Isolation of Cellulolytic Bacteria and Characterization of the Enzyme
Four of cellulolitic bacteria isolates had beencharacterized. The determination of cellulase activity was conducted at the highest production time, using crudeenzymes with the modification of Miller methods (1959) on pure cellulose substrates such as CMC (Carboxymethylcellulose), Avicel and Filter p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2009-04-01
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| Series: | Makara Seri Sains |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/science/article/view/369 |
| _version_ | 1819161906056790016 |
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| author | Nisa Rachmania Titi Candra Sunarti Besty Maranatha Wahyu Widosari Anja Meryandini Hasrul Satria |
| author_facet | Nisa Rachmania Titi Candra Sunarti Besty Maranatha Wahyu Widosari Anja Meryandini Hasrul Satria |
| author_sort | Nisa Rachmania |
| collection | DOAJ |
| description | Four of cellulolitic bacteria isolates had beencharacterized. The determination of cellulase activity was conducted at the highest production time, using crudeenzymes with the modification of Miller methods (1959) on pure cellulose substrates such as CMC (Carboxymethylcellulose), Avicel and Filter paper Whatman No. 1 as well as agriculture waste such as rice straw, corn cob and bananapeel. Cellulase from C4-4, C5-1, C5-3 and C11-1 showed optimum activity at pH 5, 70°C, pH 3.5, 90°C, pH 5, 80°Cand pH 8, 70°C, respectively. Avicel is a appropriate substrate for C4-4 cellulase whereas CMC for the other three.C11-1 cellulase has the highest cellulase enzyme activity on rice straw substrate whereas C4-4 cellulase on banana peelsubstrates. C5-1 and C5-3 cellulase have relatively low cellulase activities in degrading substrates of agriculture waste.However, isolates of C5-1 and C5-3 have high cellulase activities on banana peel substrates. |
| first_indexed | 2024-12-22T17:19:47Z |
| format | Article |
| id | doaj.art-7a503dc43ffb4d4c9cdce83d48ea8e2a |
| institution | Directory Open Access Journal |
| issn | 1693-6671 |
| language | English |
| last_indexed | 2024-12-22T17:19:47Z |
| publishDate | 2009-04-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Seri Sains |
| spelling | doaj.art-7a503dc43ffb4d4c9cdce83d48ea8e2a2022-12-21T18:18:52ZengUniversitas IndonesiaMakara Seri Sains1693-66712009-04-011313338Isolation of Cellulolytic Bacteria and Characterization of the EnzymeNisa RachmaniaTiti Candra SunartiBesty MaranathaWahyu WidosariAnja MeryandiniHasrul SatriaFour of cellulolitic bacteria isolates had beencharacterized. The determination of cellulase activity was conducted at the highest production time, using crudeenzymes with the modification of Miller methods (1959) on pure cellulose substrates such as CMC (Carboxymethylcellulose), Avicel and Filter paper Whatman No. 1 as well as agriculture waste such as rice straw, corn cob and bananapeel. Cellulase from C4-4, C5-1, C5-3 and C11-1 showed optimum activity at pH 5, 70°C, pH 3.5, 90°C, pH 5, 80°Cand pH 8, 70°C, respectively. Avicel is a appropriate substrate for C4-4 cellulase whereas CMC for the other three.C11-1 cellulase has the highest cellulase enzyme activity on rice straw substrate whereas C4-4 cellulase on banana peelsubstrates. C5-1 and C5-3 cellulase have relatively low cellulase activities in degrading substrates of agriculture waste.However, isolates of C5-1 and C5-3 have high cellulase activities on banana peel substrates.http://journal.ui.ac.id/science/article/view/369bacteriacelluloliticcellulaseagriculture waste |
| spellingShingle | Nisa Rachmania Titi Candra Sunarti Besty Maranatha Wahyu Widosari Anja Meryandini Hasrul Satria Isolation of Cellulolytic Bacteria and Characterization of the Enzyme Makara Seri Sains bacteria cellulolitic cellulase agriculture waste |
| title | Isolation of Cellulolytic Bacteria and Characterization of the Enzyme |
| title_full | Isolation of Cellulolytic Bacteria and Characterization of the Enzyme |
| title_fullStr | Isolation of Cellulolytic Bacteria and Characterization of the Enzyme |
| title_full_unstemmed | Isolation of Cellulolytic Bacteria and Characterization of the Enzyme |
| title_short | Isolation of Cellulolytic Bacteria and Characterization of the Enzyme |
| title_sort | isolation of cellulolytic bacteria and characterization of the enzyme |
| topic | bacteria cellulolitic cellulase agriculture waste |
| url | http://journal.ui.ac.id/science/article/view/369 |
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