Genetic diversity of Bacillus anthracis Ames lineage strains in China
Abstract Background Anthrax is an endemic disease that persists in the rural regions of China. The global genetic population structure of B.anthracis has also been defined by the canonical single-nucleotide polymorphisms (canSNP) and multiple-locus variable-number tandem repeat analysis (MLVA). Five...
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BMC
2020-02-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-020-4867-5 |
| _version_ | 1818417634531606528 |
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| author | Enmin Zhang Huijuan Zhang Jinrong He Wei Li Jianchun Wei |
| author_facet | Enmin Zhang Huijuan Zhang Jinrong He Wei Li Jianchun Wei |
| author_sort | Enmin Zhang |
| collection | DOAJ |
| description | Abstract Background Anthrax is an endemic disease that persists in the rural regions of China. The global genetic population structure of B.anthracis has also been defined by the canonical single-nucleotide polymorphisms (canSNP) and multiple-locus variable-number tandem repeat analysis (MLVA). Five canSNP lineages were found in China, and the A.Br.Ames lineage has been the second predominant group in recent years in China. The objective of this study was to reveal genetic diversity of the Ames lineage strains by MLVA. Methods Two molecular typing methods, canSNP and MLVA with 15markers were used to study the genetic relationship among the Ames lineage strains. The outbreak information associated with these strains was also collected and investigated. Results From 2007 to 2018, a total of 21 human anthrax infection outbreaks (68 patients) associated with B. anthracis Ames lineage strains were reported in China. Ames lineage strain-associated human anthrax is mainly distributed in the northern part of China, including the provinces of Inner Mongolia, Liaoning, Gansu, and Xinjiang. In the study, a total of 30 Ames lineage strains were included and 10 MLVA15 genotypes were identified. These strains were mainly found in northeast China, Inner Mongolia and Liaoning. In recent years, the Ames lineage strains were isolated in the two provinces every year. The 18 Ames lineage strains isolated from Inner Mongolia were divided into eight MLVA15 genotypes. From 2010 to 2015, there were continuous reports of outbreaks in Keyouzhongqi County, Inner Mongolia, and the strains that were isolated annually in succession belonged to the MLVA15–30 genotype. Conclusions The Ames lineage strains are widely distributed in northern China. Their genetic diversity can be illustrated by the results of the MLVA. The genetic characteristics of the Ames lineage strains from outbreaks in different provinces varied. In some areas, human anthrax outbreaks occurred annually in succession, and these related strains grouped together. These observations indicate that the local environment was persistently contaminated with B. anthracis spores, vaccination of livestock should become the fundamental control measure in the areas. |
| first_indexed | 2024-12-14T12:09:54Z |
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| id | doaj.art-7a3685b50db941c5a72ce88fc1b2c795 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-14T12:09:54Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-7a3685b50db941c5a72ce88fc1b2c7952022-12-21T23:01:46ZengBMCBMC Infectious Diseases1471-23342020-02-012011710.1186/s12879-020-4867-5Genetic diversity of Bacillus anthracis Ames lineage strains in ChinaEnmin Zhang0Huijuan Zhang1Jinrong He2Wei Li3Jianchun Wei4National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionAbstract Background Anthrax is an endemic disease that persists in the rural regions of China. The global genetic population structure of B.anthracis has also been defined by the canonical single-nucleotide polymorphisms (canSNP) and multiple-locus variable-number tandem repeat analysis (MLVA). Five canSNP lineages were found in China, and the A.Br.Ames lineage has been the second predominant group in recent years in China. The objective of this study was to reveal genetic diversity of the Ames lineage strains by MLVA. Methods Two molecular typing methods, canSNP and MLVA with 15markers were used to study the genetic relationship among the Ames lineage strains. The outbreak information associated with these strains was also collected and investigated. Results From 2007 to 2018, a total of 21 human anthrax infection outbreaks (68 patients) associated with B. anthracis Ames lineage strains were reported in China. Ames lineage strain-associated human anthrax is mainly distributed in the northern part of China, including the provinces of Inner Mongolia, Liaoning, Gansu, and Xinjiang. In the study, a total of 30 Ames lineage strains were included and 10 MLVA15 genotypes were identified. These strains were mainly found in northeast China, Inner Mongolia and Liaoning. In recent years, the Ames lineage strains were isolated in the two provinces every year. The 18 Ames lineage strains isolated from Inner Mongolia were divided into eight MLVA15 genotypes. From 2010 to 2015, there were continuous reports of outbreaks in Keyouzhongqi County, Inner Mongolia, and the strains that were isolated annually in succession belonged to the MLVA15–30 genotype. Conclusions The Ames lineage strains are widely distributed in northern China. Their genetic diversity can be illustrated by the results of the MLVA. The genetic characteristics of the Ames lineage strains from outbreaks in different provinces varied. In some areas, human anthrax outbreaks occurred annually in succession, and these related strains grouped together. These observations indicate that the local environment was persistently contaminated with B. anthracis spores, vaccination of livestock should become the fundamental control measure in the areas.https://doi.org/10.1186/s12879-020-4867-5AnthraxBacillus anthracisAmes lineage strainscanSNPMLVA |
| spellingShingle | Enmin Zhang Huijuan Zhang Jinrong He Wei Li Jianchun Wei Genetic diversity of Bacillus anthracis Ames lineage strains in China BMC Infectious Diseases Anthrax Bacillus anthracis Ames lineage strains canSNP MLVA |
| title | Genetic diversity of Bacillus anthracis Ames lineage strains in China |
| title_full | Genetic diversity of Bacillus anthracis Ames lineage strains in China |
| title_fullStr | Genetic diversity of Bacillus anthracis Ames lineage strains in China |
| title_full_unstemmed | Genetic diversity of Bacillus anthracis Ames lineage strains in China |
| title_short | Genetic diversity of Bacillus anthracis Ames lineage strains in China |
| title_sort | genetic diversity of bacillus anthracis ames lineage strains in china |
| topic | Anthrax Bacillus anthracis Ames lineage strains canSNP MLVA |
| url | https://doi.org/10.1186/s12879-020-4867-5 |
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