Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles
Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimicrobial potential, low toxicity to humans, high thermal stability, biocompatibility, and low cost of production. However, their successful transformation into sustainable drugs is limited due to their lo...
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MDPI AG
2023-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/2/112 |
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| author | Ashapurna Khatua Kajal Kumari Deepak Khatak Annesha Roy Neelima Bhatt Bernard Paul Aparupa Naik Amiya Kumar Patel Uttam Kumar Panigrahi Santosh Kumar Sahu Muthupandian Saravanan Ramovatar Meena |
| author_facet | Ashapurna Khatua Kajal Kumari Deepak Khatak Annesha Roy Neelima Bhatt Bernard Paul Aparupa Naik Amiya Kumar Patel Uttam Kumar Panigrahi Santosh Kumar Sahu Muthupandian Saravanan Ramovatar Meena |
| author_sort | Ashapurna Khatua |
| collection | DOAJ |
| description | Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimicrobial potential, low toxicity to humans, high thermal stability, biocompatibility, and low cost of production. However, their successful transformation into sustainable drugs is limited due to their low membrane permeability, which reduces their bioavailability in target cells. Herein we propose Cerium-doped magnesium oxide nanoparticles (MgOCeNPs) as a powerful solution to above mentioned limitations and are compared with MgO NPs for their membrane permeability and antimicrobial activity. Both pure and Ce-doped were characterized by various spectroscopic and microscopic techniques, in which an X-ray diffraction (XRD) examination reveals the lattice patterns for doped nanoparticles. Furthermore, Atomic Force Microscopy (AFM) revealed the three-dimensional (3D) structure and height of the nanoparticle. The crystal structure (FCC) of MgO did not change with Ce doping. However, microstructural properties like lattice parameter, crystallite size and biological activity of MgO significantly changed with Ce doping. In order to evaluate the antimicrobial potential of MgOCeNPs in comparison to MgO NPs and to understand the underlying mechanisms, the antibacterial activity was investigated against human pathogenic bacteria <i>E. coli</i> and <i>P. aeruginosa,</i> and antifungal activity against THY-1, a fungal strain. MgOCeNPs were studied by several methods, which resulted in a strong antibacterial and antifungal activity in the form of an elevated zone of inhibition, reduced growth curve, lower minimum inhibitory concentration (MIC<sub>80</sub>) and enhanced cytotoxicity in both bacterial and fungal strain as compared to MgO nanoparticles. The study of the growth curve showed early and prolonged stationary phase and early decline log phase. Both bacterial and fungal strains showed dose-dependent cytotoxicity with enhancement in intracellular reactive oxygen species (ROS) generation and formation of pores in the membrane when interacting with egg-phosphatidylcholine model Large Unilamellar Vesicles (LUVs). The proposed mechanism of MgOCeNPs toxicity evidently is membranolytic activity and induction of ROS production, which may cause oxidative stress-mediated cytotoxicity. These results confirmed that MgOCeNPs are a novel and very potent antimicrobial agent with a great promise of controlling and treating other microbes. |
| first_indexed | 2024-03-11T08:36:36Z |
| format | Article |
| id | doaj.art-7a4b75890d9f476c83a6c16f685cee35 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T08:36:36Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-7a4b75890d9f476c83a6c16f685cee352023-11-16T21:24:49ZengMDPI AGJournal of Functional Biomaterials2079-49832023-02-0114211210.3390/jfb14020112Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar VesiclesAshapurna Khatua0Kajal Kumari1Deepak Khatak2Annesha Roy3Neelima Bhatt4Bernard Paul5Aparupa Naik6Amiya Kumar Patel7Uttam Kumar Panigrahi8Santosh Kumar Sahu9Muthupandian Saravanan10Ramovatar Meena11School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaA.I. Virtanen Institute for Molecular Sciences, Univerisity of Eastern Finland, 70211 Kuopio, FinlandSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaInstitut Jules Guyot, University of Bourgogne, 21000 Dijon, FranceSchool of