Ammonia-oxidizing archaea and ammonia-oxidizing bacteria communities respond differently in oxy-gen-limited habitats
Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are the most important ammonia oxidation functional community, while the coastal environment just provides a different oxygen environment for the ammonia oxidation process. However, few surveys concentrated on the influence of oxygen concentration o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Environmental Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenvs.2022.976618/full |
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| author | Jialin Du Jialin Du Lin Meng Mingsheng Qiu Shuaiwei Chen Binghui Zhang Wenjing Song Ping Cong Xuebo Zheng |
| author_facet | Jialin Du Jialin Du Lin Meng Mingsheng Qiu Shuaiwei Chen Binghui Zhang Wenjing Song Ping Cong Xuebo Zheng |
| author_sort | Jialin Du |
| collection | DOAJ |
| description | Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are the most important ammonia oxidation functional community, while the coastal environment just provides a different oxygen environment for the ammonia oxidation process. However, few surveys concentrated on the influence of oxygen concentration on the niche specialization of AOA and AOB in the ocean intertidal zones. Here, high-throughput sequencing by Illumina MiSeq and qPCR were applied to detect the change of abundance, diversity as well as community structure of both AOA and AOB with 0–60 cm sediments depth in the intertidal zone in Qingdao, China. Results showed that the AOA/AOB amoA gene copy numbers and AOA/AOB OTU numbers rate increased as sediment depth went more profound, which indicated that AOA was more adaptive to oxygen-limited niches compared to AOB. Oxygen indeed led to the niche specialization of AOA and AOB in intertidal sediments. The dominant AOA and AOB were the clusters of Nitrosopumilus and Nitrosospira, respectively, which indicated ecological success in the intertidal zone. A significant and positive correlation (p < 0.01) between AOB abundance/AOB OTU numbers and Oxidation-reduction potential (ORP) was observed. In addition, both total nitrogen (TN) (p < 0.01) and pH (p < 0.05) were significantly negatively correlated to AOB abundance. TN was also significantly negatively correlated to AOB OTU numbers (p < 0.05). Hence, oxygen led to niche specialization of AOA and AOB, especially under anoxic conditions, AOA played a dominant role in the process of ammonia oxidation. The Nitrosopumilus and Nitrosospira clusters were the dominant AOA and AOB, respectively, representing an ecological success in the intertidal zone. |
| first_indexed | 2024-04-14T03:10:30Z |
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| institution | Directory Open Access Journal |
| issn | 2296-665X |
| language | English |
| last_indexed | 2024-04-14T03:10:30Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Environmental Science |
| spelling | doaj.art-7a577972aa6e43d6bd7ea0a335685b642022-12-22T02:15:37ZengFrontiers Media S.A.Frontiers in Environmental Science2296-665X2022-08-011010.3389/fenvs.2022.976618976618Ammonia-oxidizing archaea and ammonia-oxidizing bacteria communities respond differently in oxy-gen-limited habitatsJialin Du0Jialin Du1Lin Meng2Mingsheng Qiu3Shuaiwei Chen4Binghui Zhang5Wenjing Song6Ping Cong7Xuebo Zheng8Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaLongyan City Company of Fujian Tobacco Company, Longyan, ChinaChina Tobacco Shandong Industrial Co., Ltd., Longyan, ChinaFujian Branch of China Tobacco Company, Fuzhou, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaAmmonia-oxidizing archaea (AOA) and bacteria (AOB) are the most important ammonia oxidation functional community, while the coastal environment just provides a different oxygen environment for the ammonia oxidation process. However, few surveys concentrated on the influence of oxygen concentration on the niche specialization of AOA and AOB in the ocean intertidal zones. Here, high-throughput sequencing by Illumina MiSeq and qPCR were applied to detect the change of abundance, diversity as well as community structure of both AOA and AOB with 0–60 cm sediments depth in the intertidal zone in Qingdao, China. Results showed that the AOA/AOB amoA gene copy numbers and AOA/AOB OTU numbers rate increased as sediment depth went more profound, which indicated that AOA was more adaptive to oxygen-limited niches compared to AOB. Oxygen indeed led to the niche specialization of AOA and AOB in intertidal sediments. The dominant AOA and AOB were the clusters of Nitrosopumilus and Nitrosospira, respectively, which indicated ecological success in the intertidal zone. A significant and positive correlation (p < 0.01) between AOB abundance/AOB OTU numbers and Oxidation-reduction potential (ORP) was observed. In addition, both total nitrogen (TN) (p < 0.01) and pH (p < 0.05) were significantly negatively correlated to AOB abundance. TN was also significantly negatively correlated to AOB OTU numbers (p < 0.05). Hence, oxygen led to niche specialization of AOA and AOB, especially under anoxic conditions, AOA played a dominant role in the process of ammonia oxidation. The Nitrosopumilus and Nitrosospira clusters were the dominant AOA and AOB, respectively, representing an ecological success in the intertidal zone.https://www.frontiersin.org/articles/10.3389/fenvs.2022.976618/fullAOAAOBniche specializationintertidal zonehigh-throughput sequencing |
| spellingShingle | Jialin Du Jialin Du Lin Meng Mingsheng Qiu Shuaiwei Chen Binghui Zhang Wenjing Song Ping Cong Xuebo Zheng Ammonia-oxidizing archaea and ammonia-oxidizing bacteria communities respond differently in oxy-gen-limited habitats Frontiers in Environmental Science AOA AOB niche specialization intertidal zone high-throughput sequencing |
| title | Ammonia-oxidizing archaea and ammonia-oxidizing bacteria communities respond differently in oxy-gen-limited habitats |
| title_full | Ammonia-oxidizing archaea and ammonia-oxidizing bacteria communities respond differently in oxy-gen-limited habitats |
| title_fullStr | Ammonia-oxidizing archaea and ammonia-oxidizing bacteria communities respond differently in oxy-gen-limited habitats |
| title_full_unstemmed | Ammonia-oxidizing archaea and ammonia-oxidizing bacteria communities respond differently in oxy-gen-limited habitats |
| title_short | Ammonia-oxidizing archaea and ammonia-oxidizing bacteria communities respond differently in oxy-gen-limited habitats |
| title_sort | ammonia oxidizing archaea and ammonia oxidizing bacteria communities respond differently in oxy gen limited habitats |
| topic | AOA AOB niche specialization intertidal zone high-throughput sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fenvs.2022.976618/full |
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