Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement
Occurrence of d-amino acids in living organisms is a useful indicator of various changes, diseases, or disorders. Determination of amino acid enantiomers, namely the enantiomeric ratio of amino acids or excess of certain d-amino acids, represents a useful tool in the studies of aging processes or bi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-10-01
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| Series: | Separations |
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| Online Access: | http://www.mdpi.com/2297-8739/3/4/30 |
| _version_ | 1798025431687888896 |
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| author | Květa Kalíková Tereza Šlechtová Eva Tesařová |
| author_facet | Květa Kalíková Tereza Šlechtová Eva Tesařová |
| author_sort | Květa Kalíková |
| collection | DOAJ |
| description | Occurrence of d-amino acids in living organisms is a useful indicator of various changes, diseases, or disorders. Determination of amino acid enantiomers, namely the enantiomeric ratio of amino acids or excess of certain d-amino acids, represents a useful tool in the studies of aging processes or biomarkers in disease/disorder diagnosis in humans. The amount of d-amino acids is usually very low. Therefore, suitable sample pretreatment, often derivatization, and highly selective and sensitive separation methods are essential for d-amino acid analysis in this field. Chromatographic techniques offer appropriate choices for solving these tasks. This review covers the advances in methodology and development of improved instrumental chromatographic methods focused on d,l-amino acid separation and determination. New findings in the area of possible d-amino acid biomarkers are also included. |
| first_indexed | 2024-04-11T18:18:58Z |
| format | Article |
| id | doaj.art-7a444266044c4fbc8727660ac44ce85f |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-04-11T18:18:58Z |
| publishDate | 2016-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-7a444266044c4fbc8727660ac44ce85f2022-12-22T04:09:49ZengMDPI AGSeparations2297-87392016-10-01343010.3390/separations3040030separations3040030Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic MeasurementKvěta Kalíková0Tereza Šlechtová1Eva Tesařová2Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, 128 43 Prague, Czech RepublicDepartment of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, 128 43 Prague, Czech RepublicDepartment of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, 128 43 Prague, Czech RepublicOccurrence of d-amino acids in living organisms is a useful indicator of various changes, diseases, or disorders. Determination of amino acid enantiomers, namely the enantiomeric ratio of amino acids or excess of certain d-amino acids, represents a useful tool in the studies of aging processes or biomarkers in disease/disorder diagnosis in humans. The amount of d-amino acids is usually very low. Therefore, suitable sample pretreatment, often derivatization, and highly selective and sensitive separation methods are essential for d-amino acid analysis in this field. Chromatographic techniques offer appropriate choices for solving these tasks. This review covers the advances in methodology and development of improved instrumental chromatographic methods focused on d,l-amino acid separation and determination. New findings in the area of possible d-amino acid biomarkers are also included.http://www.mdpi.com/2297-8739/3/4/30chromatographyamino acidsenantiomeric ratioagingbiomarkers |
| spellingShingle | Květa Kalíková Tereza Šlechtová Eva Tesařová Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement Separations chromatography amino acids enantiomeric ratio aging biomarkers |
| title | Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement |
| title_full | Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement |
| title_fullStr | Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement |
| title_full_unstemmed | Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement |
| title_short | Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement |
| title_sort | enantiomeric ratio of amino acids as a tool for determination of aging and disease diagnostics by chromatographic measurement |
| topic | chromatography amino acids enantiomeric ratio aging biomarkers |
| url | http://www.mdpi.com/2297-8739/3/4/30 |
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