Nobiletin enhances peroxiredoxin-1, preventing UVA radiation-induced oxidative stress and photoaging in human skin epidermal keratinocytes
This study investigates the role of nobiletin (NBN), a dietary flavonoid preventing ultraviolet-A (UVA) radiation-induced oxidative damage and photoaging responses in skin epidermal keratinocytes (HaCaT). NBN treatment prevents cell toxicity, ROS, DNA damage, and apoptosis in UVA-exposed HaCaT cells...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624000689 |
| _version_ | 1827329811444400128 |
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| author | Jianming Wei Agilan Balupillai Asaikkutti Annamalai Azhagu Pavithra Selvababu Ernest David |
| author_facet | Jianming Wei Agilan Balupillai Asaikkutti Annamalai Azhagu Pavithra Selvababu Ernest David |
| author_sort | Jianming Wei |
| collection | DOAJ |
| description | This study investigates the role of nobiletin (NBN), a dietary flavonoid preventing ultraviolet-A (UVA) radiation-induced oxidative damage and photoaging responses in skin epidermal keratinocytes (HaCaT). NBN treatment prevents cell toxicity, ROS, DNA damage, and apoptosis in UVA-exposed HaCaT cells. Furthermore, NBN prevents UVA radiation treatment-mediated overexpression of p-Erk-1, p-Jnk, p-p38, and AP-1 in HaCaT cells. Peroxiredoxin I (Prdx-1) is an antioxidant protein and enhanced activities of Prdx-1 have been considered a major target for inhibition of UVA radiation-mediated oxidative damage and photoaging responses. In this study, we noticed that NBN enhanced Prdx-1 expression, thereby enhancing antioxidants and inhibiting matrix metalloproteinase (MMP-1, MMP-2, MMP-9) and collagen degradation (Col-I and Col-III) in UVA-exposed HaCaT cells. Moreover, NBN treatment prevents UVA irradiation-induced downregulations of p-Smad and TGF-β1 expressions in HaCaT cells. In conclusion, our present results stated that NBN effectively prevents UVA-induced skin oxidative damage and photoaging responses through enhanced Prdx-1 expression. |
| first_indexed | 2024-03-07T15:47:33Z |
| format | Article |
| id | doaj.art-7a373faf03354574ab5dd65bfa68a8c5 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-07T15:47:33Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-7a373faf03354574ab5dd65bfa68a8c52024-03-05T04:29:36ZengElsevierJournal of Functional Foods1756-46462024-03-01114106066Nobiletin enhances peroxiredoxin-1, preventing UVA radiation-induced oxidative stress and photoaging in human skin epidermal keratinocytesJianming Wei0Agilan Balupillai1Asaikkutti Annamalai2Azhagu Pavithra Selvababu3Ernest David4Department of Dermatology, Fifth Medical Centre of Chinese PLA General Hospital, 100071, ChinaDepartment of Biotechnology, Thiruvalluvar University, Serkkadu-632115, Vellore, Tamil Nadu, India; Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry-605014, India; Corresponding authors.Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry-605014, IndiaDepartment of Biotechnology, Thiruvalluvar University, Serkkadu-632115, Vellore, Tamil Nadu, IndiaDepartment of Biotechnology, Thiruvalluvar University, Serkkadu-632115, Vellore, Tamil Nadu, India; Corresponding authors.This study investigates the role of nobiletin (NBN), a dietary flavonoid preventing ultraviolet-A (UVA) radiation-induced oxidative damage and photoaging responses in skin epidermal keratinocytes (HaCaT). NBN treatment prevents cell toxicity, ROS, DNA damage, and apoptosis in UVA-exposed HaCaT cells. Furthermore, NBN prevents UVA radiation treatment-mediated overexpression of p-Erk-1, p-Jnk, p-p38, and AP-1 in HaCaT cells. Peroxiredoxin I (Prdx-1) is an antioxidant protein and enhanced activities of Prdx-1 have been considered a major target for inhibition of UVA radiation-mediated oxidative damage and photoaging responses. In this study, we noticed that NBN enhanced Prdx-1 expression, thereby enhancing antioxidants and inhibiting matrix metalloproteinase (MMP-1, MMP-2, MMP-9) and collagen degradation (Col-I and Col-III) in UVA-exposed HaCaT cells. Moreover, NBN treatment prevents UVA irradiation-induced downregulations of p-Smad and TGF-β1 expressions in HaCaT cells. In conclusion, our present results stated that NBN effectively prevents UVA-induced skin oxidative damage and photoaging responses through enhanced Prdx-1 expression.http://www.sciencedirect.com/science/article/pii/S1756464624000689NobiletinUVA radiationPeroxiredoxin-1Human epidermal keratinocytes |
| spellingShingle | Jianming Wei Agilan Balupillai Asaikkutti Annamalai Azhagu Pavithra Selvababu Ernest David Nobiletin enhances peroxiredoxin-1, preventing UVA radiation-induced oxidative stress and photoaging in human skin epidermal keratinocytes Journal of Functional Foods Nobiletin UVA radiation Peroxiredoxin-1 Human epidermal keratinocytes |
| title | Nobiletin enhances peroxiredoxin-1, preventing UVA radiation-induced oxidative stress and photoaging in human skin epidermal keratinocytes |
| title_full | Nobiletin enhances peroxiredoxin-1, preventing UVA radiation-induced oxidative stress and photoaging in human skin epidermal keratinocytes |
| title_fullStr | Nobiletin enhances peroxiredoxin-1, preventing UVA radiation-induced oxidative stress and photoaging in human skin epidermal keratinocytes |
| title_full_unstemmed | Nobiletin enhances peroxiredoxin-1, preventing UVA radiation-induced oxidative stress and photoaging in human skin epidermal keratinocytes |
| title_short | Nobiletin enhances peroxiredoxin-1, preventing UVA radiation-induced oxidative stress and photoaging in human skin epidermal keratinocytes |
| title_sort | nobiletin enhances peroxiredoxin 1 preventing uva radiation induced oxidative stress and photoaging in human skin epidermal keratinocytes |
| topic | Nobiletin UVA radiation Peroxiredoxin-1 Human epidermal keratinocytes |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624000689 |
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