Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from <i>Secale cereale</i> L. Qinling with Resistance to Stripe Rust and Powdery Mildew
In this study, a novel T6RS.6AL translocation line, 117-6, was selected from a cross between common Chuannong25 (CN25) wheat and Qinling rye. The results of nondenaturing fluorescence in situ hybridization (ND-FISH) and PCR showed that 117-6 contained two T6RS.6AL translocation chromosomes. The dist...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10495 |
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| author | Tianheng Ren Zixin Sun Zhenglong Ren Feiquan Tan Peigao Luo Zhi Li |
| author_facet | Tianheng Ren Zixin Sun Zhenglong Ren Feiquan Tan Peigao Luo Zhi Li |
| author_sort | Tianheng Ren |
| collection | DOAJ |
| description | In this study, a novel T6RS.6AL translocation line, 117-6, was selected from a cross between common Chuannong25 (CN25) wheat and Qinling rye. The results of nondenaturing fluorescence in situ hybridization (ND-FISH) and PCR showed that 117-6 contained two T6RS.6AL translocation chromosomes. The distal region of the 6RS chromosome in 117-6 was mutant and showed different FISH signal patterns. When inoculated with different stripe rust races and powdery mildew races in seedlings, 117-6 expressed high resistance to them. The 117-6 line also exhibited high resistance to stripe rust and powdery mildew in the field under natural <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>) and <i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>) infection. The cytogenetic analysis indicated that the introduction of 6RS conferred resistance ability. Compared with wheat parent CN25, 117-6 exhibited excellent agronomic traits in the field. The present study indicated that Qinling rye may carry favorite genes as a potential source for wheat genetic improvement, and 117-6 could be a useful germplasm for wheat breeding programs in the future. |
| first_indexed | 2024-03-09T23:46:48Z |
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| id | doaj.art-7a309f75eea84e5b9dc5ef2f775d36b7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:46:48Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7a309f75eea84e5b9dc5ef2f775d36b72023-11-23T16:42:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181049510.3390/ijms231810495Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from <i>Secale cereale</i> L. Qinling with Resistance to Stripe Rust and Powdery MildewTianheng Ren0Zixin Sun1Zhenglong Ren2Feiquan Tan3Peigao Luo4Zhi Li5State key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaState key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaIn this study, a novel T6RS.6AL translocation line, 117-6, was selected from a cross between common Chuannong25 (CN25) wheat and Qinling rye. The results of nondenaturing fluorescence in situ hybridization (ND-FISH) and PCR showed that 117-6 contained two T6RS.6AL translocation chromosomes. The distal region of the 6RS chromosome in 117-6 was mutant and showed different FISH signal patterns. When inoculated with different stripe rust races and powdery mildew races in seedlings, 117-6 expressed high resistance to them. The 117-6 line also exhibited high resistance to stripe rust and powdery mildew in the field under natural <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>) and <i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>) infection. The cytogenetic analysis indicated that the introduction of 6RS conferred resistance ability. Compared with wheat parent CN25, 117-6 exhibited excellent agronomic traits in the field. The present study indicated that Qinling rye may carry favorite genes as a potential source for wheat genetic improvement, and 117-6 could be a useful germplasm for wheat breeding programs in the future.https://www.mdpi.com/1422-0067/23/18/10495wheatchromosome translocation6RS.6ALQinling ryeND-FISHdisease resistance |
| spellingShingle | Tianheng Ren Zixin Sun Zhenglong Ren Feiquan Tan Peigao Luo Zhi Li Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from <i>Secale cereale</i> L. Qinling with Resistance to Stripe Rust and Powdery Mildew International Journal of Molecular Sciences wheat chromosome translocation 6RS.6AL Qinling rye ND-FISH disease resistance |
| title | Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from <i>Secale cereale</i> L. Qinling with Resistance to Stripe Rust and Powdery Mildew |
| title_full | Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from <i>Secale cereale</i> L. Qinling with Resistance to Stripe Rust and Powdery Mildew |
| title_fullStr | Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from <i>Secale cereale</i> L. Qinling with Resistance to Stripe Rust and Powdery Mildew |
| title_full_unstemmed | Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from <i>Secale cereale</i> L. Qinling with Resistance to Stripe Rust and Powdery Mildew |
| title_short | Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line from <i>Secale cereale</i> L. Qinling with Resistance to Stripe Rust and Powdery Mildew |
| title_sort | development and molecular cytogenetic characterization of a novel wheat rye t6rs 6al translocation line from i secale cereale i l qinling with resistance to stripe rust and powdery mildew |
| topic | wheat chromosome translocation 6RS.6AL Qinling rye ND-FISH disease resistance |
| url | https://www.mdpi.com/1422-0067/23/18/10495 |
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