Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and Apoptosis
Background/Aims: Renalase, a novel flavoprotein expressed in the kidney and heart, reduces renal tubular necrosis and apoptosis, which suggests that it might protect against necrosis and/or apoptosis in myocardial ischemia reperfusion injury (MIRI). The present study thus explored the effects of ren...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Karger Publishers
2015-05-01
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| Series: | Kidney & Blood Pressure Research |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/368497 |
| _version_ | 1818912045411598336 |
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| author | Xiaogang Li Zijian Xie Minyuan Lin Ruixia Huang Zhongshu Liang Wei Huang Weihong Jiang |
| author_facet | Xiaogang Li Zijian Xie Minyuan Lin Ruixia Huang Zhongshu Liang Wei Huang Weihong Jiang |
| author_sort | Xiaogang Li |
| collection | DOAJ |
| description | Background/Aims: Renalase, a novel flavoprotein expressed in the kidney and heart, reduces renal tubular necrosis and apoptosis, which suggests that it might protect against necrosis and/or apoptosis in myocardial ischemia reperfusion injury (MIRI). The present study thus explored the effects of renalase on Sprague-Dawley (SD) rats subjected to MIRI. Methods: We used Lentivirus-mediated RNA interference (RNAi) to inhibit the renalase gene expression in the heart tissue via pericardial cavity injection. The MIRI animal modal was established by blocking the left anterior descending artery for 45mins followed by 4h of reperfusion. Real-time PCR and western blotting were used to detect renalase expression in the heart tissue. Double staining and TUNEL were used to detect the necrosis and apoptosis in the myocardial cells, respectively. Results: All rats subjected to MIRI exhibited lower levels of renalase in the heart tissue than did the sham-operated group (PConclusions: Exogenous recombinant renalase protein reduced myocardial cell necrosis and apoptosis. Recombinant renalase protein might be a new cardiovascular drug for ischemia/IR injury. |
| first_indexed | 2024-12-19T23:08:21Z |
| format | Article |
| id | doaj.art-7a2d556084d94e8899fbaf94fa5ad6c4 |
| institution | Directory Open Access Journal |
| issn | 1420-4096 1423-0143 |
| language | English |
| last_indexed | 2024-12-19T23:08:21Z |
| publishDate | 2015-05-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Kidney & Blood Pressure Research |
| spelling | doaj.art-7a2d556084d94e8899fbaf94fa5ad6c42022-12-21T20:02:19ZengKarger PublishersKidney & Blood Pressure Research1420-40961423-01432015-05-0140321522210.1159/000368497368497Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and ApoptosisXiaogang LiZijian XieMinyuan LinRuixia HuangZhongshu LiangWei HuangWeihong JiangBackground/Aims: Renalase, a novel flavoprotein expressed in the kidney and heart, reduces renal tubular necrosis and apoptosis, which suggests that it might protect against necrosis and/or apoptosis in myocardial ischemia reperfusion injury (MIRI). The present study thus explored the effects of renalase on Sprague-Dawley (SD) rats subjected to MIRI. Methods: We used Lentivirus-mediated RNA interference (RNAi) to inhibit the renalase gene expression in the heart tissue via pericardial cavity injection. The MIRI animal modal was established by blocking the left anterior descending artery for 45mins followed by 4h of reperfusion. Real-time PCR and western blotting were used to detect renalase expression in the heart tissue. Double staining and TUNEL were used to detect the necrosis and apoptosis in the myocardial cells, respectively. Results: All rats subjected to MIRI exhibited lower levels of renalase in the heart tissue than did the sham-operated group (PConclusions: Exogenous recombinant renalase protein reduced myocardial cell necrosis and apoptosis. Recombinant renalase protein might be a new cardiovascular drug for ischemia/IR injury.http://www.karger.com/Article/FullText/368497Ischemia and reperfusion injuryLentivirusRNA interferenceRecombinant renalase proteinRenalase |
| spellingShingle | Xiaogang Li Zijian Xie Minyuan Lin Ruixia Huang Zhongshu Liang Wei Huang Weihong Jiang Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and Apoptosis Kidney & Blood Pressure Research Ischemia and reperfusion injury Lentivirus RNA interference Recombinant renalase protein Renalase |
| title | Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and Apoptosis |
| title_full | Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and Apoptosis |
| title_fullStr | Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and Apoptosis |
| title_full_unstemmed | Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and Apoptosis |
| title_short | Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and Apoptosis |
| title_sort | renalase protects the cardiomyocytes of sprague dawley rats against ischemia and reperfusion injury by reducing myocardial cell necrosis and apoptosis |
| topic | Ischemia and reperfusion injury Lentivirus RNA interference Recombinant renalase protein Renalase |
| url | http://www.karger.com/Article/FullText/368497 |
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