A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells
This study investigated the roles of adiponectin in IVF treatment during Phase I (the basal stage before gonadotropin administration), Phase II (approximately 8 days after gonadotropin administration), and Phase III (on the ovum pick-up day), as well as the effects of adiponectin on <i>CYP19A1...
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MDPI AG
2023-04-01
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| Series: | Life |
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| author | Lixian Qin Chantacha Sitticharoon Somsin Petyim Issarawan Keadkraichaiwat Rungnapa Sririwichitchai Pailin Maikaew Malika Churintaraphan |
| author_facet | Lixian Qin Chantacha Sitticharoon Somsin Petyim Issarawan Keadkraichaiwat Rungnapa Sririwichitchai Pailin Maikaew Malika Churintaraphan |
| author_sort | Lixian Qin |
| collection | DOAJ |
| description | This study investigated the roles of adiponectin in IVF treatment during Phase I (the basal stage before gonadotropin administration), Phase II (approximately 8 days after gonadotropin administration), and Phase III (on the ovum pick-up day), as well as the effects of adiponectin on <i>CYP19A1</i> and the <i>FSH receptor</i> (<i>FSHR</i>) mRNA expression in a human granulosa-like tumor cell line (KGN). In human subjects (a longitudinal study, <i>n</i> = 30), blood samples were collected in all phases, while follicular fluid (FF) was only collected in Phase III. The participants were classified into successful and unsuccessful groups based on the determination of fetal heartbeats. KGN cells were treated with adiponectin/FSH/IGF-1 (an experimental study, <i>n</i> = 3). There was no difference in the adiponectin levels between successful and unsuccessful pregnancies in the FF (Phase III) and in serum (all phases), as well as among the three phases in both groups. Serum FSH (Phase I) was positively associated with serum adiponectin in the unsuccessful group, but it had a negative association in the successful group (all phases). Serum adiponectin and serum FSH (Phase I) were positively correlated in the unsuccessful group, whereas they were negatively correlated (all phases) in the successful group. The serum adiponectin levels (Phase III) were significantly higher than in the FF in unsuccessful pregnancies, but there was no difference in successful pregnancies. FF adiponectin concentrations were negatively correlated with serum LH in successful subjects. In KGN cells, adiponectin had no influence on <i>CYP19A1</i> and <i>FSHR</i> mRNA expression. High adiponectin levels in serum compared to FF (Phase III) in unsuccessful subjects might negatively impact IVF treatment. |
| first_indexed | 2024-03-11T04:50:04Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T04:50:04Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-7a2a45b13ff3485a8a1c80af5ec9a1542023-11-17T20:06:36ZengMDPI AGLife2075-17292023-04-0113499410.3390/life13040994A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa CellsLixian Qin0Chantacha Sitticharoon1Somsin Petyim2Issarawan Keadkraichaiwat3Rungnapa Sririwichitchai4Pailin Maikaew5Malika Churintaraphan6Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Siriraj, Bangkoknoi, Bangkok 10700, ThailandDepartment of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Siriraj, Bangkoknoi, Bangkok 10700, ThailandDepartment of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Siriraj, Bangkoknoi, Bangkok 10700, ThailandDepartment of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Siriraj, Bangkoknoi, Bangkok 10700, ThailandDepartment of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Siriraj, Bangkoknoi, Bangkok 10700, ThailandDepartment of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Siriraj, Bangkoknoi, Bangkok 10700, ThailandDepartment of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Rd., Siriraj, Bangkoknoi, Bangkok 10700, ThailandThis study investigated the roles of adiponectin in IVF treatment during Phase I (the basal stage before gonadotropin administration), Phase II (approximately 8 days after gonadotropin administration), and Phase III (on the ovum pick-up day), as well as the effects of adiponectin on <i>CYP19A1</i> and the <i>FSH receptor</i> (<i>FSHR</i>) mRNA expression in a human granulosa-like tumor cell line (KGN). In human subjects (a longitudinal study, <i>n</i> = 30), blood samples were collected in all phases, while follicular fluid (FF) was only collected in Phase III. The participants were classified into successful and unsuccessful groups based on the determination of fetal heartbeats. KGN cells were treated with adiponectin/FSH/IGF-1 (an experimental study, <i>n</i> = 3). There was no difference in the adiponectin levels between successful and unsuccessful pregnancies in the FF (Phase III) and in serum (all phases), as well as among the three phases in both groups. Serum FSH (Phase I) was positively associated with serum adiponectin in the unsuccessful group, but it had a negative association in the successful group (all phases). Serum adiponectin and serum FSH (Phase I) were positively correlated in the unsuccessful group, whereas they were negatively correlated (all phases) in the successful group. The serum adiponectin levels (Phase III) were significantly higher than in the FF in unsuccessful pregnancies, but there was no difference in successful pregnancies. FF adiponectin concentrations were negatively correlated with serum LH in successful subjects. In KGN cells, adiponectin had no influence on <i>CYP19A1</i> and <i>FSHR</i> mRNA expression. High adiponectin levels in serum compared to FF (Phase III) in unsuccessful subjects might negatively impact IVF treatment.https://www.mdpi.com/2075-1729/13/4/994adiponectinIVF/ICSI treatmentgranulosa cellsaromataseFSH |
| spellingShingle | Lixian Qin Chantacha Sitticharoon Somsin Petyim Issarawan Keadkraichaiwat Rungnapa Sririwichitchai Pailin Maikaew Malika Churintaraphan A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells Life adiponectin IVF/ICSI treatment granulosa cells aromatase FSH |
| title | A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells |
| title_full | A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells |
| title_fullStr | A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells |
| title_full_unstemmed | A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells |
| title_short | A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells |
| title_sort | longitudinal study of the relationship of adiponectin with reproduction in infertile women undergoing ivf icsi treatment and an experimental study in human granulosa cells |
| topic | adiponectin IVF/ICSI treatment granulosa cells aromatase FSH |
| url | https://www.mdpi.com/2075-1729/13/4/994 |
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