Biotechnology and Bioinformatics, Sambalpur University, Odisha 757003, IndiaSchool of Biotechnology and Bioinformatics, Sambalpur University, Odisha 757003, IndiaDepartment of Physics, Maharaja Sriram Chandra Bhanja Deo University, Odisha 757003, IndiaDepartment of Physics, Maharaja Sriram Chandra Bhanja Deo University, Odisha 757003, IndiaAMR and Nanotherapeutics Lab, Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai 600077, IndiaSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaConsiderable attention has been given to Magnesium oxide nanoparticles lately due to their antimicrobial potential, low toxicity to humans, high thermal stability, biocompatibility, and low cost of production. However, their successful transformation into sustainable drugs is limited due to their low membrane permeability, which reduces their bioavailability in target cells. Herein we propose Cerium-doped magnesium oxide nanoparticles (MgOCeNPs) as a powerful solution to above mentioned limitations and are compared with MgO NPs for their membrane permeability and antimicrobial activity. Both pure and Ce-doped were characterized by various spectroscopic and microscopic techniques, in which an X-ray diffraction (XRD) examination reveals the lattice patterns for doped nanoparticles. Furthermore, Atomic Force Microscopy (AFM) revealed the three-dimensional (3D) structure and height of the nanoparticle. The crystal structure (FCC) of MgO did not change with Ce doping. However, microstructural properties like lattice parameter, crystallite size and biological activity of MgO significantly changed with Ce doping. In order to evaluate the antimicrobial potential of MgOCeNPs in comparison to MgO NPs and to understand the underlying mechanisms, the antibacterial activity was investigated against human pathogenic bacteria <i>E. coli</i> and <i>P. aeruginosa,</i> and antifungal activity against THY-1, a fungal strain. MgOCeNPs were studied by several methods, which resulted in a strong antibacterial and antifungal activity in the form of an elevated zone of inhibition, reduced growth curve, lower minimum inhibitory concentration (MIC<sub>80</sub>) and enhanced cytotoxicity in both bacterial and fungal strain as compared to MgO nanoparticles. The study of the growth curve showed early and prolonged stationary phase and early decline log phase. Both bacterial and fungal strains showed dose-dependent cytotoxicity with enhancement in intracellular reactive oxygen species (ROS) generation and formation of pores in the membrane when interacting with egg-phosphatidylcholine model Large Unilamellar Vesicles (LUVs). The proposed mechanism of MgOCeNPs toxicity evidently is membranolytic activity and induction of ROS production, which may cause oxidative stress-mediated cytotoxicity. These results confirmed that MgOCeNPs are a novel and very potent antimicrobial agent with a great promise of controlling and treating other microbes.https://www.mdpi.com/2079-4983/14/2/112cerium doped in magnesium oxide (MgOCeNPs)antimicrobial activitymembrane permeabilityreactive oxygen species |
| spellingShingle | Ashapurna Khatua Kajal Kumari Deepak Khatak Annesha Roy Neelima Bhatt Bernard Paul Aparupa Naik Amiya Kumar Patel Uttam Kumar Panigrahi Santosh Kumar Sahu Muthupandian Saravanan Ramovatar Meena Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles Journal of Functional Biomaterials cerium doped in magnesium oxide (MgOCeNPs) antimicrobial activity membrane permeability reactive oxygen species |
| title | Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles |
| title_full | Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles |
| title_fullStr | Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles |
| title_full_unstemmed | Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles |
| title_short | Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles |
| title_sort | synthesis and spectral characterisation of fabricated cerium doped magnesium oxide nanoparticles evaluation of the antimicrobial potential and its membranolytic activity through large unilamellar vesicles |
| topic | cerium doped in magnesium oxide (MgOCeNPs) antimicrobial activity membrane permeability reactive oxygen species |
| url | https://www.mdpi.com/2079-4983/14/2/112 |
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