The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research.

The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research.

Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used...

Full description

Bibliographic Details
Main Authors:	Seongjun So, Yeonmi Lee, Jiwan Choi, Seoon Kang, Ji-Yoon Lee, Julie Hwang, Joosung Shin, James R Dutton, Eul-Ju Seo, Beom Hee Lee, Chong Jai Kim, Shoukhrat Mitalipov, Soo Jin Oh, Eunju Kang
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2020-01-01
Series:	PLoS ONE
Online Access:	https://doi.org/10.1371/journal.pone.0233057

Similar Items

Fasudil Increases the Establishment of Somatic Cell Nuclear Transfer Embryonic Stem Cells in Mouse
by: Seongjun So, et al.
Published: (2020-03-01)

The Rho-associated kinase inhibitors Y27632 and fasudil promote microglial migration in the spinal cord via the ERK signaling pathway
by: Pei-Cai Fu, et al.
Published: (2018-01-01)

Efficient method for generating homozygous embryonic stem cells in mice
by: Bitnara Kim, et al.
Published: (2022-03-01)

Mitochondrial DNA Haplogroup Related to the Prevalence of <i>Helicobacter pylori</i>
by: Yeonmi Lee, et al.
Published: (2021-09-01)

The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia
by: Ya-yun Yan, et al.
Published: (2015-01-01)

Advantages of Rho-associated kinases and their inhibitor fasudil for the treatment of neurodegenerative diseases
by: Qing Wang, et al.
Published: (2022-01-01)

Influence of Rho/ROCK inhibitor Y-27632 on proliferation of equine mesenchymal stromal cells
by: Michaela Melzer, et al.
Published: (2023-06-01)

Artificial Oocyte: Development and Potential Application
by: Reza K. Oqani, et al.
Published: (2022-03-01)

Efficient hepatic differentiation and regeneration potential under xeno-free conditions using mass-producible amnion-derived mesenchymal stem cells
by: Jiwan Choi, et al.
Published: (2021-11-01)

Haploidy of somatic cells in mouse oocyte using somatic cell nuclear transfer
by: Yeonmi Lee, et al.
Published: (2022-12-01)

Hormone induced recipients for embryo transfer in mice
by: Yeonmi Lee, et al.
Published: (2021-12-01)

Inhibition of RhoA/Rho kinase signaling pathway by fasudil protects against kainic acid‐induced neurite injury
by: Yingchun Xiang, et al.
Published: (2021-08-01)

Inhibition of rho-kinase by fasudil suppresses formation and progression of experimental abdominal aortic aneurysms.
by: Chen Peng, et al.
Published: (2013-01-01)

Inhibition of Agonist-Induced Positive Inotropy by a Selective Rho-Associated Kinase Inhibitor, Y-27632
by: Kazuhide Nishimaru, et al.
Published: (2003-01-01)

De Novo Development of mtDNA Deletion Due to Decreased POLG and SSBP1 Expression in Humans
by: Yeonmi Lee, et al.
Published: (2021-02-01)

Retraction: Inhibition of Rho-kinase by fasudil suppresses formation and progression of experimental abdominal aortic aneurysms.
Published: (2014-01-01)

Effects of Rho-Associated Kinase (Rock) Inhibitors (Alternative to Y-27632) on Primary Human Corneal Endothelial Cells
by: Gary S. L. Peh, et al.
Published: (2023-05-01)

Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632.
by: Erik D Anderson, et al.
Published: (2018-01-01)

Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells
by: Stark G Björn, et al.
Published: (2008-09-01)

Rho-Associated Kinase Inhibitor (Y-27632) Attenuates Doxorubicin-Induced Apoptosis of Human Cardiac Stem Cells.
by: Lijuan Kan, et al.
Published: (2015-01-01)

Hepatogenic Potential and Liver Regeneration Effect of Human Liver-derived Mesenchymal-Like Stem Cells
by: Jooyoung Lee, et al.
Published: (2020-06-01)

