Canonical and non-canonical roles for ATG8 proteins in autophagy and beyond
During autophagy, the ATG8 family proteins have several well-characterized roles in facilitating early, mid, and late steps of autophagy, including autophagosome expansion, cargo recruitment and autophagosome-lysosome fusion. Their discovery has importantly allowed for precise experimental monitorin...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.1074701/full |
| _version_ | 1811197832082751488 |
|---|---|
| author | Steven Edward Reid Srinivasa Prasad Kolapalli Thorbjørn M. Nielsen Lisa B. Frankel Lisa B. Frankel |
| author_facet | Steven Edward Reid Srinivasa Prasad Kolapalli Thorbjørn M. Nielsen Lisa B. Frankel Lisa B. Frankel |
| author_sort | Steven Edward Reid |
| collection | DOAJ |
| description | During autophagy, the ATG8 family proteins have several well-characterized roles in facilitating early, mid, and late steps of autophagy, including autophagosome expansion, cargo recruitment and autophagosome-lysosome fusion. Their discovery has importantly allowed for precise experimental monitoring of the pathway, bringing about a huge expansion of research in the field over the last decades. In this review, we discuss both canonical and non-canonical roles of the autophagic lipidation machinery, with particular focus on the ATG8 proteins, their post-translational modifications and their increasingly uncovered alternative roles mediated through their anchoring at different membranes. These include endosomes, macropinosomes, phagosomes and the plasma membrane, to which ATG8 proteins can bind through canonical or alternative lipidation. Beyond new ATG8 binding partners and cargo types, we also explore several open questions related to alternative outcomes of autophagic machinery engagement beyond degradation. These include their roles in plasma membrane repair and secretion of selected substrates as well as the physiological implications hereof in health and disease. |
| first_indexed | 2024-04-12T01:21:05Z |
| format | Article |
| id | doaj.art-7a29b0861c384274acff9dc3cfa266a8 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-12T01:21:05Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-7a29b0861c384274acff9dc3cfa266a82022-12-22T03:53:48ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-12-01910.3389/fmolb.2022.10747011074701Canonical and non-canonical roles for ATG8 proteins in autophagy and beyondSteven Edward Reid0Srinivasa Prasad Kolapalli1Thorbjørn M. Nielsen2Lisa B. Frankel3Lisa B. Frankel4Danish Cancer Society Research Center, Copenhagen, DenmarkDanish Cancer Society Research Center, Copenhagen, DenmarkDanish Cancer Society Research Center, Copenhagen, DenmarkDanish Cancer Society Research Center, Copenhagen, DenmarkBiotech Research and Innovation Center, University of Copenhagen, Copenhagen, DenmarkDuring autophagy, the ATG8 family proteins have several well-characterized roles in facilitating early, mid, and late steps of autophagy, including autophagosome expansion, cargo recruitment and autophagosome-lysosome fusion. Their discovery has importantly allowed for precise experimental monitoring of the pathway, bringing about a huge expansion of research in the field over the last decades. In this review, we discuss both canonical and non-canonical roles of the autophagic lipidation machinery, with particular focus on the ATG8 proteins, their post-translational modifications and their increasingly uncovered alternative roles mediated through their anchoring at different membranes. These include endosomes, macropinosomes, phagosomes and the plasma membrane, to which ATG8 proteins can bind through canonical or alternative lipidation. Beyond new ATG8 binding partners and cargo types, we also explore several open questions related to alternative outcomes of autophagic machinery engagement beyond degradation. These include their roles in plasma membrane repair and secretion of selected substrates as well as the physiological implications hereof in health and disease.https://www.frontiersin.org/articles/10.3389/fmolb.2022.1074701/fullautophagylipidationAtg8post-translational modification (PTM)secretory autophagysingle membrane |
| spellingShingle | Steven Edward Reid Srinivasa Prasad Kolapalli Thorbjørn M. Nielsen Lisa B. Frankel Lisa B. Frankel Canonical and non-canonical roles for ATG8 proteins in autophagy and beyond Frontiers in Molecular Biosciences autophagy lipidation Atg8 post-translational modification (PTM) secretory autophagy single membrane |
| title | Canonical and non-canonical roles for ATG8 proteins in autophagy and beyond |
| title_full | Canonical and non-canonical roles for ATG8 proteins in autophagy and beyond |
| title_fullStr | Canonical and non-canonical roles for ATG8 proteins in autophagy and beyond |
| title_full_unstemmed | Canonical and non-canonical roles for ATG8 proteins in autophagy and beyond |
| title_short | Canonical and non-canonical roles for ATG8 proteins in autophagy and beyond |
| title_sort | canonical and non canonical roles for atg8 proteins in autophagy and beyond |
| topic | autophagy lipidation Atg8 post-translational modification (PTM) secretory autophagy single membrane |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.1074701/full |
| work_keys_str_mv | AT stevenedwardreid canonicalandnoncanonicalrolesforatg8proteinsinautophagyandbeyond AT srinivasaprasadkolapalli canonicalandnoncanonicalrolesforatg8proteinsinautophagyandbeyond AT thorbjørnmnielsen canonicalandnoncanonicalrolesforatg8proteinsinautophagyandbeyond AT lisabfrankel canonicalandnoncanonicalrolesforatg8proteinsinautophagyandbeyond AT lisabfrankel canonicalandnoncanonicalrolesforatg8proteinsinautophagyandbeyond |