The effect of carbohydrates on the adherence of Pasteurella multocida to the nasal respiratory epithelium
Abstract Pasteurella multocida subsp. multocida is responsible for different diseases that generate great economic losses in farm animal. The effectiveness of immunization against those bacteria are variable and the use of antibiotics is questioned; for that reason, we investigated the potential inh...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2021-07-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300909&tlng=en |
| _version_ | 1798021858567651328 |
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| author | CAROLINA GALLEGO PILAR PATIÑO NHORA MARTÍNEZ CARLOS IREGUI |
| author_facet | CAROLINA GALLEGO PILAR PATIÑO NHORA MARTÍNEZ CARLOS IREGUI |
| author_sort | CAROLINA GALLEGO |
| collection | DOAJ |
| description | Abstract Pasteurella multocida subsp. multocida is responsible for different diseases that generate great economic losses in farm animal. The effectiveness of immunization against those bacteria are variable and the use of antibiotics is questioned; for that reason, we investigated the potential inhibitory effect of different carbohydrates on the adherence in vivo of P. multocida to the rabbit respiratory epithelium as an alternative for the prevention of respiratory infections. Rabbits were intranasally and intratracheally inoculated with a solution containing 200 µl of 1x107 CFU of P. multocida that was previously mixed with 250 µg /200 µl of N-acetylglucosamine, alphamethylglucoside, alphamethylmannoside, N-acetylgalactosamine or sialic acid. The animals that received N-acetylglucosamine, alphamethylglucoside or alphamethylmannoside individually or a mixture of these three carbohydrates plus the bacterium, showed a significant decrease (P <0.05) of the clinical symptoms, microscopic and macroscopic lesions in the nasal septa and in the lungs; also, the number of adhered bacteria to the nasal epithelium were also significantly reduced. This research demonstrates for the first time that such an approach could convert into a method for prevention of P. multocida infection in rabbits that is ecologically and economically safe and effective. |
| first_indexed | 2024-04-11T17:20:15Z |
| format | Article |
| id | doaj.art-7a5205b3c91d40cda0208a98ec3acd48 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T17:20:15Z |
| publishDate | 2021-07-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-7a5205b3c91d40cda0208a98ec3acd482022-12-22T04:12:30ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-07-0193210.1590/0001-3765202120190989The effect of carbohydrates on the adherence of Pasteurella multocida to the nasal respiratory epitheliumCAROLINA GALLEGOhttps://orcid.org/0000-0001-7744-2585PILAR PATIÑOhttps://orcid.org/0000-0001-9833-601XNHORA MARTÍNEZhttps://orcid.org/0000-0001-9552-4218CARLOS IREGUIhttps://orcid.org/0000-0003-0790-8305Abstract Pasteurella multocida subsp. multocida is responsible for different diseases that generate great economic losses in farm animal. The effectiveness of immunization against those bacteria are variable and the use of antibiotics is questioned; for that reason, we investigated the potential inhibitory effect of different carbohydrates on the adherence in vivo of P. multocida to the rabbit respiratory epithelium as an alternative for the prevention of respiratory infections. Rabbits were intranasally and intratracheally inoculated with a solution containing 200 µl of 1x107 CFU of P. multocida that was previously mixed with 250 µg /200 µl of N-acetylglucosamine, alphamethylglucoside, alphamethylmannoside, N-acetylgalactosamine or sialic acid. The animals that received N-acetylglucosamine, alphamethylglucoside or alphamethylmannoside individually or a mixture of these three carbohydrates plus the bacterium, showed a significant decrease (P <0.05) of the clinical symptoms, microscopic and macroscopic lesions in the nasal septa and in the lungs; also, the number of adhered bacteria to the nasal epithelium were also significantly reduced. This research demonstrates for the first time that such an approach could convert into a method for prevention of P. multocida infection in rabbits that is ecologically and economically safe and effective.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300909&tlng=enAdherencecarbohydrateslesionsPasteurella multocida |
| spellingShingle | CAROLINA GALLEGO PILAR PATIÑO NHORA MARTÍNEZ CARLOS IREGUI The effect of carbohydrates on the adherence of Pasteurella multocida to the nasal respiratory epithelium Anais da Academia Brasileira de Ciências Adherence carbohydrates lesions Pasteurella multocida |
| title | The effect of carbohydrates on the adherence of Pasteurella multocida to the nasal respiratory epithelium |
| title_full | The effect of carbohydrates on the adherence of Pasteurella multocida to the nasal respiratory epithelium |
| title_fullStr | The effect of carbohydrates on the adherence of Pasteurella multocida to the nasal respiratory epithelium |
| title_full_unstemmed | The effect of carbohydrates on the adherence of Pasteurella multocida to the nasal respiratory epithelium |
| title_short | The effect of carbohydrates on the adherence of Pasteurella multocida to the nasal respiratory epithelium |
| title_sort | effect of carbohydrates on the adherence of pasteurella multocida to the nasal respiratory epithelium |
| topic | Adherence carbohydrates lesions Pasteurella multocida |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300909&tlng=en |
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