Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extracts
Abstract Background Matrix metalloproteinases (MMPs) are linked with several complications such as metastasis of cancer progression, oxidative stress, and hepatic fibrosis. Brown seaweeds are being extensively studied for their bioactive molecule content against cancer progression. In this context,...
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| Format: | Article |
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The Korean Society of Fisheries and Aquatic Science
2018-06-01
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| Series: | Fisheries and Aquatic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s41240-018-0093-0 |
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| author | Fatih Karadeniz Seul-Gi Lee Jung Hwan Oh Jung-Ae Kim Chang-Suk Kong |
| author_facet | Fatih Karadeniz Seul-Gi Lee Jung Hwan Oh Jung-Ae Kim Chang-Suk Kong |
| author_sort | Fatih Karadeniz |
| collection | DOAJ |
| description | Abstract Background Matrix metalloproteinases (MMPs) are linked with several complications such as metastasis of cancer progression, oxidative stress, and hepatic fibrosis. Brown seaweeds are being extensively studied for their bioactive molecule content against cancer progression. In this context, Sargassum horneri was reported to possess various bioactivities including antiviral, antimicrobial, and anti-inflammatory partly due to its phenolic compound content. Methods In this study, potential of S. horneri was evaluated through anti-MMP effect in HT1080 fibrosarcoma cells. S. horneri crude extract was fractionated with organic solvents, namely, water (H2O), n-buthanol (n-BuOH), 85% aqueous methanol (85% aq. MeOH), and n-hexane. The non-toxicity of fraction samples (Sargassum horneri solvent-partitioned extracts (SHEs)) was confirmed by cell-viability assay. SHEs were tested for their ability to inhibit MMP enzymatic activity through gelatin digestion evaluation and cell migration assay. Expressions of MMP-2 and MMP-9 and tissue inhibitors of MMP (TIMPs) were evaluated by reverse transcription and Western blotting. Results All fractions inhibited the enzymatic activities of MMP-2 and MMP-9 according to gelatin zymography. Except H2O fraction, fractions hindered the cell migration significantly. All tested fractions suppressed both mRNA and protein levels of MMP-2, MMP-9, TIMP-1, and TIMP-2. Conclusion Overall, current results suggested that S. horneri has potential to be a good source for anti-MMP agents, and further investigations are underway for better understanding of the action mechanism and isolation and elucidation of the bioactive molecules. |
| first_indexed | 2024-12-17T20:47:36Z |
| format | Article |
| id | doaj.art-7a2da0df61154619bfabc2e35a7ff14d |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-12-17T20:47:36Z |
| publishDate | 2018-06-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-7a2da0df61154619bfabc2e35a7ff14d2022-12-21T21:33:08ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572018-06-012111710.1186/s41240-018-0093-0Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extractsFatih Karadeniz0Seul-Gi Lee1Jung Hwan Oh2Jung-Ae Kim3Chang-Suk Kong4Department of Organic Material Science and Engineering, Pusan National UniversityDepartment of Food and Nutrition, College of Medical and Life Sciences, Silla UniversityDepartment of Food and Nutrition, College of Medical and Life Sciences, Silla UniversityDepartment of Food and Nutrition, College of Medical and Life Sciences, Silla UniversityDepartment of Food and Nutrition, College of Medical and Life Sciences, Silla UniversityAbstract Background Matrix metalloproteinases (MMPs) are linked with several complications such as metastasis of cancer progression, oxidative stress, and hepatic fibrosis. Brown seaweeds are being extensively studied for their bioactive molecule content against cancer progression. In this context, Sargassum horneri was reported to possess various bioactivities including antiviral, antimicrobial, and anti-inflammatory partly due to its phenolic compound content. Methods In this study, potential of S. horneri was evaluated through anti-MMP effect in HT1080 fibrosarcoma cells. S. horneri crude extract was fractionated with organic solvents, namely, water (H2O), n-buthanol (n-BuOH), 85% aqueous methanol (85% aq. MeOH), and n-hexane. The non-toxicity of fraction samples (Sargassum horneri solvent-partitioned extracts (SHEs)) was confirmed by cell-viability assay. SHEs were tested for their ability to inhibit MMP enzymatic activity through gelatin digestion evaluation and cell migration assay. Expressions of MMP-2 and MMP-9 and tissue inhibitors of MMP (TIMPs) were evaluated by reverse transcription and Western blotting. Results All fractions inhibited the enzymatic activities of MMP-2 and MMP-9 according to gelatin zymography. Except H2O fraction, fractions hindered the cell migration significantly. All tested fractions suppressed both mRNA and protein levels of MMP-2, MMP-9, TIMP-1, and TIMP-2. Conclusion Overall, current results suggested that S. horneri has potential to be a good source for anti-MMP agents, and further investigations are underway for better understanding of the action mechanism and isolation and elucidation of the bioactive molecules.http://link.springer.com/article/10.1186/s41240-018-0093-0Brown algaeMMPSargassum horneriTIMP |
| spellingShingle | Fatih Karadeniz Seul-Gi Lee Jung Hwan Oh Jung-Ae Kim Chang-Suk Kong Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extracts Fisheries and Aquatic Sciences Brown algae MMP Sargassum horneri TIMP |
| title | Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extracts |
| title_full | Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extracts |
| title_fullStr | Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extracts |
| title_full_unstemmed | Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extracts |
| title_short | Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extracts |
| title_sort | inhibition of mmp 2 and mmp 9 activities by solvent partitioned sargassum horneri extracts |
| topic | Brown algae MMP Sargassum horneri TIMP |
| url | http://link.springer.com/article/10.1186/s41240-018-0093-0 |
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