Maximum Entropy Method-Based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor–Acceptor Distance Distribution: Theory and Simulation
A maximum entropy method-based acceptor triplet-state fluorescence correlation spectroscopy (tsFCS) analysis, i.e., tsFCS fluorescence resonance energy transfer (tsFCS-FRET), was proposed to resolve the donor-acceptor distance (R) distribution of a FRET system with multiple distances. An R-dependent...
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| Format: | Article |
| Language: | English |
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IEEE
2015-01-01
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| Series: | IEEE Photonics Journal |
| Subjects: | |
| Online Access: | https://ieeexplore.ieee.org/document/7069259/ |
| _version_ | 1818457903150923776 |
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| author | B. L. Chen Z. Y. Guo T. S. Chen |
| author_facet | B. L. Chen Z. Y. Guo T. S. Chen |
| author_sort | B. L. Chen |
| collection | DOAJ |
| description | A maximum entropy method-based acceptor triplet-state fluorescence correlation spectroscopy (tsFCS) analysis, i.e., tsFCS fluorescence resonance energy transfer (tsFCS-FRET), was proposed to resolve the donor-acceptor distance (R) distribution of a FRET system with multiple distances. An R-dependent acceptor triplet-state weight distribution function was introduced into the excited tsFCS model with a fixed R, and the weight distribution maximized the value of the Shannon entropy. tsFCS-FRET analysis showed consistent distributions with the actual pre-input for both unimodal distribution and two-species systems and a distribution with three peaks close to the pre-inputted values for three-species system at higher donor laser power. Collectively, tsFCS-FRET provides a powerful tool for resolving the R distribution, in turn, to the FRET efficiency (E) distribution, of a FRET system containing multiple E species. |
| first_indexed | 2024-12-14T22:49:57Z |
| format | Article |
| id | doaj.art-7a3fe141bb1e44749bbac2620f783643 |
| institution | Directory Open Access Journal |
| issn | 1943-0655 |
| language | English |
| last_indexed | 2024-12-14T22:49:57Z |
| publishDate | 2015-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Photonics Journal |
| spelling | doaj.art-7a3fe141bb1e44749bbac2620f7836432022-12-21T22:44:45ZengIEEEIEEE Photonics Journal1943-06552015-01-017311010.1109/JPHOT.2015.24171687069259Maximum Entropy Method-Based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor–Acceptor Distance Distribution: Theory and SimulationB. L. Chen0Z. Y. Guo1T. S. Chen2MOE Key Lab. of Laser Life Sci., South China Normal Univ., Guangzhou, ChinaMOE Key Lab. of Laser Life Sci., South China Normal Univ., Guangzhou, ChinaMOE Key Lab. of Laser Life Sci., South China Normal Univ., Guangzhou, ChinaA maximum entropy method-based acceptor triplet-state fluorescence correlation spectroscopy (tsFCS) analysis, i.e., tsFCS fluorescence resonance energy transfer (tsFCS-FRET), was proposed to resolve the donor-acceptor distance (R) distribution of a FRET system with multiple distances. An R-dependent acceptor triplet-state weight distribution function was introduced into the excited tsFCS model with a fixed R, and the weight distribution maximized the value of the Shannon entropy. tsFCS-FRET analysis showed consistent distributions with the actual pre-input for both unimodal distribution and two-species systems and a distribution with three peaks close to the pre-inputted values for three-species system at higher donor laser power. Collectively, tsFCS-FRET provides a powerful tool for resolving the R distribution, in turn, to the FRET efficiency (E) distribution, of a FRET system containing multiple E species.https://ieeexplore.ieee.org/document/7069259/fluorescence resonance energy transfer (FRET)FRET efficiency (E)auto-correlation (AC)MemExp |
| spellingShingle | B. L. Chen Z. Y. Guo T. S. Chen Maximum Entropy Method-Based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor–Acceptor Distance Distribution: Theory and Simulation IEEE Photonics Journal fluorescence resonance energy transfer (FRET) FRET efficiency (E) auto-correlation (AC) MemExp |
| title | Maximum Entropy Method-Based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor–Acceptor Distance Distribution: Theory and Simulation |
| title_full | Maximum Entropy Method-Based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor–Acceptor Distance Distribution: Theory and Simulation |
| title_fullStr | Maximum Entropy Method-Based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor–Acceptor Distance Distribution: Theory and Simulation |
| title_full_unstemmed | Maximum Entropy Method-Based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor–Acceptor Distance Distribution: Theory and Simulation |
| title_short | Maximum Entropy Method-Based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor–Acceptor Distance Distribution: Theory and Simulation |
| title_sort | maximum entropy method based acceptor triplet state fluorescence correlation spectroscopy analysis for determination of donor x2013 acceptor distance distribution theory and simulation |
| topic | fluorescence resonance energy transfer (FRET) FRET efficiency (E) auto-correlation (AC) MemExp |
| url | https://ieeexplore.ieee.org/document/7069259/ |
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