Stem cell therapy for dogs with immune-mediated hemolytic anemia
by: Yeonmi Lee, et al.
Published: (2024-03-01)

The rho kinase inhibitor Y-27632 improves motor performance in male SOD1-G93A mice
by: Rene eGünther, et al.
Published: (2014-10-01)

A Rho Kinase (ROCK) Inhibitor, Y-27632, Inhibits the Dissociation-Induced Cell Death of Salivary Gland Stem Cells
by: Kichul Kim, et al.
Published: (2021-05-01)

Comparative investigation of antitumoral effectiveness of Rho-kinase inhibitor Y-27632, pravastatin and atorvastatin in anaplastic thyroid cancer cell culture
by: Erdinc Nayir, et al.
Published: (2017-07-01)

Germline and somatic mtDNA mutations in mouse aging.
by: Hong Ma, et al.
Published: (2018-01-01)

The non-selective Rho-kinase inhibitors Y-27632 and Y-33075 decrease contraction but increase migration in murine and human hepatic stellate cells.
by: Nadine Bachtler, et al.
Published: (2023-01-01)

The non-selective Rho-kinase inhibitors Y-27632 and Y-33075 decrease contraction but increase migration in murine and human hepatic stellate cells
by: Nadine Bachtler, et al.
Published: (2023-01-01)

The effects of Rho-associated kinase inhibitor Y-27632 on primary human corneal endothelial cells propagated using a dual media approach
by: Peh, Gary S. L., et al.
Published: (2015)

Establishment of an Efficient Primary Culture System for Human Hair Follicle Stem Cells Using the Rho-Associated Protein Kinase Inhibitor Y-27632
by: Lihong Wen, et al.
Published: (2021-03-01)

Influence of Rho Kinase Inhibitor Fasudil on Late Endothelial Progenitor Cells in Peripheral Blood of COPD Patients with Pulmonary Artery Hypertension
by: Pei Liu, et al.
Published: (2014-05-01)

Fasudil improves endothelial dysfunction in rats exposed to chronic intermittent hypoxia through RhoA/ROCK/NFATc3 pathway.
by: Jie-Ru Li, et al.
Published: (2018-01-01)

Fasudil inhibits actin polymerization and collagen synthesis and induces apoptosis in human urethral scar fibroblasts via the Rho/ROCK pathway
by: Li XD, et al.
Published: (2018-09-01)

Effect of Y-27632 on the cultured retinal neurocytes of rats
by: Pei-Li Feng
Published: (2013-02-01)

Inhibition of Rho Kinase by Fasudil Ameliorates Cognition Impairment in APP/PS1 Transgenic Mice via Modulation of Gut Microbiota and Metabolites
by: Yuqing Yan, et al.
Published: (2021-10-01)

Combination of fasudil and celecoxib promotes the recovery of injured spinal cord in rats better than celecoxib or fasudil alone
by: Xiao-lin Hou, et al.
Published: (2015-01-01)

Therapeutic Potency of Induced Pluripotent Stem-Cell-Derived Corneal Endothelial-like Cells for Corneal Endothelial Dysfunction
by: Seongjun So, et al.
Published: (2022-12-01)

Y-27632 Induces Neurite Outgrowth by Activating the NOX1-Mediated AKT and PAK1 Phosphorylation Cascades in PC12 Cells
by: So Yeong Park, et al.
Published: (2020-10-01)

Limitations of Preimplantation Genetic Diagnosis for Mitochondrial DNA Diseases
by: Shoukhrat Mitalipov, et al.
Published: (2014-05-01)

A Rho-associated coiled-coil containing kinases (ROCK) inhibitor, Y-27632, enhances adhesion, viability and differentiation of human term placenta-derived trophoblasts in vitro.
by: Kenichiro Motomura, et al.
Published: (2017-01-01)