Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease
Background: Current data on the possible involvement of aging neutrophils in atherogenesis are limited. This study aimed to research the diagnostic value of aging neutrophils in their relation to subclinical atherosclerosis in statin-naïve patients without established atherosclerotic cardiovascular...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/17/10195 |
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| author | Vadim Genkel Ilya Dolgushin Irina Baturina Albina Savochkina Karina Nikushkina Anna Minasova Lubov Pykhova Veronika Sumerkina Alla Kuznetsova Igor Shaposhnik |
| author_facet | Vadim Genkel Ilya Dolgushin Irina Baturina Albina Savochkina Karina Nikushkina Anna Minasova Lubov Pykhova Veronika Sumerkina Alla Kuznetsova Igor Shaposhnik |
| author_sort | Vadim Genkel |
| collection | DOAJ |
| description | Background: Current data on the possible involvement of aging neutrophils in atherogenesis are limited. This study aimed to research the diagnostic value of aging neutrophils in their relation to subclinical atherosclerosis in statin-naïve patients without established atherosclerotic cardiovascular diseases (ASCVD). Methods: The study was carried out on 151 statin-naïve patients aged 40–64 years old without ASCVD. All patients underwent duplex scanning of the carotid arteries, lower limb arteries and abdominal aorta. Phenotyping and differentiation of neutrophil subpopulations were performed through flow cytometry (Navios 6/2, Beckman Coulter, USA). Results: The number of CD62L<sup>lo</sup>CXCR4<sup>hi</sup>-neutrophils is known to be significantly higher in patients with subclinical atherosclerosis compared with patients without atherosclerosis (<i>p</i> = 0.006). An increase in the number of CD62L<sup>lo</sup>CXCR4<sup>hi</sup>-neutrophils above cut-off values makes it possible to predict atherosclerosis in at least one vascular bed with sensitivity of 35.4–50.5% and specificity of 80.0–92.1%, in two vascular beds with sensitivity of 44.7–84.4% and specificity of 80.8–33.3%. Conclusion: In statin-naïve patients 40–64 years old without established ASCVD with subclinical atherosclerosis, there is an increase in circulating CD62L<sup>lo</sup>CXCR4<sup>hi</sup>-neutrophils. It was also concluded that the increase in the number of circulating CD62L<sup>lo</sup>CXCR4<sup>hi</sup>-neutrophils demonstrated moderate diagnostic efficiency (AUC 0.617–0.656) in relation to the detection of subclinical atherosclerosis, including polyvascular atherosclerosis. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:40:25Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7a3f82c3c592437ebc4216ad8d8b4c952023-11-23T13:24:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123171019510.3390/ijms231710195Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular DiseaseVadim Genkel0Ilya Dolgushin1Irina Baturina2Albina Savochkina3Karina Nikushkina4Anna Minasova5Lubov Pykhova6Veronika Sumerkina7Alla Kuznetsova8Igor Shaposhnik9Federal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaFederal State Budgetary Educational Institution of Higher Education “South-Ural State Medical University” of the Ministry of Healthcare of the Russian Federation, 454092 Chelyabinsk, RussiaBackground: Current data on the possible involvement of aging neutrophils in atherogenesis are limited. This study aimed to research the diagnostic value of aging neutrophils in their relation to subclinical atherosclerosis in statin-naïve patients without established atherosclerotic cardiovascular diseases (ASCVD). Methods: The study was carried out on 151 statin-naïve patients aged 40–64 years old without ASCVD. All patients underwent duplex scanning of the carotid arteries, lower limb arteries and abdominal aorta. Phenotyping and differentiation of neutrophil subpopulations were performed through flow cytometry (Navios 6/2, Beckman Coulter, USA). Results: The number of CD62L<sup>lo</sup>CXCR4<sup>hi</sup>-neutrophils is known to be significantly higher in patients with subclinical atherosclerosis compared with patients without atherosclerosis (<i>p</i> = 0.006). An increase in the number of CD62L<sup>lo</sup>CXCR4<sup>hi</sup>-neutrophils above cut-off values makes it possible to predict atherosclerosis in at least one vascular bed with sensitivity of 35.4–50.5% and specificity of 80.0–92.1%, in two vascular beds with sensitivity of 44.7–84.4% and specificity of 80.8–33.3%. Conclusion: In statin-naïve patients 40–64 years old without established ASCVD with subclinical atherosclerosis, there is an increase in circulating CD62L<sup>lo</sup>CXCR4<sup>hi</sup>-neutrophils. It was also concluded that the increase in the number of circulating CD62L<sup>lo</sup>CXCR4<sup>hi</sup>-neutrophils demonstrated moderate diagnostic efficiency (AUC 0.617–0.656) in relation to the detection of subclinical atherosclerosis, including polyvascular atherosclerosis.https://www.mdpi.com/1422-0067/23/17/10195atherosclerosisneutrophilsinflammationaging neutrophilsCXCL12/CXCR4 axis |
| spellingShingle | Vadim Genkel Ilya Dolgushin Irina Baturina Albina Savochkina Karina Nikushkina Anna Minasova Lubov Pykhova Veronika Sumerkina Alla Kuznetsova Igor Shaposhnik Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease International Journal of Molecular Sciences atherosclerosis neutrophils inflammation aging neutrophils CXCL12/CXCR4 axis |
| title | Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease |
| title_full | Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease |
| title_fullStr | Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease |
| title_full_unstemmed | Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease |
| title_short | Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease |
| title_sort | circulating ageing neutrophils as a marker of asymptomatic polyvascular atherosclerosis in statin naive patients without established cardiovascular disease |
| topic | atherosclerosis neutrophils inflammation aging neutrophils CXCL12/CXCR4 axis |
| url | https://www.mdpi.com/1422-0067/23/17/10195 |
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