiwan Choi Seoon Kang Ji-Yoon Lee Julie Hwang Joosung Shin James R Dutton Eul-Ju Seo Beom Hee Lee Chong Jai Kim Shoukhrat Mitalipov Soo Jin Oh Eunju Kang The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research. PLoS ONE |
| title | The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research. |
| title_full | The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research. |
| title_fullStr | The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research. |
| title_full_unstemmed | The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research. |
| title_short | The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research. |
| title_sort | rho associated kinase inhibitor fasudil can replace y 27632 for use in human pluripotent stem cell research |
| url | https://doi.org/10.1371/journal.pone.0233057 |
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