Molecular and behavioral consequences of Ube3a gene overdosage in mice
Chromosome 15q11.2–q13.1 duplication syndrome (Dup15q syndrome) is a severe neurodevelopmental disorder characterized by intellectual disability, impaired motor coordination, and autism spectrum disorder. Chromosomal multiplication of the UBE3A gene is presumed to be the primary driver of Dup15q pat...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Clinical investigation
2022-09-01
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| Series: | JCI Insight |
| Subjects: | |
| Online Access: | https://doi.org/10.1172/jci.insight.158953 |
| _version_ | 1797634370221113344 |
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| author | A. Mattijs Punt Matthew C. Judson Michael S. Sidorov Brittany N. Williams Naomi S. Johnson Sabine Belder Dion den Hertog Courtney R. Davis Maximillian S. Feygin Patrick F. Lang Mehrnoush Aghadavoud Jolfaei Patrick J. Curran Wilfred F.J. van IJcken Ype Elgersma Benjamin D. Philpot |
| author_facet | A. Mattijs Punt Matthew C. Judson Michael S. Sidorov Brittany N. Williams Naomi S. Johnson Sabine Belder Dion den Hertog Courtney R. Davis Maximillian S. Feygin Patrick F. Lang Mehrnoush Aghadavoud Jolfaei Patrick J. Curran Wilfred F.J. van IJcken Ype Elgersma Benjamin D. Philpot |
| author_sort | A. Mattijs Punt |
| collection | DOAJ |
| description | Chromosome 15q11.2–q13.1 duplication syndrome (Dup15q syndrome) is a severe neurodevelopmental disorder characterized by intellectual disability, impaired motor coordination, and autism spectrum disorder. Chromosomal multiplication of the UBE3A gene is presumed to be the primary driver of Dup15q pathophysiology, given that UBE3A exhibits maternal monoallelic expression in neurons and that maternal duplications typically yield far more severe neurodevelopmental outcomes than paternal duplications. However, studies into the pathogenic effects of UBE3A overexpression in mice have yielded conflicting results. Here, we investigated the neurodevelopmental impact of Ube3a gene overdosage using bacterial artificial chromosome–based transgenic mouse models (Ube3aOE) that recapitulate the increases in Ube3a copy number most often observed in Dup15q. In contrast to previously published Ube3a overexpression models, Ube3aOE mice were indistinguishable from wild-type controls on a number of molecular and behavioral measures, despite suffering increased mortality when challenged with seizures, a phenotype reminiscent of sudden unexpected death in epilepsy. Collectively, our data support a model wherein pathogenic synergy between UBE3A and other overexpressed 15q11.2–q13.1 genes is required for full penetrance of Dup15q syndrome phenotypes. |
| first_indexed | 2024-03-11T12:07:46Z |
| format | Article |
| id | doaj.art-7a3cbddaed7a4251860363085a8801d7 |
| institution | Directory Open Access Journal |
| issn | 2379-3708 |
| language | English |
| last_indexed | 2024-03-11T12:07:46Z |
| publishDate | 2022-09-01 |
| publisher | American Society for Clinical investigation |
| record_format | Article |
| series | JCI Insight |
| spelling | doaj.art-7a3cbddaed7a4251860363085a8801d72023-11-07T16:24:35ZengAmerican Society for Clinical investigationJCI Insight2379-37082022-09-01718Molecular and behavioral consequences of Ube3a gene overdosage in miceA. Mattijs PuntMatthew C. JudsonMichael S. SidorovBrittany N. WilliamsNaomi S. JohnsonSabine BelderDion den HertogCourtney R. DavisMaximillian S. FeyginPatrick F. LangMehrnoush Aghadavoud JolfaeiPatrick J. CurranWilfred F.J. van IJckenYpe ElgersmaBenjamin D. PhilpotChromosome 15q11.2–q13.1 duplication syndrome (Dup15q syndrome) is a severe neurodevelopmental disorder characterized by intellectual disability, impaired motor coordination, and autism spectrum disorder. Chromosomal multiplication of the UBE3A gene is presumed to be the primary driver of Dup15q pathophysiology, given that UBE3A exhibits maternal monoallelic expression in neurons and that maternal duplications typically yield far more severe neurodevelopmental outcomes than paternal duplications. However, studies into the pathogenic effects of UBE3A overexpression in mice have yielded conflicting results. Here, we investigated the neurodevelopmental impact of Ube3a gene overdosage using bacterial artificial chromosome–based transgenic mouse models (Ube3aOE) that recapitulate the increases in Ube3a copy number most often observed in Dup15q. In contrast to previously published Ube3a overexpression models, Ube3aOE mice were indistinguishable from wild-type controls on a number of molecular and behavioral measures, despite suffering increased mortality when challenged with seizures, a phenotype reminiscent of sudden unexpected death in epilepsy. Collectively, our data support a model wherein pathogenic synergy between UBE3A and other overexpressed 15q11.2–q13.1 genes is required for full penetrance of Dup15q syndrome phenotypes.https://doi.org/10.1172/jci.insight.158953Neuroscience |
| spellingShingle | A. Mattijs Punt Matthew C. Judson Michael S. Sidorov Brittany N. Williams Naomi S. Johnson Sabine Belder Dion den Hertog Courtney R. Davis Maximillian S. Feygin Patrick F. Lang Mehrnoush Aghadavoud Jolfaei Patrick J. Curran Wilfred F.J. van IJcken Ype Elgersma Benjamin D. Philpot Molecular and behavioral consequences of Ube3a gene overdosage in mice JCI Insight Neuroscience |
| title | Molecular and behavioral consequences of Ube3a gene overdosage in mice |
| title_full | Molecular and behavioral consequences of Ube3a gene overdosage in mice |
| title_fullStr | Molecular and behavioral consequences of Ube3a gene overdosage in mice |
| title_full_unstemmed | Molecular and behavioral consequences of Ube3a gene overdosage in mice |
| title_short | Molecular and behavioral consequences of Ube3a gene overdosage in mice |
| title_sort | molecular and behavioral consequences of ube3a gene overdosage in mice |
| topic | Neuroscience |
| url | https://doi.org/10.1172/jci.insight.158953 |
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