The Protective Effect of Selenium Nanoparticles in Osteoarthritis: In vitro and in vivo Studies
Yong Li,1,2,* Senbo Zhu,2,* Junchao Luo,2 Yu Tong,2 Yixuan Zheng,1 Lichen Ji,2 Zeju He,2 Qiangan Jing,1 Jiaqing Huang,2 Yinjun Zhang,1 Qing Bi2 1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, People’s Republic of China; 2La...
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Dove Medical Press
2023-05-01
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| Series: | Drug Design, Development and Therapy |
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| Online Access: | https://www.dovepress.com/the-protective-effect-of-selenium-nanoparticles-in-osteoarthritis-in-v-peer-reviewed-fulltext-article-DDDT |
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| author | Li Y Zhu S Luo J Tong Y Zheng Y Ji L He Z Jing Q Huang J Zhang Y Bi Q |
| author_facet | Li Y Zhu S Luo J Tong Y Zheng Y Ji L He Z Jing Q Huang J Zhang Y Bi Q |
| author_sort | Li Y |
| collection | DOAJ |
| description | Yong Li,1,2,* Senbo Zhu,2,* Junchao Luo,2 Yu Tong,2 Yixuan Zheng,1 Lichen Ji,2 Zeju He,2 Qiangan Jing,1 Jiaqing Huang,2 Yinjun Zhang,1 Qing Bi2 1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, People’s Republic of China; 2Laboratory Medicine Center, Department of Laboratory Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, 310014, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yinjun Zhang, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, People’s Republic of China, Tel +86-13735494556, Email zhangyj@zjut.edu.cn Qing Bi, Laboratory Medicine Center, Department of Laboratory Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, 310014, People’s Republic of China, Tel +86-13588302991, Email bqzjsrmyy@163.comIntroduction: Osteoarthritis (OA) is a common chronic joint disease characterized by articular cartilage degeneration. OA usually manifests as joint pain, limited mobility, and joint effusion. Currently, the primary OA treatment is non-steroidal anti-inflammatory drugs (NSAIDs). Although they can alleviate the disease’s clinical symptoms and signs, the drugs have some side effects. Selenium nanoparticles (SeNPs) may be an alternative to relieve OA symptoms.Materials and Results: We confirmed the anti-inflammatory effect of selenium nanoparticles (SeNPs) in vitro and in vivo experiments for OA disease in this study. In vitro experiments, we found that SeNPs could significantly reduce the expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the major inflammatory factors, and had significant anti-inflammatory and anti-arthritic effects. SeNPs can inhibit reactive oxygen species (ROS) production and increased glutathione peroxidase (GPx) activity in interleukin-1beta (IL-1β)-stimulated cells. Additionally, SeNPs down-regulated matrix metalloproteinase-13 (MMP-13) and thrombospondin motifs 5 (ADAMTS-5) expressions, while up-regulated type II collagen (COL-2) and aggrecan (ACAN) expressions stimulated by IL-1β. The findings also indicated that SeNPs may exert their effects through suppressing the NF-κB p65 and p38/MAPK pathways. In vivo experiments, the prevention of OA development brought on by SeNPs was demonstrated using a DMM model.Discussion: Our results suggest that SeNPs may be a potential anti-inflammatory agent for treating OA.Keywords: osteoarthritis, NF-κB p65 signaling pathway, p38/MAPK signaling pathway, selenium nanoparticles |
| first_indexed | 2024-03-13T09:56:42Z |
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| language | English |
| last_indexed | 2024-03-13T09:56:42Z |
| publishDate | 2023-05-01 |
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| series | Drug Design, Development and Therapy |
