MTRR rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin, but not with congenital heart disease or coronary atherosclerosis in Brazilian patients
Background: Differences in the distribution of the MTRR rs326119 polymorphism (c.56+781 A>C) between patients with congenital heart disease (CHD) and controls have been described in Chinese individuals. The association is thought to be due to deregulation of homocysteine-cobalamin pathways. This...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | International Journal of Cardiology: Heart & Vasculature |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352906716300884 |
| _version_ | 1818318352823615488 |
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| author | Melanie Horita Carolina Tosin Bueno Andrea R Horimoto Pedro A Lemos Antonio A Morandini-Filho Jose E Krieger Paulo C J L Santos Alexandre C Pereira |
| author_facet | Melanie Horita Carolina Tosin Bueno Andrea R Horimoto Pedro A Lemos Antonio A Morandini-Filho Jose E Krieger Paulo C J L Santos Alexandre C Pereira |
| author_sort | Melanie Horita |
| collection | DOAJ |
| description | Background: Differences in the distribution of the MTRR rs326119 polymorphism (c.56+781 A>C) between patients with congenital heart disease (CHD) and controls have been described in Chinese individuals. The association is thought to be due to deregulation of homocysteine-cobalamin pathways. This has not been replicated in other populations. The primary objective of this study was to assess the influence of the MTRR rs326119 polymorphism on biochemical parameters of vitamin B12 metabolism, coronary lesions, and congenital heart disease in Brazilian subjects.
Methods: We selected 722 patients with CHD, 1432 patients who underwent coronary angiography, and 156 blood donors. Genotyping for the MTRR polymorphism was evaluated by high-resolution melting analysis, and biochemical tests of vitamin B12 metabolism were measured.
Results: Subjects carrying the AC or CC genotypes had higher homocysteine concentrations (9.7 ± 0.4 μmol/L and 10.1 ± 0.6 μmol/L) and lower cobalamin concentrations (260.5 ± 13.3 pmol/L and 275.6 ± 19.9 pmol/L) compared with the subjects carrying the AA genotype (8.7 ± 0.5 μmol/L and 304.8 ± 14.7 pmol/L), respectively. A multiple linear regression model also identified a significant association between the number of C variant alleles with the concentrations of homocysteine and cobalamin. Nonetheless, the allelic and genotypic distributions for MTRR rs326119 were not associated with CHD or coronary atherosclerosis in the studied samples.
Conclusion: Our findings indicate that the MTRR rs326119 variant might be a genetic marker associated with homocysteine and cobalamin concentrations, but not a strong risk factor for CHD or coronary atherosclerosis in the Brazilian population. |
| first_indexed | 2024-12-13T09:51:52Z |
| format | Article |
| id | doaj.art-7a452ea1b046471cbab30179c02ee38c |
| institution | Directory Open Access Journal |
| issn | 2352-9067 |
| language | English |
| last_indexed | 2024-12-13T09:51:52Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Cardiology: Heart & Vasculature |
| spelling | doaj.art-7a452ea1b046471cbab30179c02ee38c2022-12-21T23:51:54ZengElsevierInternational Journal of Cardiology: Heart & Vasculature2352-90672017-03-0114C1510.1016/j.ijcha.2016.11.004MTRR rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin, but not with congenital heart disease or coronary atherosclerosis in Brazilian patientsMelanie Horita0Carolina Tosin Bueno1Andrea R Horimoto2Pedro A Lemos3Antonio A Morandini-Filho4Jose E Krieger5Paulo C J L Santos6Alexandre C Pereira7Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of São Paulo Medical School, BrazilLaboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of São Paulo Medical School, BrazilLaboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of São Paulo Medical School, BrazilHemodynamic Laboratory, Heart Institute (InCor), University of São Paulo Medical School, BrazilLaboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of São Paulo Medical School, BrazilLaboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of São Paulo Medical School, BrazilLaboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of São Paulo Medical School, BrazilLaboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of São Paulo Medical School, BrazilBackground: Differences in the distribution of the MTRR rs326119 polymorphism (c.56+781 A>C) between patients with congenital heart disease (CHD) and controls have been described in Chinese individuals. The association is thought to be due to deregulation of homocysteine-cobalamin pathways. This has not been replicated in other populations. The primary objective of this study was to assess the influence of the MTRR rs326119 polymorphism on biochemical parameters of vitamin B12 metabolism, coronary lesions, and congenital heart disease in Brazilian subjects. Methods: We selected 722 patients with CHD, 1432 patients who underwent coronary angiography, and 156 blood donors. Genotyping for the MTRR polymorphism was evaluated by high-resolution melting analysis, and biochemical tests of vitamin B12 metabolism were measured. Results: Subjects carrying the AC or CC genotypes had higher homocysteine concentrations (9.7 ± 0.4 μmol/L and 10.1 ± 0.6 μmol/L) and lower cobalamin concentrations (260.5 ± 13.3 pmol/L and 275.6 ± 19.9 pmol/L) compared with the subjects carrying the AA genotype (8.7 ± 0.5 μmol/L and 304.8 ± 14.7 pmol/L), respectively. A multiple linear regression model also identified a significant association between the number of C variant alleles with the concentrations of homocysteine and cobalamin. Nonetheless, the allelic and genotypic distributions for MTRR rs326119 were not associated with CHD or coronary atherosclerosis in the studied samples. Conclusion: Our findings indicate that the MTRR rs326119 variant might be a genetic marker associated with homocysteine and cobalamin concentrations, but not a strong risk factor for CHD or coronary atherosclerosis in the Brazilian population.http://www.sciencedirect.com/science/article/pii/S2352906716300884MTRR geners326119Congenital heart diseaseHomocysteineCobalamin |
| spellingShingle | Melanie Horita Carolina Tosin Bueno Andrea R Horimoto Pedro A Lemos Antonio A Morandini-Filho Jose E Krieger Paulo C J L Santos Alexandre C Pereira MTRR rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin, but not with congenital heart disease or coronary atherosclerosis in Brazilian patients International Journal of Cardiology: Heart & Vasculature MTRR gene rs326119 Congenital heart disease Homocysteine Cobalamin |
| title | MTRR rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin, but not with congenital heart disease or coronary atherosclerosis in Brazilian patients |
| title_full | MTRR rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin, but not with congenital heart disease or coronary atherosclerosis in Brazilian patients |
| title_fullStr | MTRR rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin, but not with congenital heart disease or coronary atherosclerosis in Brazilian patients |
| title_full_unstemmed | MTRR rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin, but not with congenital heart disease or coronary atherosclerosis in Brazilian patients |
| title_short | MTRR rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin, but not with congenital heart disease or coronary atherosclerosis in Brazilian patients |
| title_sort | mtrr rs326119 polymorphism is associated with plasma concentrations of homocysteine and cobalamin but not with congenital heart disease or coronary atherosclerosis in brazilian patients |
| topic | MTRR gene rs326119 Congenital heart disease Homocysteine Cobalamin |
| url | http://www.sciencedirect.com/science/article/pii/S2352906716300884 |
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