Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China
The continuous expansion of freshwater fish culture is confronted with environmental problems owing to their high antibiotic inputs and antibiotic-resistant bacteria. This study explored the antibiotic resistance gene (ARG) profiles of water and fish gut contents from three fishponds in Tianjin usin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Aquaculture Journal |
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| Online Access: | https://www.mdpi.com/2673-9496/2/4/16 |
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| author | Qiushui Wang Xin Zhou Yue Liu Qi Ding Zan Wu Jie Deng Jia Zuo Liyan Yuan Peng Shao Bo Cheng Lijuan Gao |
| author_facet | Qiushui Wang Xin Zhou Yue Liu Qi Ding Zan Wu Jie Deng Jia Zuo Liyan Yuan Peng Shao Bo Cheng Lijuan Gao |
| author_sort | Qiushui Wang |
| collection | DOAJ |
| description | The continuous expansion of freshwater fish culture is confronted with environmental problems owing to their high antibiotic inputs and antibiotic-resistant bacteria. This study explored the antibiotic resistance gene (ARG) profiles of water and fish gut contents from three fishponds in Tianjin using a metagenomics approach. Proteobacteria, Bacteroidetes, and Actinobacteria were the most abundant phyla in all water samples. However, the microbial composition of the fish guts was distinctly different among the three aquaculture farms. <i>Cetobacterium</i>, <i>Bacillus</i>, <i>Weissella</i>, and <i>Fusobacterium</i> were the dominant genera in the gut contents of all fish. More than 20 unique ARGs with relatively high abundances were detected in both water and fish gut content samples. The dominant genes and pathways of antibiotic resistance mechanisms detected in all samples were antibiotic efflux, antibiotic inactivation, antibiotic target alteration, antibiotic target protection, antibiotic target replacement, and reduced permeability to antibiotics. In addition, our results indicate that antibiotics, such as florfenicol, and heavy metals, such as Zn and Cu, could have a significant correlation with some common ARGs, indicating that antibiotic-resistant bacteria could co-occur with heavy metals. Our study provides a research basis for the development of a strategy for practical antibiotic application and heavy metal monitoring in aquaculture. |
| first_indexed | 2024-03-11T07:29:49Z |
| format | Article |
| id | doaj.art-7a3326c56e66498e83aff983c3f1c7a4 |
| institution | Directory Open Access Journal |
| issn | 2673-9496 |
| language | English |
| last_indexed | 2024-03-11T07:29:49Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Aquaculture Journal |
| spelling | doaj.art-7a3326c56e66498e83aff983c3f1c7a42023-11-17T07:22:59ZengMDPI AGAquaculture Journal2673-94962022-11-012426928410.3390/aquacj2040016Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, ChinaQiushui Wang0Xin Zhou1Yue Liu2Qi Ding3Zan Wu4Jie Deng5Jia Zuo6Liyan Yuan7Peng Shao8Bo Cheng9Lijuan Gao10Institute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaAquatic Products Quality and Standards Research Center, Chinese Academy of Fishery Sciences, Beijing 100141, ChinaInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center for Physical and Chemical Analysis, Beijing 100089, ChinaThe continuous expansion of freshwater fish culture is confronted with environmental problems owing to their high antibiotic inputs and antibiotic-resistant bacteria. This study explored the antibiotic resistance gene (ARG) profiles of water and fish gut contents from three fishponds in Tianjin using a metagenomics approach. Proteobacteria, Bacteroidetes, and Actinobacteria were the most abundant phyla in all water samples. However, the microbial composition of the fish guts was distinctly different among the three aquaculture farms. <i>Cetobacterium</i>, <i>Bacillus</i>, <i>Weissella</i>, and <i>Fusobacterium</i> were the dominant genera in the gut contents of all fish. More than 20 unique ARGs with relatively high abundances were detected in both water and fish gut content samples. The dominant genes and pathways of antibiotic resistance mechanisms detected in all samples were antibiotic efflux, antibiotic inactivation, antibiotic target alteration, antibiotic target protection, antibiotic target replacement, and reduced permeability to antibiotics. In addition, our results indicate that antibiotics, such as florfenicol, and heavy metals, such as Zn and Cu, could have a significant correlation with some common ARGs, indicating that antibiotic-resistant bacteria could co-occur with heavy metals. Our study provides a research basis for the development of a strategy for practical antibiotic application and heavy metal monitoring in aquaculture.https://www.mdpi.com/2673-9496/2/4/16antibiotic resistance genesbacterial communityantibioticheavy metalaquaculture environment |
| spellingShingle | Qiushui Wang Xin Zhou Yue Liu Qi Ding Zan Wu Jie Deng Jia Zuo Liyan Yuan Peng Shao Bo Cheng Lijuan Gao Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China Aquaculture Journal antibiotic resistance genes bacterial community antibiotic heavy metal aquaculture environment |
| title | Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China |
| title_full | Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China |
| title_fullStr | Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China |
| title_full_unstemmed | Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China |
| title_short | Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China |
| title_sort | bacterial community and antibiotic resistance gene profiles of fish gut contents and their aquaculture environment in tianjin china |
| topic | antibiotic resistance genes bacterial community antibiotic heavy metal aquaculture environment |
| url | https://www.mdpi.com/2673-9496/2/4/16 |
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