Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's Disease
Background: Parkinson's Disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta of the mid brain which leads to the depletion of dopamine levels. The depletion of dopamine leads to the motor dysfunction characterized by tremors, rigidity, bradykin...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031321001135 |
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| author | Yasir Hasan Siddique Falaq Naz Rahul Mohammad Rashid Shariq Mian |
| author_facet | Yasir Hasan Siddique Falaq Naz Rahul Mohammad Rashid Shariq Mian |
| author_sort | Yasir Hasan Siddique |
| collection | DOAJ |
| description | Background: Parkinson's Disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta of the mid brain which leads to the depletion of dopamine levels. The depletion of dopamine leads to the motor dysfunction characterized by tremors, rigidity, bradykinesia and postural instability. In this context, the effect of unani herbal formulation Itrifal Muqawwi-e-Dimagh (IMD) was studied on the transgenic Drosophila melanogaster expressing human alpha synuclein in the neurons of flies. IMD is recommended for enhancing mental power and to treat mental illness in the Unani system of medicine. IMD was prepared as per the standard procedures and was subjected to various physico-chemical tests. Methods: The equivalents of recommended dose for human were established for 20 g of fly food i.e. 0.0014, 0.0028, 0.0042 and 0.0056 g per 20 g of diet. The PD flies were allowed to feed on it for 24 days before performing the assays. Results: The PD flies exposed to IMD showed increase in the life span and improvement in the activity. A significant dose dependent delay in the loss of climbing ability was observed in PD flies exposed to various doses of IMD. The exposure of PD flies to IMD showed a significant reduction in the oxidative stress and increase in the anti-oxidant enzymes compared to unexposed PD flies. The PD flies exposed to various doses of IMD showed a significant dose dependent increase in the activity of tyrosine hydroxylase as evident by immunohistochemistry. Conclusion: It is concluded that IMD is potent in reducing the PD symptoms being mimicked in the transgenic Drosophila. |
| first_indexed | 2024-12-15T00:37:59Z |
| format | Article |
| id | doaj.art-7a42cbf185e849adaba39c2b0d778840 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-12-15T00:37:59Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-7a42cbf185e849adaba39c2b0d7788402022-12-21T22:41:44ZengElsevierPhytomedicine Plus2667-03132021-11-0114100131Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's DiseaseYasir Hasan Siddique0Falaq Naz1 Rahul2Mohammad Rashid3Shariq Mian4Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India; Corresponding author.Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, IndiaDrosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, IndiaDepartment of Saidla, Ajmal khan Tibbiya College, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, IndiaDepartment of Saidla, Ajmal khan Tibbiya College, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, IndiaBackground: Parkinson's Disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta of the mid brain which leads to the depletion of dopamine levels. The depletion of dopamine leads to the motor dysfunction characterized by tremors, rigidity, bradykinesia and postural instability. In this context, the effect of unani herbal formulation Itrifal Muqawwi-e-Dimagh (IMD) was studied on the transgenic Drosophila melanogaster expressing human alpha synuclein in the neurons of flies. IMD is recommended for enhancing mental power and to treat mental illness in the Unani system of medicine. IMD was prepared as per the standard procedures and was subjected to various physico-chemical tests. Methods: The equivalents of recommended dose for human were established for 20 g of fly food i.e. 0.0014, 0.0028, 0.0042 and 0.0056 g per 20 g of diet. The PD flies were allowed to feed on it for 24 days before performing the assays. Results: The PD flies exposed to IMD showed increase in the life span and improvement in the activity. A significant dose dependent delay in the loss of climbing ability was observed in PD flies exposed to various doses of IMD. The exposure of PD flies to IMD showed a significant reduction in the oxidative stress and increase in the anti-oxidant enzymes compared to unexposed PD flies. The PD flies exposed to various doses of IMD showed a significant dose dependent increase in the activity of tyrosine hydroxylase as evident by immunohistochemistry. Conclusion: It is concluded that IMD is potent in reducing the PD symptoms being mimicked in the transgenic Drosophila.http://www.sciencedirect.com/science/article/pii/S2667031321001135Parkinson's DiseaseItrifal Muqawwi-e-DimaghDrosophila melanogasterOxidative stress |
| spellingShingle | Yasir Hasan Siddique Falaq Naz Rahul Mohammad Rashid Shariq Mian Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's Disease Phytomedicine Plus Parkinson's Disease Itrifal Muqawwi-e-Dimagh Drosophila melanogaster Oxidative stress |
| title | Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's Disease |
| title_full | Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's Disease |
| title_fullStr | Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's Disease |
| title_full_unstemmed | Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's Disease |
| title_short | Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's Disease |
| title_sort | effect of itrifal muqawwi e dimagh a polyherbal drug on the transgenic drosophila model of parkinson s disease |
| topic | Parkinson's Disease Itrifal Muqawwi-e-Dimagh Drosophila melanogaster Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2667031321001135 |
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