| spelling | doaj.art-7a5391daf3004a5683ec8dd22221182c2023-05-23T18:52:21ZengDove Medical PressDrug Design, Development and Therapy1177-88812023-05-01Volume 171515152983883The Protective Effect of Selenium Nanoparticles in Osteoarthritis: In vitro and in vivo StudiesLi YZhu SLuo JTong YZheng YJi LHe ZJing QHuang JZhang YBi QYong Li,1,2,* Senbo Zhu,2,* Junchao Luo,2 Yu Tong,2 Yixuan Zheng,1 Lichen Ji,2 Zeju He,2 Qiangan Jing,1 Jiaqing Huang,2 Yinjun Zhang,1 Qing Bi2 1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, People’s Republic of China; 2Laboratory Medicine Center, Department of Laboratory Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, 310014, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yinjun Zhang, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, People’s Republic of China, Tel +86-13735494556, Email zhangyj@zjut.edu.cn Qing Bi, Laboratory Medicine Center, Department of Laboratory Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, 310014, People’s Republic of China, Tel +86-13588302991, Email bqzjsrmyy@163.comIntroduction: Osteoarthritis (OA) is a common chronic joint disease characterized by articular cartilage degeneration. OA usually manifests as joint pain, limited mobility, and joint effusion. Currently, the primary OA treatment is non-steroidal anti-inflammatory drugs (NSAIDs). Although they can alleviate the disease’s clinical symptoms and signs, the drugs have some side effects. Selenium nanoparticles (SeNPs) may be an alternative to relieve OA symptoms.Materials and Results: We confirmed the anti-inflammatory effect of selenium nanoparticles (SeNPs) in vitro and in vivo experiments for OA disease in this study. In vitro experiments, we found that SeNPs could significantly reduce the expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the major inflammatory factors, and had significant anti-inflammatory and anti-arthritic effects. SeNPs can inhibit reactive oxygen species (ROS) production and increased glutathione peroxidase (GPx) activity in interleukin-1beta (IL-1β)-stimulated cells. Additionally, SeNPs down-regulated matrix metalloproteinase-13 (MMP-13) and thrombospondin motifs 5 (ADAMTS-5) expressions, while up-regulated type II collagen (COL-2) and aggrecan (ACAN) expressions stimulated by IL-1β. The findings also indicated that SeNPs may exert their effects through suppressing the NF-κB p65 and p38/MAPK pathways. In vivo experiments, the prevention of OA development brought on by SeNPs was demonstrated using a DMM model.Discussion: Our results suggest that SeNPs may be a potential anti-inflammatory agent for treating OA.Keywords: osteoarthritis, NF-κB p65 signaling pathway, p38/MAPK signaling pathway, selenium nanoparticleshttps://www.dovepress.com/the-protective-effect-of-selenium-nanoparticles-in-osteoarthritis-in-v-peer-reviewed-fulltext-article-DDDTosteoarthritisnf-κb p65 signaling pathwayp38/mapk signaling pathwayselenium nanoparticles |
| spellingShingle | Li Y Zhu S Luo J Tong Y Zheng Y Ji L He Z Jing Q Huang J Zhang Y Bi Q The Protective Effect of Selenium Nanoparticles in Osteoarthritis: In vitro and in vivo Studies Drug Design, Development and Therapy osteoarthritis nf-κb p65 signaling pathway p38/mapk signaling pathway selenium nanoparticles |
| title | The Protective Effect of Selenium Nanoparticles in Osteoarthritis: In vitro and in vivo Studies |
| title_full | The Protective Effect of Selenium Nanoparticles in Osteoarthritis: In vitro and in vivo Studies |
| title_fullStr | The Protective Effect of Selenium Nanoparticles in Osteoarthritis: In vitro and in vivo Studies |
| title_full_unstemmed | The Protective Effect of Selenium Nanoparticles in Osteoarthritis: In vitro and in vivo Studies |
| title_short | The Protective Effect of Selenium Nanoparticles in Osteoarthritis: In vitro and in vivo Studies |
| title_sort | protective effect of selenium nanoparticles in osteoarthritis in vitro and in vivo studies |
| topic | osteoarthritis nf-κb p65 signaling pathway p38/mapk signaling pathway selenium nanoparticles |
| url | https://www.dovepress.com/the-protective-effect-of-selenium-nanoparticles-in-osteoarthritis-in-v-peer-reviewed-fulltext-article-DDDT |
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