Cytotaxonomy and Molecular Analyses of <i>Mycteria americana</i> (Ciconiidae: Ciconiiformes): Insights on Stork Phylogeny
Although molecular information for the wood stork (<i>Mycteria americana</i>) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversifica...
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MDPI AG
2023-03-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/4/816 |
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| author | Rodrigo Petry Corrêa de Sousa Paula Sabrina Bronze Campos Michelly da Silva dos Santos Patricia Caroline O’Brien Malcolm Andrew Ferguson-Smith Edivaldo Herculano Corrêa de Oliveira |
| author_facet | Rodrigo Petry Corrêa de Sousa Paula Sabrina Bronze Campos Michelly da Silva dos Santos Patricia Caroline O’Brien Malcolm Andrew Ferguson-Smith Edivaldo Herculano Corrêa de Oliveira |
| author_sort | Rodrigo Petry Corrêa de Sousa |
| collection | DOAJ |
| description | Although molecular information for the wood stork (<i>Mycteria americana</i>) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of <i>M. americana</i>, and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with <i>Gallus gallus</i> (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp <i>COI</i> and 1007 bp <i>Cytb</i> genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae. |
| first_indexed | 2024-03-11T05:00:10Z |
| format | Article |
| id | doaj.art-7a36d6172b4d4380ab9e303709b70fc4 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T05:00:10Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-7a36d6172b4d4380ab9e303709b70fc42023-11-17T19:22:52ZengMDPI AGGenes2073-44252023-03-0114481610.3390/genes14040816Cytotaxonomy and Molecular Analyses of <i>Mycteria americana</i> (Ciconiidae: Ciconiiformes): Insights on Stork PhylogenyRodrigo Petry Corrêa de Sousa0Paula Sabrina Bronze Campos1Michelly da Silva dos Santos2Patricia Caroline O’Brien3Malcolm Andrew Ferguson-Smith4Edivaldo Herculano Corrêa de Oliveira5Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, BrazilPrograma de Pós Graduação em Genética e Biologia Molecular, Universidade Federal do Pará,Belém 66075-110, BrazilPrograma de Pós Graduação em Genética e Biologia Molecular, Universidade Federal do Pará,Belém 66075-110, BrazilCambridge Resource Centre for Comparative Genomics, Cambridge, CB3 0ES, UKCambridge Resource Centre for Comparative Genomics, Cambridge, CB3 0ES, UKInstituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém 66075-110, BrazilAlthough molecular information for the wood stork (<i>Mycteria americana</i>) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of <i>M. americana</i>, and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with <i>Gallus gallus</i> (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp <i>COI</i> and 1007 bp <i>Cytb</i> genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae.https://www.mdpi.com/2073-4425/14/4/816woody storkchromosome painting<i>cytochrome oxidase I</i><i>cytochrome b</i> |
| spellingShingle | Rodrigo Petry Corrêa de Sousa Paula Sabrina Bronze Campos Michelly da Silva dos Santos Patricia Caroline O’Brien Malcolm Andrew Ferguson-Smith Edivaldo Herculano Corrêa de Oliveira Cytotaxonomy and Molecular Analyses of <i>Mycteria americana</i> (Ciconiidae: Ciconiiformes): Insights on Stork Phylogeny Genes woody stork chromosome painting <i>cytochrome oxidase I</i> <i>cytochrome b</i> |
| title | Cytotaxonomy and Molecular Analyses of <i>Mycteria americana</i> (Ciconiidae: Ciconiiformes): Insights on Stork Phylogeny |
| title_full | Cytotaxonomy and Molecular Analyses of <i>Mycteria americana</i> (Ciconiidae: Ciconiiformes): Insights on Stork Phylogeny |
| title_fullStr | Cytotaxonomy and Molecular Analyses of <i>Mycteria americana</i> (Ciconiidae: Ciconiiformes): Insights on Stork Phylogeny |
| title_full_unstemmed | Cytotaxonomy and Molecular Analyses of <i>Mycteria americana</i> (Ciconiidae: Ciconiiformes): Insights on Stork Phylogeny |
| title_short | Cytotaxonomy and Molecular Analyses of <i>Mycteria americana</i> (Ciconiidae: Ciconiiformes): Insights on Stork Phylogeny |
| title_sort | cytotaxonomy and molecular analyses of i mycteria americana i ciconiidae ciconiiformes insights on stork phylogeny |
| topic | woody stork chromosome painting <i>cytochrome oxidase I</i> <i>cytochrome b</i> |
| url | https://www.mdpi.com/2073-4425/14/4/816 |
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