Histological study on the protective effect of Moringa oleifera leaf extract on indomethacin-induced degenerated testes and uterus in albino rat
Background: Indomethacin has analgesic and antipyretic properties. It is a common treatment for pain and rheumatoid diseases, but it has many side effects. We speculate that the natural antioxidants of Moringa oleifera could be a remedy for the long list of side effects. This study aimed to determin...
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| Format: | Article |
| Language: | English |
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Prathima Institute of Medical Sciences
2023-12-01
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| Series: | Perspectives In Medical Research |
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| Online Access: | https://www.pimr.org.in/2023-vol11-issue-3/originalarticle6_v3.pdf |
| _version_ | 1827386731774607360 |
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| author | Ephron E Yerembede James O. Adisa Solomon M. Gamde David O. Ishola Gloria H. Dachollom Khadija M. Mohammed |
| author_facet | Ephron E Yerembede James O. Adisa Solomon M. Gamde David O. Ishola Gloria H. Dachollom Khadija M. Mohammed |
| author_sort | Ephron E Yerembede |
| collection | DOAJ |
| description | Background: Indomethacin has analgesic and antipyretic properties. It is a common treatment for pain and rheumatoid diseases, but it has many side effects. We speculate that the natural antioxidants of Moringa oleifera could be a remedy for the long list of side effects. This study aimed to determine the possible protective effect of Moringa oleifera leaf extract on the indomethacin-induced degenerated testes and uterus in albino rat.
Methods: Forty-eight white albino rats were assigned randomly into six groups consisting of eight rats. Group, I was the Normal control. Group II was animals induced with indomethacin but not treated. Group III was dosed with 500 mg/kg Moringa oleifera leaf extract while Groups IV-VI were induced with indomethacin and treated with 100, 250, and 500 mg/kg Moringa oleifera leaf extract respectively for 28 days. Animals were euthanized after the treatment period. The uterus and testes were excised and processed using the paraffin wax method and stained with haematoxylin and eosin.
Results: Animals exposed to indomethacin became drowsy and lethargic with deep red teary eyes. The first mortality was recorded among animals administered with indomethacin without treatment. Histopathology of the testes showed that indomethacin caused degeneration of the seminiferous tubules and diminished the spermatozoa. The histology of the uterus was distorted, the endometrium and myometrium layers of the uterus were disintegrated. However, animals treated with Moringa oleifera showed dose-related improvements in the histology of the testes and uterus compared to the control group.
Conclusion: Moringa oleifera leaf extract improved the histology of indomethacin-induced toxicity of the testes and uterine in white albino rats. |
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| issn | 2348-1447 2348-229X |
| language | English |
| last_indexed | 2024-03-08T15:50:49Z |
| publishDate | 2023-12-01 |
| publisher | Prathima Institute of Medical Sciences |
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| series | Perspectives In Medical Research |
| spelling | doaj.art-7a2ab5d48bc84b0387cdd26f8e4b2aa52024-01-09T06:27:00ZengPrathima Institute of Medical SciencesPerspectives In Medical Research2348-14472348-229X2023-12-01113293410.47799/pimr.1103.0606Histological study on the protective effect of Moringa oleifera leaf extract on indomethacin-induced degenerated testes and uterus in albino ratEphron E Yerembede0James O. Adisa1Solomon M. Gamde2David O. Ishola3Gloria H. Dachollom4Khadija M. Mohammed5Department of Medical Laboratory Science, University of Jos, Plateau State, NigeriaDepartment of Medical Laboratory Science, University of Jos, Plateau State, NigeriaDepartment of Medical Laboratory Science, University of Jos, Plateau State, NigeriaDepartment of Vetnary Medicine, Usman Danfodiyo University Sokoto, NigeriaDepartment of Medical Laboratory Science, University of Jos, Plateau State, NigeriaDepartment of Medical Laboratory Science, University of Jos, Plateau State, NigeriaBackground: Indomethacin has analgesic and antipyretic properties. It is a common treatment for pain and rheumatoid diseases, but it has many side effects. We speculate that the natural antioxidants of Moringa oleifera could be a remedy for the long list of side effects. This study aimed to determine the possible protective effect of Moringa oleifera leaf extract on the indomethacin-induced degenerated testes and uterus in albino rat. Methods: Forty-eight white albino rats were assigned randomly into six groups consisting of eight rats. Group, I was the Normal control. Group II was animals induced with indomethacin but not treated. Group III was dosed with 500 mg/kg Moringa oleifera leaf extract while Groups IV-VI were induced with indomethacin and treated with 100, 250, and 500 mg/kg Moringa oleifera leaf extract respectively for 28 days. Animals were euthanized after the treatment period. The uterus and testes were excised and processed using the paraffin wax method and stained with haematoxylin and eosin. Results: Animals exposed to indomethacin became drowsy and lethargic with deep red teary eyes. The first mortality was recorded among animals administered with indomethacin without treatment. Histopathology of the testes showed that indomethacin caused degeneration of the seminiferous tubules and diminished the spermatozoa. The histology of the uterus was distorted, the endometrium and myometrium layers of the uterus were disintegrated. However, animals treated with Moringa oleifera showed dose-related improvements in the histology of the testes and uterus compared to the control group. Conclusion: Moringa oleifera leaf extract improved the histology of indomethacin-induced toxicity of the testes and uterine in white albino rats.https://www.pimr.org.in/2023-vol11-issue-3/originalarticle6_v3.pdfmoringa oleiferaindomethacinhistopathologynsaid |
| spellingShingle | Ephron E Yerembede James O. Adisa Solomon M. Gamde David O. Ishola Gloria H. Dachollom Khadija M. Mohammed Histological study on the protective effect of Moringa oleifera leaf extract on indomethacin-induced degenerated testes and uterus in albino rat Perspectives In Medical Research moringa oleifera indomethacin histopathology nsaid |
| title | Histological study on the protective effect of
Moringa oleifera
leaf extract on indomethacin-induced degenerated testes and uterus in albino rat |
| title_full | Histological study on the protective effect of
Moringa oleifera
leaf extract on indomethacin-induced degenerated testes and uterus in albino rat |
| title_fullStr | Histological study on the protective effect of
Moringa oleifera
leaf extract on indomethacin-induced degenerated testes and uterus in albino rat |
| title_full_unstemmed | Histological study on the protective effect of
Moringa oleifera
leaf extract on indomethacin-induced degenerated testes and uterus in albino rat |
| title_short | Histological study on the protective effect of
Moringa oleifera
leaf extract on indomethacin-induced degenerated testes and uterus in albino rat |
| title_sort | histological study on the protective effect of moringa oleifera leaf extract on indomethacin induced degenerated testes and uterus in albino rat |
| topic | moringa oleifera indomethacin histopathology nsaid |
| url | https://www.pimr.org.in/2023-vol11-issue-3/originalarticle6_v3.pdf |
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