Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis.
How and when potential becomes restricted in differentiating stem cell daughters is poorly understood. While it is thought that signals from the niche are actively required to prevent differentiation, another model proposes that stem cells can reversibly transit between multiple states, some of whic...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-12-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1009609 |
| _version_ | 1811252186765590528 |
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| author | Alice C Yuen Kenzo-Hugo Hillion Ruoxu Wang Marc Amoyel |
| author_facet | Alice C Yuen Kenzo-Hugo Hillion Ruoxu Wang Marc Amoyel |
| author_sort | Alice C Yuen |
| collection | DOAJ |
| description | How and when potential becomes restricted in differentiating stem cell daughters is poorly understood. While it is thought that signals from the niche are actively required to prevent differentiation, another model proposes that stem cells can reversibly transit between multiple states, some of which are primed, but not committed, to differentiate. In the Drosophila testis, somatic cyst stem cells (CySCs) generate cyst cells, which encapsulate the germline to support its development. We find that CySCs are maintained independently of niche self-renewal signals if activity of the PI3K/Tor pathway is inhibited. Conversely, PI3K/Tor is not sufficient alone to drive differentiation, suggesting that it acts to license cells for differentiation. Indeed, we find that the germline is required for differentiation of CySCs in response to PI3K/Tor elevation, indicating that final commitment to differentiation involves several steps and intercellular communication. We propose that CySC daughter cells are plastic, that their fate depends on the availability of neighbouring germ cells, and that PI3K/Tor acts to induce a primed state for CySC daughters to enable coordinated differentiation with the germline. |
| first_indexed | 2024-04-12T16:31:06Z |
| format | Article |
| id | doaj.art-7a556d5ac3b747268ae6c5c818ec432b |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-12T16:31:06Z |
| publishDate | 2021-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-7a556d5ac3b747268ae6c5c818ec432b2022-12-22T03:25:08ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-12-011712e100960910.1371/journal.pgen.1009609Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis.Alice C YuenKenzo-Hugo HillionRuoxu WangMarc AmoyelHow and when potential becomes restricted in differentiating stem cell daughters is poorly understood. While it is thought that signals from the niche are actively required to prevent differentiation, another model proposes that stem cells can reversibly transit between multiple states, some of which are primed, but not committed, to differentiate. In the Drosophila testis, somatic cyst stem cells (CySCs) generate cyst cells, which encapsulate the germline to support its development. We find that CySCs are maintained independently of niche self-renewal signals if activity of the PI3K/Tor pathway is inhibited. Conversely, PI3K/Tor is not sufficient alone to drive differentiation, suggesting that it acts to license cells for differentiation. Indeed, we find that the germline is required for differentiation of CySCs in response to PI3K/Tor elevation, indicating that final commitment to differentiation involves several steps and intercellular communication. We propose that CySC daughter cells are plastic, that their fate depends on the availability of neighbouring germ cells, and that PI3K/Tor acts to induce a primed state for CySC daughters to enable coordinated differentiation with the germline.https://doi.org/10.1371/journal.pgen.1009609 |
| spellingShingle | Alice C Yuen Kenzo-Hugo Hillion Ruoxu Wang Marc Amoyel Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis. PLoS Genetics |
| title | Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis. |
| title_full | Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis. |
| title_fullStr | Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis. |
| title_full_unstemmed | Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis. |
| title_short | Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis. |
| title_sort | germ cells commit somatic stem cells to differentiation following priming by pi3k tor activity in the drosophila testis |
| url | https://doi.org/10.1371/journal.pgen.1009609 |
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