CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification

High-throughput sequencing has allowed for unprecedented detail in gene expression analyses, yet its efficient application to single cells is challenged by the small starting amounts of RNA. We have developed CEL-Seq, a method for overcoming this limitation by barcoding and pooling samples before li...

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Bibliographic Details
Main Authors: Tamar Hashimshony, Florian Wagner, Noa Sher, Itai Yanai
Format: Article
Language:English
Published: Elsevier 2012-09-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124712002288