PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasis
The potential of PLGA-nanoparticles as a carrier of amphotericin B and doxorubicin against visceral leishmaniasis was evaluated by macrophage-mediated drug targeting approach. PLGA-nanoparticles were modified by coating them with macrophage-specific ligand-lectin. Prior to in-vitro studies, characte...
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| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2017-05-01
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| Series: | Pharmaceutical and Biomedical Research |
| Subjects: | |
| Online Access: | http://pbr.mazums.ac.ir/browse.php?a_code=A-10-100-2&slc_lang=en&sid=1 |
| _version_ | 1818497761281048576 |
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| author | Swati Gupta Prachi sharma |
| author_facet | Swati Gupta Prachi sharma |
| author_sort | Swati Gupta |
| collection | DOAJ |
| description | The potential of PLGA-nanoparticles as a carrier of amphotericin B and doxorubicin against visceral leishmaniasis was evaluated by macrophage-mediated drug targeting approach. PLGA-nanoparticles were modified by coating them with macrophage-specific ligand-lectin. Prior to in-vitro studies, characterization studies were carried out systematically include particle size, surface morphology, percent drug entrapment and percent drug release. In vitro studies were carried out in J774.1 in order to evaluate the effective endocytotic uptake of nanoparticles by macrophages. The antileishmanial activity of PLGA-nanoparticles and lectin-PLGA-nanoparticles was tested in-vitro in leishmania donovani infected macrophage-amastigote system (J774A.1 cells), which showed higher efficacy of lectin grafted PLGA-nanoparticles over plain PLGA-nanoparticles. The prepared plain and lectin grafted PLGA-Nanoparticles based systems showed excellent potential for passive and active intra-macrophage targeting, respectively and the approach could be an effective alternative to the currently available drug regimens against VL. |
| first_indexed | 2024-12-10T18:49:22Z |
| format | Article |
| id | doaj.art-7a2a816e1ed841a2bfe0757c94d5d8cc |
| institution | Directory Open Access Journal |
| issn | 2423-4486 2423-4494 |
| language | English |
| last_indexed | 2024-12-10T18:49:22Z |
| publishDate | 2017-05-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical and Biomedical Research |
| spelling | doaj.art-7a2a816e1ed841a2bfe0757c94d5d8cc2022-12-22T01:37:23ZengMazandaran University of Medical SciencesPharmaceutical and Biomedical Research2423-44862423-44942017-05-01314147PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasisSwati Gupta0Prachi sharma1 Department of Pharmaceutics, B. S. Anangpuria Institute of Pharmacy Apeejay Stya University, Gurgaon, India The potential of PLGA-nanoparticles as a carrier of amphotericin B and doxorubicin against visceral leishmaniasis was evaluated by macrophage-mediated drug targeting approach. PLGA-nanoparticles were modified by coating them with macrophage-specific ligand-lectin. Prior to in-vitro studies, characterization studies were carried out systematically include particle size, surface morphology, percent drug entrapment and percent drug release. In vitro studies were carried out in J774.1 in order to evaluate the effective endocytotic uptake of nanoparticles by macrophages. The antileishmanial activity of PLGA-nanoparticles and lectin-PLGA-nanoparticles was tested in-vitro in leishmania donovani infected macrophage-amastigote system (J774A.1 cells), which showed higher efficacy of lectin grafted PLGA-nanoparticles over plain PLGA-nanoparticles. The prepared plain and lectin grafted PLGA-Nanoparticles based systems showed excellent potential for passive and active intra-macrophage targeting, respectively and the approach could be an effective alternative to the currently available drug regimens against VL.http://pbr.mazums.ac.ir/browse.php?a_code=A-10-100-2&slc_lang=en&sid=1Visceral leishmaniasis macrophage targeting Nanoparticles Amphotericin B Doxorubicin Lectin |
| spellingShingle | Swati Gupta Prachi sharma PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasis Pharmaceutical and Biomedical Research Visceral leishmaniasis macrophage targeting Nanoparticles Amphotericin B Doxorubicin Lectin |
| title | PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasis |
| title_full | PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasis |
| title_fullStr | PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasis |
| title_full_unstemmed | PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasis |
| title_short | PLGA-based macrophage-mediated drug targeting for the treatment of visceral leishmaniasis |
| title_sort | plga based macrophage mediated drug targeting for the treatment of visceral leishmaniasis |
| topic | Visceral leishmaniasis macrophage targeting Nanoparticles Amphotericin B Doxorubicin Lectin |
| url | http://pbr.mazums.ac.ir/browse.php?a_code=A-10-100-2&slc_lang=en&sid=1 |
| work_keys_str_mv | AT swatigupta plgabasedmacrophagemediateddrugtargetingforthetreatmentofvisceralleishmaniasis AT prachisharma plgabasedmacrophagemediateddrugtargetingforthetreatmentofvisceralleishmaniasis |