Cocoa Extract Indicated Has Activity on Selectively Killing Breast Cancer Cells
Effect of the cocoa crude extract on mortality of breast cancer cell lines i.e. MCF-7, T47D and normal cell (Vero), was observed. Crude cocoa extract prepared from a freshly dried cocoa bean that was containing 14% catechin and 0.6% caffeine. Catechin and caffeine content were modulated to 2-folds (...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2015-09-01
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| Series: | Journal of Tropical Life Science |
| Subjects: | |
| Online Access: | http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/290/pdf_18 |
| _version_ | 1797425413482348544 |
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| author | Ariza Budi Tunjung Sari Mahriani Gusti Agung Perias Tiningrum Teguh Wahyudi Misnawi |
| author_facet | Ariza Budi Tunjung Sari Mahriani Gusti Agung Perias Tiningrum Teguh Wahyudi Misnawi |
| author_sort | Ariza Budi Tunjung Sari |
| collection | DOAJ |
| description | Effect of the cocoa crude extract on mortality of breast cancer cell lines i.e. MCF-7, T47D and normal cell (Vero), was observed. Crude cocoa extract prepared from a freshly dried cocoa bean that was containing 14% catechin and 0.6% caffeine. Catechin and caffeine content were modulated to 2-folds (28% catechin or 1.2% caffeine) and 3-folds (42% catechin or 1.8% caffeine) by adding pure compounds. Extracts were dissolved in dimethylsulfoxide (DMSO) at concentrations ranging from 200 to 1600 μg/ml. The positive control was doxorubicin (0.5-16 μg/ml in DMSO). Cell lines (MCF-7, T47D, and Vero) were incubated in test sample for 24h at 37°, prior to 3-(4,4-dimetylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance of each well was measured at 550 nm, and lethal concentration (LC50) was calculated. The cocoa extract induced mortality of breast cancer cell lines but not in Vero cells. The effect on MCF-7 was greater than on T47D, given the LC50 was 1236 μg/ml (MCF-7) and 1893 μg/ml (T47D). Cytotoxic potential of cocoa extract was much lower than doxorubicin whose LC50 was 0,777 μg/ml (MCF-7) and 0,082 μg/ml (T47D). Increasing catechin content to 2-folds did not significantly affect LC50 value, but 3-folds catechin content reduced LC50 to 1021 μg/ml. Meanwhile increasing caffeine content to 2-folds significantly reduced LC50 to 750 μg/ml, however, 3-fold content resulted in slightly higher LC50 at 780 μg/ml. This indicates that cocoa extract have anti-cancer potential, and purification may improve this property. |
| first_indexed | 2024-03-09T08:15:48Z |
| format | Article |
| id | doaj.art-7a3b34522f5a45b4b624f00467fe1c64 |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-09T08:15:48Z |
| publishDate | 2015-09-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-7a3b34522f5a45b4b624f00467fe1c642023-12-02T22:23:47ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172015-09-0153128132Cocoa Extract Indicated Has Activity on Selectively Killing Breast Cancer CellsAriza Budi Tunjung Sari0Mahriani1Gusti Agung Perias Tiningrum2Teguh Wahyudi3Misnawi4Indonesian Coffee and Cocoa Research Institute (ICCRI), Jl. PB Sudirman 90, Jember 68118, East Java, Indonesia, www.iccri.net, iccri@iccri.netDepartment of Biology, Faculty of Mathematics and Sciences, Jember University, Jl. Kalimantan 37, Jember 68121, East Java, IndonesiaDepartment of Biology, Faculty of Mathematics and Sciences, Jember University, Jl. Kalimantan 37, Jember 68121, East Java, IndonesiaIndonesian Coffee and Cocoa Research Institute (ICCRI), Jl. PB Sudirman 90, Jember 68118, East Java, Indonesia, www.iccri.net, iccri@iccri.netIndonesian Coffee and Cocoa Research Institute (ICCRI), Jl. PB Sudirman 90, Jember 68118, East Java, Indonesia, www.iccri.net, iccri@iccri.netEffect of the cocoa crude extract on mortality of breast cancer cell lines i.e. MCF-7, T47D and normal cell (Vero), was observed. Crude cocoa extract prepared from a freshly dried cocoa bean that was containing 14% catechin and 0.6% caffeine. Catechin and caffeine content were modulated to 2-folds (28% catechin or 1.2% caffeine) and 3-folds (42% catechin or 1.8% caffeine) by adding pure compounds. Extracts were dissolved in dimethylsulfoxide (DMSO) at concentrations ranging from 200 to 1600 μg/ml. The positive control was doxorubicin (0.5-16 μg/ml in DMSO). Cell lines (MCF-7, T47D, and Vero) were incubated in test sample for 24h at 37°, prior to 3-(4,4-dimetylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance of each well was measured at 550 nm, and lethal concentration (LC50) was calculated. The cocoa extract induced mortality of breast cancer cell lines but not in Vero cells. The effect on MCF-7 was greater than on T47D, given the LC50 was 1236 μg/ml (MCF-7) and 1893 μg/ml (T47D). Cytotoxic potential of cocoa extract was much lower than doxorubicin whose LC50 was 0,777 μg/ml (MCF-7) and 0,082 μg/ml (T47D). Increasing catechin content to 2-folds did not significantly affect LC50 value, but 3-folds catechin content reduced LC50 to 1021 μg/ml. Meanwhile increasing caffeine content to 2-folds significantly reduced LC50 to 750 μg/ml, however, 3-fold content resulted in slightly higher LC50 at 780 μg/ml. This indicates that cocoa extract have anti-cancer potential, and purification may improve this property.http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/290/pdf_18breast cancercocoacytotoxicityMCF-7T47D |
| spellingShingle | Ariza Budi Tunjung Sari Mahriani Gusti Agung Perias Tiningrum Teguh Wahyudi Misnawi Cocoa Extract Indicated Has Activity on Selectively Killing Breast Cancer Cells Journal of Tropical Life Science breast cancer cocoa cytotoxicity MCF-7 T47D |
| title | Cocoa Extract Indicated Has Activity on Selectively Killing Breast Cancer Cells |
| title_full | Cocoa Extract Indicated Has Activity on Selectively Killing Breast Cancer Cells |
| title_fullStr | Cocoa Extract Indicated Has Activity on Selectively Killing Breast Cancer Cells |
| title_full_unstemmed | Cocoa Extract Indicated Has Activity on Selectively Killing Breast Cancer Cells |
| title_short | Cocoa Extract Indicated Has Activity on Selectively Killing Breast Cancer Cells |
| title_sort | cocoa extract indicated has activity on selectively killing breast cancer cells |
| topic | breast cancer cocoa cytotoxicity MCF-7 T47D |
| url | http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/290/pdf_18 |
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