A built-in strategy to mitigate transgene spreading from genetically modified corn.
Transgene spreading is a major concern in cultivating genetically modified (GM) corn. Cross-pollination may cause the spread of transgenes from GM cornfields to conventional fields. Occasionally, seed lot contamination, volunteers, mixing during sowing, harvest, and trade can also lead to transgene...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3855706?pdf=render |
| _version_ | 1818493858493759488 |
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| author | Jing Li Hui Yu Fengzhen Zhang Chaoyang Lin Jianhua Gao Jun Fang Xiahui Ding Zhicheng Shen Xiaoli Xu |
| author_facet | Jing Li Hui Yu Fengzhen Zhang Chaoyang Lin Jianhua Gao Jun Fang Xiahui Ding Zhicheng Shen Xiaoli Xu |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Transgene spreading is a major concern in cultivating genetically modified (GM) corn. Cross-pollination may cause the spread of transgenes from GM cornfields to conventional fields. Occasionally, seed lot contamination, volunteers, mixing during sowing, harvest, and trade can also lead to transgene escape. Obviously, new biological confinement technologies are highly desired to mitigate transgene spreading in addition to physical separation and isolation methods. In this study, we report the development of a built-in containment method to mitigate transgene spreading in corn. In this method, an RNAi cassette for suppressing the expression of the nicosulfuron detoxifying enzyme CYP81A9 and an expression cassette for the glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene G10 were constructed and transformed into corn via Agrobacterium-mediated transformation. The GM corn plants that were generated were found to be sensitive to nicosulfuron but resistant to glyphosate, which is exactly the opposite of conventional corn. Field tests demonstrated that GM corn plants with silenced CYP81A9 could be killed by applying nicosulfuron at 40 g/ha, which is the recommended dose for weed control in cornfields. This study suggests that this built-in containment method for controlling the spread of corn transgenes is effective and easy to implement. |
| first_indexed | 2024-12-10T17:59:23Z |
| format | Article |
| id | doaj.art-7a4d408a8e964c32babe43b7ef21fde3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T17:59:23Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7a4d408a8e964c32babe43b7ef21fde32022-12-22T01:38:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8164510.1371/journal.pone.0081645A built-in strategy to mitigate transgene spreading from genetically modified corn.Jing LiHui YuFengzhen ZhangChaoyang LinJianhua GaoJun FangXiahui DingZhicheng ShenXiaoli XuTransgene spreading is a major concern in cultivating genetically modified (GM) corn. Cross-pollination may cause the spread of transgenes from GM cornfields to conventional fields. Occasionally, seed lot contamination, volunteers, mixing during sowing, harvest, and trade can also lead to transgene escape. Obviously, new biological confinement technologies are highly desired to mitigate transgene spreading in addition to physical separation and isolation methods. In this study, we report the development of a built-in containment method to mitigate transgene spreading in corn. In this method, an RNAi cassette for suppressing the expression of the nicosulfuron detoxifying enzyme CYP81A9 and an expression cassette for the glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene G10 were constructed and transformed into corn via Agrobacterium-mediated transformation. The GM corn plants that were generated were found to be sensitive to nicosulfuron but resistant to glyphosate, which is exactly the opposite of conventional corn. Field tests demonstrated that GM corn plants with silenced CYP81A9 could be killed by applying nicosulfuron at 40 g/ha, which is the recommended dose for weed control in cornfields. This study suggests that this built-in containment method for controlling the spread of corn transgenes is effective and easy to implement.http://europepmc.org/articles/PMC3855706?pdf=render |
| spellingShingle | Jing Li Hui Yu Fengzhen Zhang Chaoyang Lin Jianhua Gao Jun Fang Xiahui Ding Zhicheng Shen Xiaoli Xu A built-in strategy to mitigate transgene spreading from genetically modified corn. PLoS ONE |
| title | A built-in strategy to mitigate transgene spreading from genetically modified corn. |
| title_full | A built-in strategy to mitigate transgene spreading from genetically modified corn. |
| title_fullStr | A built-in strategy to mitigate transgene spreading from genetically modified corn. |
| title_full_unstemmed | A built-in strategy to mitigate transgene spreading from genetically modified corn. |
| title_short | A built-in strategy to mitigate transgene spreading from genetically modified corn. |
| title_sort | built in strategy to mitigate transgene spreading from genetically modified corn |
| url | http://europepmc.org/articles/PMC3855706?pdf=render |
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