Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with Cisplatin
The incorporation of both nitric oxide (NO) donor (S-nitrosoglutathione, GSNO) and silica nanoparticles loaded with cisplatin (SiO<sub>2</sub>@CisPt NPs) into a polymeric matrix represents a suitable approach to creating a drug-delivery system with sustained and localized drug release ag...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/14/12/2837 |
| _version_ | 1827637234083299328 |
|---|---|
| author | Bianca de Melo Santana Joana Claudio Pieretti Rafael Nunes Gomes Giselle Cerchiaro Amedea Barozzi Seabra |
| author_facet | Bianca de Melo Santana Joana Claudio Pieretti Rafael Nunes Gomes Giselle Cerchiaro Amedea Barozzi Seabra |
| author_sort | Bianca de Melo Santana |
| collection | DOAJ |
| description | The incorporation of both nitric oxide (NO) donor (S-nitrosoglutathione, GSNO) and silica nanoparticles loaded with cisplatin (SiO<sub>2</sub>@CisPt NPs) into a polymeric matrix represents a suitable approach to creating a drug-delivery system with sustained and localized drug release against tumor cells. Herein, we report the synthesis, characterization, and cytotoxicity evaluation of Pluronic F-127/hyaluronic acid hydrogel containing GSNO and SiO<sub>2</sub>@CisPt NPs against breast cancer cells. SiO<sub>2</sub>@CisPt NPs were successfully synthesized, revealing a spherical morphology with an average size of 158 ± 20 nm. Both GSNO and SiO<sub>2</sub>@CisPt NPs were incorporated into the thermoresponsive Pluronic/hyaluronic hydrogel for sustained and localized release of both NO and cisplatin. The kinetics of NO release from a hydrogel matrix revealed spontaneous and sustained release of NO at the millimolar range for 24 h. The MTT assay showed concentration-dependent cytotoxicity of the hydrogel. The combination of GSNO and SiO<sub>2</sub>@CisPt incorporated into a polymeric matrix decreased the cell viability 20% more than the hydrogel containing only GSNO or SiO<sub>2</sub>@CisPt. At 200 µg/mL, this combination led to a critical cell viability of 30%, indicating a synergistic effect between GSNO and SiO<sub>2</sub>@CisPt NPs in the hydrogel matrix, and, therefore, highlighting the potential application of this drug-delivery system in the field of biomedicine. |
| first_indexed | 2024-03-09T15:57:29Z |
| format | Article |
| id | doaj.art-7a36fe6c46464d71895be7aa13ead46b |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T15:57:29Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-7a36fe6c46464d71895be7aa13ead46b2023-11-24T17:22:52ZengMDPI AGPharmaceutics1999-49232022-12-011412283710.3390/pharmaceutics14122837Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with CisplatinBianca de Melo Santana0Joana Claudio Pieretti1Rafael Nunes Gomes2Giselle Cerchiaro3Amedea Barozzi Seabra4Center for Natural and Human Sciences, Federal University of ABC, Santo André 09210-580, BrazilCenter for Natural and Human Sciences, Federal University of ABC, Santo André 09210-580, BrazilCenter for Natural and Human Sciences, Federal University of ABC, Santo André 09210-580, BrazilCenter for Natural and Human Sciences, Federal University of ABC, Santo André 09210-580, BrazilCenter for Natural and Human Sciences, Federal University of ABC, Santo André 09210-580, BrazilThe incorporation of both nitric oxide (NO) donor (S-nitrosoglutathione, GSNO) and silica nanoparticles loaded with cisplatin (SiO<sub>2</sub>@CisPt NPs) into a polymeric matrix represents a suitable approach to creating a drug-delivery system with sustained and localized drug release against tumor cells. Herein, we report the synthesis, characterization, and cytotoxicity evaluation of Pluronic F-127/hyaluronic acid hydrogel containing GSNO and SiO<sub>2</sub>@CisPt NPs against breast cancer cells. SiO<sub>2</sub>@CisPt NPs were successfully synthesized, revealing a spherical morphology with an average size of 158 ± 20 nm. Both GSNO and SiO<sub>2</sub>@CisPt NPs were incorporated into the thermoresponsive Pluronic/hyaluronic hydrogel for sustained and localized release of both NO and cisplatin. The kinetics of NO release from a hydrogel matrix revealed spontaneous and sustained release of NO at the millimolar range for 24 h. The MTT assay showed concentration-dependent cytotoxicity of the hydrogel. The combination of GSNO and SiO<sub>2</sub>@CisPt incorporated into a polymeric matrix decreased the cell viability 20% more than the hydrogel containing only GSNO or SiO<sub>2</sub>@CisPt. At 200 µg/mL, this combination led to a critical cell viability of 30%, indicating a synergistic effect between GSNO and SiO<sub>2</sub>@CisPt NPs in the hydrogel matrix, and, therefore, highlighting the potential application of this drug-delivery system in the field of biomedicine.https://www.mdpi.com/1999-4923/14/12/2837nitric oxidenanoparticleshydrogelcytotoxicitydrug deliverybreast cancer cells |
| spellingShingle | Bianca de Melo Santana Joana Claudio Pieretti Rafael Nunes Gomes Giselle Cerchiaro Amedea Barozzi Seabra Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with Cisplatin Pharmaceutics nitric oxide nanoparticles hydrogel cytotoxicity drug delivery breast cancer cells |
| title | Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with Cisplatin |
| title_full | Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with Cisplatin |
| title_fullStr | Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with Cisplatin |
| title_full_unstemmed | Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with Cisplatin |
| title_short | Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with Cisplatin |
| title_sort | cytotoxicity towards breast cancer cells of pluronic f 127 hyaluronic acid hydrogel containing nitric oxide donor and silica nanoparticles loaded with cisplatin |
| topic | nitric oxide nanoparticles hydrogel cytotoxicity drug delivery breast cancer cells |
| url | https://www.mdpi.com/1999-4923/14/12/2837 |
| work_keys_str_mv | AT biancademelosantana cytotoxicitytowardsbreastcancercellsofpluronicf127hyaluronicacidhydrogelcontainingnitricoxidedonorandsilicananoparticlesloadedwithcisplatin AT joanaclaudiopieretti cytotoxicitytowardsbreastcancercellsofpluronicf127hyaluronicacidhydrogelcontainingnitricoxidedonorandsilicananoparticlesloadedwithcisplatin AT rafaelnunesgomes cytotoxicitytowardsbreastcancercellsofpluronicf127hyaluronicacidhydrogelcontainingnitricoxidedonorandsilicananoparticlesloadedwithcisplatin AT gisellecerchiaro cytotoxicitytowardsbreastcancercellsofpluronicf127hyaluronicacidhydrogelcontainingnitricoxidedonorandsilicananoparticlesloadedwithcisplatin AT amedeabarozziseabra cytotoxicitytowardsbreastcancercellsofpluronicf127hyaluronicacidhydrogelcontainingnitricoxidedonorandsilicananoparticlesloadedwithcisplatin |