Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection
Abstract Clostridioides difficile is the leading cause of antibiotic-associated infectious diarrhea. The development of C.difficile infection is tied to perturbations of the bacterial community in the gastrointestinal tract, called the gastrointestinal microbiota. Repairing the gastrointestinal micr...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-05778-6 |
| _version_ | 1827329020460531712 |
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| author | Katya Douchant Shu-Mei He Curtis Noordhof Jill Greenlaw Sarah Vancuren Kathleen Schroeter Emma Allen-Vercoe Calvin Sjaarda Stephen J. Vanner Elaine O. Petrof Prameet M. Sheth Mabel Guzman |
| author_facet | Katya Douchant Shu-Mei He Curtis Noordhof Jill Greenlaw Sarah Vancuren Kathleen Schroeter Emma Allen-Vercoe Calvin Sjaarda Stephen J. Vanner Elaine O. Petrof Prameet M. Sheth Mabel Guzman |
| author_sort | Katya Douchant |
| collection | DOAJ |
| description | Abstract Clostridioides difficile is the leading cause of antibiotic-associated infectious diarrhea. The development of C.difficile infection is tied to perturbations of the bacterial community in the gastrointestinal tract, called the gastrointestinal microbiota. Repairing the gastrointestinal microbiota by introducing lab-designed bacterial communities, or defined microbial communities, has recently shown promise as therapeutics against C.difficile infection, however, the mechanisms of action of defined microbial communities remain unclear. Using an antibiotic- C.difficile mouse model, we report the ability of an 18-member community and a refined 4-member community to protect mice from two ribotypes of C.difficile (CD027, CD078; p < 0.05). Furthermore, bacteria-free supernatant delivered orally to mice from the 4-member community proteolyzed C.difficile toxins in vitro and protected mice from C.difficile infection in vivo (p < 0.05). This study demonstrates that bacteria-free supernatant is sufficient to protect mice from C.difficile; and could be further explored as a therapeutic strategy against C.difficile infection. |
| first_indexed | 2024-03-07T15:27:50Z |
| format | Article |
| id | doaj.art-7a4b2c5a7efa4d30a08a1140befa2ea5 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-07T15:27:50Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-7a4b2c5a7efa4d30a08a1140befa2ea52024-03-05T16:38:43ZengNature PortfolioCommunications Biology2399-36422024-01-017111210.1038/s42003-024-05778-6Defined microbial communities and their soluble products protect mice from Clostridioides difficile infectionKatya Douchant0Shu-Mei He1Curtis Noordhof2Jill Greenlaw3Sarah Vancuren4Kathleen Schroeter5Emma Allen-Vercoe6Calvin Sjaarda7Stephen J. Vanner8Elaine O. Petrof9Prameet M. Sheth10Mabel Guzman11The Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterThe Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterThe Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterThe Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterDepartment of Molecular and Cellular Biology, University of GuelphDepartment of Molecular and Cellular Biology, University of GuelphDepartment of Molecular and Cellular Biology, University of GuelphThe Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterThe Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterThe Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterThe Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterThe Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences CenterAbstract Clostridioides difficile is the leading cause of antibiotic-associated infectious diarrhea. The development of C.difficile infection is tied to perturbations of the bacterial community in the gastrointestinal tract, called the gastrointestinal microbiota. Repairing the gastrointestinal microbiota by introducing lab-designed bacterial communities, or defined microbial communities, has recently shown promise as therapeutics against C.difficile infection, however, the mechanisms of action of defined microbial communities remain unclear. Using an antibiotic- C.difficile mouse model, we report the ability of an 18-member community and a refined 4-member community to protect mice from two ribotypes of C.difficile (CD027, CD078; p < 0.05). Furthermore, bacteria-free supernatant delivered orally to mice from the 4-member community proteolyzed C.difficile toxins in vitro and protected mice from C.difficile infection in vivo (p < 0.05). This study demonstrates that bacteria-free supernatant is sufficient to protect mice from C.difficile; and could be further explored as a therapeutic strategy against C.difficile infection.https://doi.org/10.1038/s42003-024-05778-6 |
| spellingShingle | Katya Douchant Shu-Mei He Curtis Noordhof Jill Greenlaw Sarah Vancuren Kathleen Schroeter Emma Allen-Vercoe Calvin Sjaarda Stephen J. Vanner Elaine O. Petrof Prameet M. Sheth Mabel Guzman Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection Communications Biology |
| title | Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection |
| title_full | Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection |
| title_fullStr | Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection |
| title_full_unstemmed | Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection |
| title_short | Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection |
| title_sort | defined microbial communities and their soluble products protect mice from clostridioides difficile infection |
| url | https://doi.org/10.1038/s42003-024-05778-6 |
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