Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles
As an essential genetic and economic trait, chicken feather color has long been an important research topic. To further understand the mechanism of melanin deposition associated with coloration in chicken feathers, we selected feather follicle tissues from the neck and wings of chickens with differe...
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MDPI AG
2023-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/16/2608 |
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| author | Ruiting Li Yanxing Wang Yihan Liu Donghua Li Yadong Tian Xiaojun Liu Xiangtao Kang Zhuanjian Li |
| author_facet | Ruiting Li Yanxing Wang Yihan Liu Donghua Li Yadong Tian Xiaojun Liu Xiangtao Kang Zhuanjian Li |
| author_sort | Ruiting Li |
| collection | DOAJ |
| description | As an essential genetic and economic trait, chicken feather color has long been an important research topic. To further understand the mechanism of melanin deposition associated with coloration in chicken feathers, we selected feather follicle tissues from the neck and wings of chickens with differently colored feathers (yellow, sub-Columbian, and silver) for transcriptome analysis. We focused on genes that were expressed in both the wings and neck and were expressed with the same trends in breeds with two different plumage colors, specifically, <i>SLC45A2</i>, <i>GPNMB</i>, <i>MLPH</i>, <i>TYR</i>, <i>KIT</i>, <i>WNT11</i>, and <i>FZD1</i>. GO and KEGG enrichment analyses showed the DEGs were enriched in melanin-related pathways, such as tyrosine metabolic pathway and melanogenesis, and PPI analysis highlighted the genes <i>SLC45A2</i> and <i>GPNMB</i> as associated with melanin deposition. Verification experiments in chicken melanocytes demonstrated that these two genes promote melanocyte melanin deposition. These data enrich our knowledge of the mechanisms that regulate chicken feather color. |
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| id | doaj.art-7a42756ec8094a8688df406485a623e3 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T00:11:35Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj.art-7a42756ec8094a8688df406485a623e32023-11-18T23:57:38ZengMDPI AGAnimals2076-26152023-08-011316260810.3390/ani13162608Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather FolliclesRuiting Li0Yanxing Wang1Yihan Liu2Donghua Li3Yadong Tian4Xiaojun Liu5Xiangtao Kang6Zhuanjian Li7College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaAs an essential genetic and economic trait, chicken feather color has long been an important research topic. To further understand the mechanism of melanin deposition associated with coloration in chicken feathers, we selected feather follicle tissues from the neck and wings of chickens with differently colored feathers (yellow, sub-Columbian, and silver) for transcriptome analysis. We focused on genes that were expressed in both the wings and neck and were expressed with the same trends in breeds with two different plumage colors, specifically, <i>SLC45A2</i>, <i>GPNMB</i>, <i>MLPH</i>, <i>TYR</i>, <i>KIT</i>, <i>WNT11</i>, and <i>FZD1</i>. GO and KEGG enrichment analyses showed the DEGs were enriched in melanin-related pathways, such as tyrosine metabolic pathway and melanogenesis, and PPI analysis highlighted the genes <i>SLC45A2</i> and <i>GPNMB</i> as associated with melanin deposition. Verification experiments in chicken melanocytes demonstrated that these two genes promote melanocyte melanin deposition. These data enrich our knowledge of the mechanisms that regulate chicken feather color.https://www.mdpi.com/2076-2615/13/16/2608transcriptomemelaninmelanocytes<i>SLC45A2</i><i>GPNMB</i> |
| spellingShingle | Ruiting Li Yanxing Wang Yihan Liu Donghua Li Yadong Tian Xiaojun Liu Xiangtao Kang Zhuanjian Li Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles Animals transcriptome melanin melanocytes <i>SLC45A2</i> <i>GPNMB</i> |
| title | Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles |
| title_full | Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles |
| title_fullStr | Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles |
| title_full_unstemmed | Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles |
| title_short | Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles |
| title_sort | effects of slc45a2 and gpnmb on melanin deposition based on transcriptome sequencing in chicken feather follicles |
| topic | transcriptome melanin melanocytes <i>SLC45A2</i> <i>GPNMB</i> |
| url | https://www.mdpi.com/2076-2615/13/16/2608 |
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