Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models—Introduction of the “RatOx” Oxygenator and Preliminary In Vitro Results
The overall survival rate of extracorporeal life support (ECLS) remains at 60%. Research and development has been slow, in part due to the lack of sophisticated experimental models. This publication introduces a dedicated rodent oxygenator (“RatOx”) and presents preliminary in vitro classification t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/4/800 |
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| author | Lasse J. Strudthoff Jannis Focke Felix Hesselmann Andreas Kaesler Ana Martins Costa Peter C. Schlanstein Thomas Schmitz-Rode Ulrich Steinseifer Niklas B. Steuer Bettina Wiegmann Jutta Arens Sebastian V. Jansen |
| author_facet | Lasse J. Strudthoff Jannis Focke Felix Hesselmann Andreas Kaesler Ana Martins Costa Peter C. Schlanstein Thomas Schmitz-Rode Ulrich Steinseifer Niklas B. Steuer Bettina Wiegmann Jutta Arens Sebastian V. Jansen |
| author_sort | Lasse J. Strudthoff |
| collection | DOAJ |
| description | The overall survival rate of extracorporeal life support (ECLS) remains at 60%. Research and development has been slow, in part due to the lack of sophisticated experimental models. This publication introduces a dedicated rodent oxygenator (“RatOx”) and presents preliminary in vitro classification tests. The RatOx has an adaptable fiber module size for various rodent models. Gas transfer performances over the fiber module for different blood flows and fiber module sizes were tested according to DIN EN ISO 7199. At the maximum possible amount of effective fiber surface area and a blood flow of 100 mL/min, the oxygenator performance was tested to a maximum of 6.27 mL O<sub>2</sub>/min and 8.2 mL CO<sub>2</sub>/min, respectively. The priming volume for the largest fiber module is 5.4 mL, while the smallest possible configuration with a single fiber mat layer has a priming volume of 1.1 mL. The novel RatOx ECLS system has been evaluated in vitro and has demonstrated a high degree of compliance with all pre-defined functional criteria for rodent-sized animal models. We intend for the RatOx to become a standard testing platform for scientific studies on ECLS therapy and technology. |
| first_indexed | 2024-03-11T04:44:42Z |
| format | Article |
| id | doaj.art-7a47cea3c73d4b6b80dab036f29125cd |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T04:44:42Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-7a47cea3c73d4b6b80dab036f29125cd2023-11-17T20:29:32ZengMDPI AGMicromachines2072-666X2023-03-0114480010.3390/mi14040800Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models—Introduction of the “RatOx” Oxygenator and Preliminary In Vitro ResultsLasse J. Strudthoff0Jannis Focke1Felix Hesselmann2Andreas Kaesler3Ana Martins Costa4Peter C. Schlanstein5Thomas Schmitz-Rode6Ulrich Steinseifer7Niklas B. Steuer8Bettina Wiegmann9Jutta Arens10Sebastian V. Jansen11Institute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyInstitute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyInstitute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyInstitute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Biomechanical Engineering, Faculty of Engineering Technologies, University of Twente, 7522 LW Enschede, The NetherlandsInstitute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyInstitute of Applied Medical Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyInstitute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyInstitute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyDepartment for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hanover, GermanyInstitute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyInstitute of Applied Medical Engineering, Department of Cardiovascular Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyThe overall survival rate of extracorporeal life support (ECLS) remains at 60%. Research and development has been slow, in part due to the lack of sophisticated experimental models. This publication introduces a dedicated rodent oxygenator (“RatOx”) and presents preliminary in vitro classification tests. The RatOx has an adaptable fiber module size for various rodent models. Gas transfer performances over the fiber module for different blood flows and fiber module sizes were tested according to DIN EN ISO 7199. At the maximum possible amount of effective fiber surface area and a blood flow of 100 mL/min, the oxygenator performance was tested to a maximum of 6.27 mL O<sub>2</sub>/min and 8.2 mL CO<sub>2</sub>/min, respectively. The priming volume for the largest fiber module is 5.4 mL, while the smallest possible configuration with a single fiber mat layer has a priming volume of 1.1 mL. The novel RatOx ECLS system has been evaluated in vitro and has demonstrated a high degree of compliance with all pre-defined functional criteria for rodent-sized animal models. We intend for the RatOx to become a standard testing platform for scientific studies on ECLS therapy and technology.https://www.mdpi.com/2072-666X/14/4/800ECLSECMOanimal modelrodentratmouse |
| spellingShingle | Lasse J. Strudthoff Jannis Focke Felix Hesselmann Andreas Kaesler Ana Martins Costa Peter C. Schlanstein Thomas Schmitz-Rode Ulrich Steinseifer Niklas B. Steuer Bettina Wiegmann Jutta Arens Sebastian V. Jansen Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models—Introduction of the “RatOx” Oxygenator and Preliminary In Vitro Results Micromachines ECLS ECMO animal model rodent rat mouse |
| title | Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models—Introduction of the “RatOx” Oxygenator and Preliminary In Vitro Results |
| title_full | Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models—Introduction of the “RatOx” Oxygenator and Preliminary In Vitro Results |
| title_fullStr | Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models—Introduction of the “RatOx” Oxygenator and Preliminary In Vitro Results |
| title_full_unstemmed | Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models—Introduction of the “RatOx” Oxygenator and Preliminary In Vitro Results |
| title_short | Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models—Introduction of the “RatOx” Oxygenator and Preliminary In Vitro Results |
| title_sort | novel size variable dedicated rodent oxygenator for ecls animal models introduction of the ratox oxygenator and preliminary in vitro results |
| topic | ECLS ECMO animal model rodent rat mouse |
| url | https://www.mdpi.com/2072-666X/14/4/800 |
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