Partial Simultaneous Saccharification and Fermentation at High Solids Loadings of Alkaline-pretreated Miscanthus for Bioethanol Production
In this study, alkaline pretreatment at a bench scale (15-L capacity) was performed to obtain a higher solid residue for the SSF (simultaneous saccharification and fermentation) of Miscanthus sacchariflorus “Goedae-Uksae 1” (GU) under the following conditions: 1 M NaOH concentration, 150 °C, and 60...
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North Carolina State University
2014-09-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6899_Cha_Partial_Simultaneous_Saccharification |
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| author | Young-Lok Cha Gi Hong An Yuri Park Jungwoo Yang Jong-Woong An Youn-Ho Moon Young-Mi Yoon Gyeong-Dan Yu In-Hu Choi |
| author_facet | Young-Lok Cha Gi Hong An Yuri Park Jungwoo Yang Jong-Woong An Youn-Ho Moon Young-Mi Yoon Gyeong-Dan Yu In-Hu Choi |
| author_sort | Young-Lok Cha |
| collection | DOAJ |
| description | In this study, alkaline pretreatment at a bench scale (15-L capacity) was performed to obtain a higher solid residue for the SSF (simultaneous saccharification and fermentation) of Miscanthus sacchariflorus “Goedae-Uksae 1” (GU) under the following conditions: 1 M NaOH concentration, 150 °C, and 60 min residence time. Compositional analysis and scanning electron microscope analysis revealed the pretreatment to be highly effective for achieving delignification and morphological changes. Spiral impellers were used for the rapid liquefaction of pretreated GU into slurry, and no additional nutrients were added to the fermentation mixture to reduce overall process costs. The SSF was subsequently conducted in a laboratory-scale fermenter (5-L capacity) for 108 to 120 h with 12% and 16% glucan containing pretreated GU. Consequently, 62.8 g/L and 81.1 g/L of ethanol were obtained. Based on these data, the theoretical ethanol yields from 1 kg of GU (dry weight base) were estimated at 164.6 to 171.1 g/L. |
| first_indexed | 2024-12-11T21:24:50Z |
| format | Article |
| id | doaj.art-7a293701f4434f03b2f9561a0d98ea6b |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-11T21:24:50Z |
| publishDate | 2014-09-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-7a293701f4434f03b2f9561a0d98ea6b2022-12-22T00:50:21ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-09-01946899691310.15376/biores.9.4.6899-6913Partial Simultaneous Saccharification and Fermentation at High Solids Loadings of Alkaline-pretreated Miscanthus for Bioethanol ProductionYoung-Lok Cha0Gi Hong An1Yuri Park2Jungwoo Yang3Jong-Woong An4Youn-Ho Moon5Young-Mi Yoon6Gyeong-Dan Yu7In-Hu Choi8Bioenergy Crop Research Center National Institute of Crop Science, Rural Development Administration; Korea, Republic OfBioenergy Crop Research Center National Institute of Crop Science, Rural Development Administration; Korea, Republic OfBioenergy Crop Research Center National Institute of Crop Science, Rural Development Administration; Korea, Republic OfSchool of Life Sciences & Biotechnology for BK21 PLUS Department of Biotechnology Food Bioenigeering Lab Korea University; Korea, Republic OfBioenergy Crop Research Center National Institute of Crop Science, Rural Development Administration; Korea, Republic OfBioenergy Crop Research Center National Institute of Crop Science, Rural Development Administration; Korea, Republic OfBioenergy Crop Research Center National Institute of Crop Science, Rural Development Administration; Korea, Republic OfBioenergy Crop Research Center National Institute of Crop Science, Rural Development Administration; Korea, Republic OfBioenergy Crop Research Center National Institute of Crop Science, Rural Development Administration; Korea, Republic OfIn this study, alkaline pretreatment at a bench scale (15-L capacity) was performed to obtain a higher solid residue for the SSF (simultaneous saccharification and fermentation) of Miscanthus sacchariflorus “Goedae-Uksae 1” (GU) under the following conditions: 1 M NaOH concentration, 150 °C, and 60 min residence time. Compositional analysis and scanning electron microscope analysis revealed the pretreatment to be highly effective for achieving delignification and morphological changes. Spiral impellers were used for the rapid liquefaction of pretreated GU into slurry, and no additional nutrients were added to the fermentation mixture to reduce overall process costs. The SSF was subsequently conducted in a laboratory-scale fermenter (5-L capacity) for 108 to 120 h with 12% and 16% glucan containing pretreated GU. Consequently, 62.8 g/L and 81.1 g/L of ethanol were obtained. Based on these data, the theoretical ethanol yields from 1 kg of GU (dry weight base) were estimated at 164.6 to 171.1 g/L.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6899_Cha_Partial_Simultaneous_SaccharificationAlkali pretreatmentBioethanolHigh solids loadingMiscanthusSimultaneous saccharification and fermentation |
| spellingShingle | Young-Lok Cha Gi Hong An Yuri Park Jungwoo Yang Jong-Woong An Youn-Ho Moon Young-Mi Yoon Gyeong-Dan Yu In-Hu Choi Partial Simultaneous Saccharification and Fermentation at High Solids Loadings of Alkaline-pretreated Miscanthus for Bioethanol Production BioResources Alkali pretreatment Bioethanol High solids loading Miscanthus Simultaneous saccharification and fermentation |
| title | Partial Simultaneous Saccharification and Fermentation at High Solids Loadings of Alkaline-pretreated Miscanthus for Bioethanol Production |
| title_full | Partial Simultaneous Saccharification and Fermentation at High Solids Loadings of Alkaline-pretreated Miscanthus for Bioethanol Production |
| title_fullStr | Partial Simultaneous Saccharification and Fermentation at High Solids Loadings of Alkaline-pretreated Miscanthus for Bioethanol Production |
| title_full_unstemmed | Partial Simultaneous Saccharification and Fermentation at High Solids Loadings of Alkaline-pretreated Miscanthus for Bioethanol Production |
| title_short | Partial Simultaneous Saccharification and Fermentation at High Solids Loadings of Alkaline-pretreated Miscanthus for Bioethanol Production |
| title_sort | partial simultaneous saccharification and fermentation at high solids loadings of alkaline pretreated miscanthus for bioethanol production |
| topic | Alkali pretreatment Bioethanol High solids loading Miscanthus Simultaneous saccharification and fermentation |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6899_Cha_Partial_Simultaneous_Saccharification |
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