Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat ()
As a member of a subclass of immunophilins, it is controversial that FKBP38 acts an upstream regulator of mTOR signaling pathway, which control the process of cell-growth, proliferation and differentiation. In order to explore the relationship between FKBP38 and mTOR in the Cashmere goat (Capra hirc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2012-06-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/25-98.pdf |
| _version_ | 1828769639671267328 |
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| author | X. Zheng X. Y. Hao Y. H. Chen X. Zhang J. F. Yang Z. G. Wang D. J. Liu |
| author_facet | X. Zheng X. Y. Hao Y. H. Chen X. Zhang J. F. Yang Z. G. Wang D. J. Liu |
| author_sort | X. Zheng |
| collection | DOAJ |
| description | As a member of a subclass of immunophilins, it is controversial that FKBP38 acts an upstream regulator of mTOR signaling pathway, which control the process of cell-growth, proliferation and differentiation. In order to explore the relationship between FKBP38 and mTOR in the Cashmere goat (Capra hircus) cells, a full-length cDNA was cloned (GenBank accession number JF714970) and expression pattern was analyzed. The cloned FKBP38 gene is 1,248 bp in length, containing an open reading frame (ORF) from nucleotide 13 to 1,248 which encodes 411 amino acids, and 12 nucleotides in front of the initiation codon. The full cDNA sequence shares 98% identity with cattle, 94% with horse and 90% with human. The putative amino acid sequence shows the higher homology which is 98%, 97% and 94%, correspondingly. The bioinformatics analysis showed that FKBP38 contained a FKBP_C domain, two TPR domains and a TM domain. Psite analysis suggested that the ORF encoding protein contained a leucine-zipper pattern and a Prenyl group binding site (CAAX box). Tissue-specific expression analysis was performed by semi-quantitative RT-PCR and showed that the FKBP38 expression was detected in all the tested tissues and the highest level of mRNA accumulation was detected in testis, suggesting that FKBP38 plays an important role in goat cells. |
| first_indexed | 2024-12-11T13:52:33Z |
| format | Article |
| id | doaj.art-7a268e092b61425f85d67b9ed6b5fcbd |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-11T13:52:33Z |
| publishDate | 2012-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-7a268e092b61425f85d67b9ed6b5fcbd2022-12-22T01:04:13ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172012-06-0125675876310.5713/ajas.2011.1139822741Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat ()X. Zheng0X. Y. Hao1Y. H. Chen2X. Zhang3J. F. Yang4Z. G. Wang5D. J. Liu6 College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China College of Life Science, Inner Mongolia University, The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, ChinaAs a member of a subclass of immunophilins, it is controversial that FKBP38 acts an upstream regulator of mTOR signaling pathway, which control the process of cell-growth, proliferation and differentiation. In order to explore the relationship between FKBP38 and mTOR in the Cashmere goat (Capra hircus) cells, a full-length cDNA was cloned (GenBank accession number JF714970) and expression pattern was analyzed. The cloned FKBP38 gene is 1,248 bp in length, containing an open reading frame (ORF) from nucleotide 13 to 1,248 which encodes 411 amino acids, and 12 nucleotides in front of the initiation codon. The full cDNA sequence shares 98% identity with cattle, 94% with horse and 90% with human. The putative amino acid sequence shows the higher homology which is 98%, 97% and 94%, correspondingly. The bioinformatics analysis showed that FKBP38 contained a FKBP_C domain, two TPR domains and a TM domain. Psite analysis suggested that the ORF encoding protein contained a leucine-zipper pattern and a Prenyl group binding site (CAAX box). Tissue-specific expression analysis was performed by semi-quantitative RT-PCR and showed that the FKBP38 expression was detected in all the tested tissues and the highest level of mRNA accumulation was detected in testis, suggesting that FKBP38 plays an important role in goat cells.http://www.ajas.info/upload/pdf/25-98.pdfCashmere GoatExpression PatternTissue-specificGene |
| spellingShingle | X. Zheng X. Y. Hao Y. H. Chen X. Zhang J. F. Yang Z. G. Wang D. J. Liu Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat () Asian-Australasian Journal of Animal Sciences Cashmere Goat Expression Pattern Tissue-specific Gene |
| title | Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat () |
| title_full | Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat () |
| title_fullStr | Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat () |
| title_full_unstemmed | Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat () |
| title_short | Molecular Characterization and Tissue-specific Expression of a Novel FKBP38 Gene in the Cashmere Goat () |
| title_sort | molecular characterization and tissue specific expression of a novel fkbp38 gene in the cashmere goat |
| topic | Cashmere Goat Expression Pattern Tissue-specific Gene |
| url | http://www.ajas.info/upload/pdf/25-98.pdf |
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