Temporary Cultivation and Live Transport with Water Based on Gradient Cooling of Grass Carp (Ctenopharyngodon idellas)
The study investigated the semi-hibernation and critical temperature of grass carp based on gradient cooling, and explore the effects of temperature (12–24 ℃) on the survival of grass carp and the concentration of ammonia nitrogen in the water. The temperature was reduced from room temperature (23–2...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2024-02-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-031.pdf |
| _version_ | 1797256642982576128 |
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| author | HE Jingyi, WEI Ya, CEN Jianwei, HAO Shuxian, CHEN Shengjun, HUANG Hui,ZHAO Yongqiang, WANG Yueqi, YANG Shaoling, LIN Zhi |
| author_facet | HE Jingyi, WEI Ya, CEN Jianwei, HAO Shuxian, CHEN Shengjun, HUANG Hui,ZHAO Yongqiang, WANG Yueqi, YANG Shaoling, LIN Zhi |
| author_sort | HE Jingyi, WEI Ya, CEN Jianwei, HAO Shuxian, CHEN Shengjun, HUANG Hui,ZHAO Yongqiang, WANG Yueqi, YANG Shaoling, LIN Zhi |
| collection | DOAJ |
| description | The study investigated the semi-hibernation and critical temperature of grass carp based on gradient cooling, and explore the effects of temperature (12–24 ℃) on the survival of grass carp and the concentration of ammonia nitrogen in the water. The temperature was reduced from room temperature (23–25 ℃) to the semi-dormant temperature (16 ℃) at cooling rate of 1, 3, or 5 ℃/h. The control group was reared at room temperature, and sampling was conducted after 0, 12, 24, 36, and 48 h. Serum biochemical and antioxidant parameters were measured to obtain the maximum cooling rate for grass crap. The results showed that the semi-dormant temperature of grass carp was 16 ℃, the critical temperature was 6–8 ℃, and the optimal cooling rate was 3 ℃/h. At 16 ℃, grass carp exhibited delayed stress response and decreased metabolism, and lower concentrations of ammonia nitrogen in the water. Except for aspartate aminotransferase level, the maximum values of other serum biochemical indexes at the cooling rate of 3 ℃/h, were significantly lower than those at 1 and 5 ℃/h (P < 0.05). The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) exhibited a trend of initially increasing and then decreasing with survival time, whereas the content of malondialdehyde (MDA) showed an opposite trend, reaching a minimum value of 5.27 mmol/mg after 36 h. Compared to the control group, the contents of all biochemical and antioxidant indexes in the three cooling groups showed significant changes with survival time. Therefore, cooling grass carp at a rate of 3 ℃/h could reduce the stress response and allowed it to enter a semi-hibernation state at a more suitable time. The results from this study provide key parameters for the temporary cultivation and transport of grass carp, as well as useful guidelines for reducing the transport mortality. |
| first_indexed | 2024-04-24T22:25:00Z |
| format | Article |
| id | doaj.art-7a2e390ad7ce48349413c3801c4814bb |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-04-24T22:25:00Z |
| publishDate | 2024-02-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-7a2e390ad7ce48349413c3801c4814bb2024-03-20T02:27:16ZengChina Food Publishing CompanyShipin Kexue1002-66302024-02-0145427127810.7506/spkx1002-6630-20230411-098Temporary Cultivation and Live Transport with Water Based on Gradient Cooling of Grass Carp (Ctenopharyngodon idellas)HE Jingyi, WEI Ya, CEN Jianwei, HAO Shuxian, CHEN Shengjun, HUANG Hui,ZHAO Yongqiang, WANG Yueqi, YANG Shaoling, LIN Zhi0(1. College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China; 2. Key Lab of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 3. Tropical Fisheries Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Science, Sanya 572019, China; 4. Guangdong Shunxin Sea Fishery Group Co. Ltd., Yangjiang 529800, China)The study investigated the semi-hibernation and critical temperature of grass carp based on gradient cooling, and explore the effects of temperature (12–24 ℃) on the survival of grass carp and the concentration of ammonia nitrogen in the water. The temperature was reduced from room temperature (23–25 ℃) to the semi-dormant temperature (16 ℃) at cooling rate of 1, 3, or 5 ℃/h. The control group was reared at room temperature, and sampling was conducted after 0, 12, 24, 36, and 48 h. Serum biochemical and antioxidant parameters were measured to obtain the maximum cooling rate for grass crap. The results showed that the semi-dormant temperature of grass carp was 16 ℃, the critical temperature was 6–8 ℃, and the optimal cooling rate was 3 ℃/h. At 16 ℃, grass carp exhibited delayed stress response and decreased metabolism, and lower concentrations of ammonia nitrogen in the water. Except for aspartate aminotransferase level, the maximum values of other serum biochemical indexes at the cooling rate of 3 ℃/h, were significantly lower than those at 1 and 5 ℃/h (P < 0.05). The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) exhibited a trend of initially increasing and then decreasing with survival time, whereas the content of malondialdehyde (MDA) showed an opposite trend, reaching a minimum value of 5.27 mmol/mg after 36 h. Compared to the control group, the contents of all biochemical and antioxidant indexes in the three cooling groups showed significant changes with survival time. Therefore, cooling grass carp at a rate of 3 ℃/h could reduce the stress response and allowed it to enter a semi-hibernation state at a more suitable time. The results from this study provide key parameters for the temporary cultivation and transport of grass carp, as well as useful guidelines for reducing the transport mortality.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-031.pdflow-temperature hibernation; cooling rate; stress; biochemical indicators; antioxidant indicators |
| spellingShingle | HE Jingyi, WEI Ya, CEN Jianwei, HAO Shuxian, CHEN Shengjun, HUANG Hui,ZHAO Yongqiang, WANG Yueqi, YANG Shaoling, LIN Zhi Temporary Cultivation and Live Transport with Water Based on Gradient Cooling of Grass Carp (Ctenopharyngodon idellas) Shipin Kexue low-temperature hibernation; cooling rate; stress; biochemical indicators; antioxidant indicators |
| title | Temporary Cultivation and Live Transport with Water Based on Gradient Cooling of Grass Carp (Ctenopharyngodon idellas) |
| title_full | Temporary Cultivation and Live Transport with Water Based on Gradient Cooling of Grass Carp (Ctenopharyngodon idellas) |
| title_fullStr | Temporary Cultivation and Live Transport with Water Based on Gradient Cooling of Grass Carp (Ctenopharyngodon idellas) |
| title_full_unstemmed | Temporary Cultivation and Live Transport with Water Based on Gradient Cooling of Grass Carp (Ctenopharyngodon idellas) |
| title_short | Temporary Cultivation and Live Transport with Water Based on Gradient Cooling of Grass Carp (Ctenopharyngodon idellas) |
| title_sort | temporary cultivation and live transport with water based on gradient cooling of grass carp ctenopharyngodon idellas |
| topic | low-temperature hibernation; cooling rate; stress; biochemical indicators; antioxidant indicators |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-031.pdf |
| work_keys_str_mv | AT hejingyiweiyacenjianweihaoshuxianchenshengjunhuanghuizhaoyongqiangwangyueqiyangshaolinglinzhi temporarycultivationandlivetransportwithwaterbasedongradientcoolingofgrasscarpctenopharyngodonidellas |