Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria
Nowadays, it is a great challenge to develop new medicines for treating various infectious diseases. The treatment of these diseases is of utmost interest to further prevent the development of multi-drug resistance in different pathogens. Carbon quantum dots, as a new member of the carbon nanomateri...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/5/919 |
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| author | Zoran M. Marković Aleksandra S. Mišović Danica Z. Zmejkoski Nemanja M. Zdravković Janez Kovač Danica V. Bajuk-Bogdanović Dušan D. Milivojević Marija M. Mojsin Milena J. Stevanović Vladimir B. Pavlović Biljana M. Todorović Marković |
| author_facet | Zoran M. Marković Aleksandra S. Mišović Danica Z. Zmejkoski Nemanja M. Zdravković Janez Kovač Danica V. Bajuk-Bogdanović Dušan D. Milivojević Marija M. Mojsin Milena J. Stevanović Vladimir B. Pavlović Biljana M. Todorović Marković |
| author_sort | Zoran M. Marković |
| collection | DOAJ |
| description | Nowadays, it is a great challenge to develop new medicines for treating various infectious diseases. The treatment of these diseases is of utmost interest to further prevent the development of multi-drug resistance in different pathogens. Carbon quantum dots, as a new member of the carbon nanomaterials family, can potentially be used as a highly promising visible-light-triggered antibacterial agent. In this work, the results of antibacterial and cytotoxic activities of gamma-ray-irradiated carbon quantum dots are presented. Carbon quantum dots (CQDs) were synthesized from citric acid by a pyrolysis procedure and irradiated by gamma rays at different doses (25, 50, 100 and 200 kGy). Structure, chemical composition and optical properties were investigated by atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, UV-Vis spectrometry and photoluminescence. Structural analysis showed that CQDs have a spherical-like shape and dose-dependent average diameters and heights. Antibacterial tests showed that all irradiated dots had antibacterial activity but CQDs irradiated with dose of 100 kGy had antibacterial activity against all seven pathogen-reference bacterial strains. Gamma-ray-modified CQDs did not show any cytotoxicity toward human fetal-originated MRC-5 cells. Moreover, fluorescence microscopy showed excellent cellular uptake of CQDs irradiated with doses of 25 and 200 kGy into MRC-5 cells. |
| first_indexed | 2024-03-11T03:59:39Z |
| format | Article |
| id | doaj.art-7a2a314567dd441985dc977abb60c870 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T03:59:39Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-7a2a314567dd441985dc977abb60c8702023-11-18T00:13:17ZengMDPI AGAntibiotics2079-63822023-05-0112591910.3390/antibiotics12050919Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of BacteriaZoran M. Marković0Aleksandra S. Mišović1Danica Z. Zmejkoski2Nemanja M. Zdravković3Janez Kovač4Danica V. Bajuk-Bogdanović5Dušan D. Milivojević6Marija M. Mojsin7Milena J. Stevanović8Vladimir B. Pavlović9Biljana M. Todorović Marković10Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Mike Alasa 12-14, 11001 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Mike Alasa 12-14, 11001 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Mike Alasa 12-14, 11001 Belgrade, SerbiaScientific Veterinary Institute of Serbia, Janisa Janulisa 14, 11107 Belgrade, SerbiaJozef Stefan Institute, Department of Surface Engineering—F4, Jamova cesta 39, 1000 Ljubljana, SloveniaFaculty of Physical Chemistry, University of Belgrade, Studentski trg 12-16, 11158 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Mike Alasa 12-14, 11001 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, Nemanjina 6, Zemun, 11080 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Mike Alasa 12-14, 11001 Belgrade, SerbiaNowadays, it is a great challenge to develop new medicines for treating various infectious diseases. The treatment of these diseases is of utmost interest to further prevent the development of multi-drug resistance in different pathogens. Carbon quantum dots, as a new member of the carbon nanomaterials family, can potentially be used as a highly promising visible-light-triggered antibacterial agent. In this work, the results of antibacterial and cytotoxic activities of gamma-ray-irradiated carbon quantum dots are presented. Carbon quantum dots (CQDs) were synthesized from citric acid by a pyrolysis procedure and irradiated by gamma rays at different doses (25, 50, 100 and 200 kGy). Structure, chemical composition and optical properties were investigated by atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, UV-Vis spectrometry and photoluminescence. Structural analysis showed that CQDs have a spherical-like shape and dose-dependent average diameters and heights. Antibacterial tests showed that all irradiated dots had antibacterial activity but CQDs irradiated with dose of 100 kGy had antibacterial activity against all seven pathogen-reference bacterial strains. Gamma-ray-modified CQDs did not show any cytotoxicity toward human fetal-originated MRC-5 cells. Moreover, fluorescence microscopy showed excellent cellular uptake of CQDs irradiated with doses of 25 and 200 kGy into MRC-5 cells.https://www.mdpi.com/2079-6382/12/5/919carbon quantum dotsgamma raysantibacterial activitycellular uptake |
| spellingShingle | Zoran M. Marković Aleksandra S. Mišović Danica Z. Zmejkoski Nemanja M. Zdravković Janez Kovač Danica V. Bajuk-Bogdanović Dušan D. Milivojević Marija M. Mojsin Milena J. Stevanović Vladimir B. Pavlović Biljana M. Todorović Marković Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria Antibiotics carbon quantum dots gamma rays antibacterial activity cellular uptake |
| title | Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria |
| title_full | Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria |
| title_fullStr | Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria |
| title_full_unstemmed | Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria |
| title_short | Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria |
| title_sort | employing gamma ray modified carbon quantum dots to combat a wide range of bacteria |
| topic | carbon quantum dots gamma rays antibacterial activity cellular uptake |
| url | https://www.mdpi.com/2079-6382/12/5/919 |
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