Application of CRISPR/Cas9 for Rapid Genome Editing of Pseudorabies Virus and Bovine Herpesvirus-1

The CRISPR/Cas9 system is widely used to manipulate viral genomes. Although <i>Alphaherpesvirinae</i> genomes are large and complicated to edit, in recent years several Pseudorabies virus (PRV) mutants have been successfully generated using the CRISPR/Cas9 system. However, the applicatio...

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Bibliographic Details
Main Authors: Wanqi Yu, Jingyi Liu, Yingnan Liu, Maria Forlenza, Hongjun Chen
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Viruses
Subjects:
Online Access:https://www.mdpi.com/1999-4915/16/2/311