Fermentation of increasing ratios of grain starch and straw fiber: effects on hydrogen allocation and methanogenesis through in vitro ruminal batch culture
Grain starch has a faster rate of rumen fermentation than straw fiber and causes a rapid increase in ruminal molecular hydrogen (H2) partial pressure, which may promote other H2 sinks to compete H2 away from methanogenesis. The study was designed to investigate the effects of increasing ratios of gr...
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| Format: | Article |
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PeerJ Inc.
2023-04-01
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| Online Access: | https://peerj.com/articles/15050.pdf |
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| author | Siyu Yi Xiumin Zhang Xuezong Chen Juwang Zhou Cheng Gao Zhiyuan Ma Rong Wang Zhiliang Tan Min Wang |
| author_facet | Siyu Yi Xiumin Zhang Xuezong Chen Juwang Zhou Cheng Gao Zhiyuan Ma Rong Wang Zhiliang Tan Min Wang |
| author_sort | Siyu Yi |
| collection | DOAJ |
| description | Grain starch has a faster rate of rumen fermentation than straw fiber and causes a rapid increase in ruminal molecular hydrogen (H2) partial pressure, which may promote other H2 sinks to compete H2 away from methanogenesis. The study was designed to investigate the effects of increasing ratios of grain starch to straw fiber on hydrogen allocation and methanogenesis through in vitro ruminal batch incubation. Corn grain and corn straw were employed as starch and fiber source respectively. Seven treatments were the ratios of corn grain to corn straw (RGS) being 0:6, 1:5, 2:4, 3:3, 4:2, 5:1, and 6:0. Elevating RGS increased dry matter (DM) degradation and decreased methane (CH4) and hydrogen gas (gH2) production relative to DM degraded. Elevating RGS increased volatile fatty acid (VFA) concentration, propionate molar percentage and microbial protein (MCP) concentration, decreased acetate molar percentage, acetate to propionate ratio and estimated net metabolic hydrogen ([H]) production relative to DM degraded. Elevating RGS decreased the molar percentage of [H] utilized for CH4 and gH2 production. In summary, increasing ratios of grain starch to straw fiber altered rumen fermentation pathway from acetate to propionate production, reduced the efficiency of [H] production with the enhancement of MCP synthesis, and led to a reduction in the efficiency of CH4 and gH2 production. |
| first_indexed | 2024-03-09T06:52:09Z |
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| id | doaj.art-7a5cb1ec77b3427da11aace68b80fef3 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:52:09Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-7a5cb1ec77b3427da11aace68b80fef32023-12-03T10:24:10ZengPeerJ Inc.PeerJ2167-83592023-04-0111e1505010.7717/peerj.15050Fermentation of increasing ratios of grain starch and straw fiber: effects on hydrogen allocation and methanogenesis through in vitro ruminal batch cultureSiyu Yi0Xiumin Zhang1Xuezong Chen2Juwang Zhou3Cheng Gao4Zhiyuan Ma5Rong Wang6Zhiliang Tan7Min Wang8Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaKey Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, ChinaGrain starch has a faster rate of rumen fermentation than straw fiber and causes a rapid increase in ruminal molecular hydrogen (H2) partial pressure, which may promote other H2 sinks to compete H2 away from methanogenesis. The study was designed to investigate the effects of increasing ratios of grain starch to straw fiber on hydrogen allocation and methanogenesis through in vitro ruminal batch incubation. Corn grain and corn straw were employed as starch and fiber source respectively. Seven treatments were the ratios of corn grain to corn straw (RGS) being 0:6, 1:5, 2:4, 3:3, 4:2, 5:1, and 6:0. Elevating RGS increased dry matter (DM) degradation and decreased methane (CH4) and hydrogen gas (gH2) production relative to DM degraded. Elevating RGS increased volatile fatty acid (VFA) concentration, propionate molar percentage and microbial protein (MCP) concentration, decreased acetate molar percentage, acetate to propionate ratio and estimated net metabolic hydrogen ([H]) production relative to DM degraded. Elevating RGS decreased the molar percentage of [H] utilized for CH4 and gH2 production. In summary, increasing ratios of grain starch to straw fiber altered rumen fermentation pathway from acetate to propionate production, reduced the efficiency of [H] production with the enhancement of MCP synthesis, and led to a reduction in the efficiency of CH4 and gH2 production.https://peerj.com/articles/15050.pdfStarchFiberRumen fermentationHydrogenMethane |
| spellingShingle | Siyu Yi Xiumin Zhang Xuezong Chen Juwang Zhou Cheng Gao Zhiyuan Ma Rong Wang Zhiliang Tan Min Wang Fermentation of increasing ratios of grain starch and straw fiber: effects on hydrogen allocation and methanogenesis through in vitro ruminal batch culture PeerJ Starch Fiber Rumen fermentation Hydrogen Methane |
| title | Fermentation of increasing ratios of grain starch and straw fiber: effects on hydrogen allocation and methanogenesis through in vitro ruminal batch culture |
| title_full | Fermentation of increasing ratios of grain starch and straw fiber: effects on hydrogen allocation and methanogenesis through in vitro ruminal batch culture |
| title_fullStr | Fermentation of increasing ratios of grain starch and straw fiber: effects on hydrogen allocation and methanogenesis through in vitro ruminal batch culture |
| title_full_unstemmed | Fermentation of increasing ratios of grain starch and straw fiber: effects on hydrogen allocation and methanogenesis through in vitro ruminal batch culture |
| title_short | Fermentation of increasing ratios of grain starch and straw fiber: effects on hydrogen allocation and methanogenesis through in vitro ruminal batch culture |
| title_sort | fermentation of increasing ratios of grain starch and straw fiber effects on hydrogen allocation and methanogenesis through in vitro ruminal batch culture |
| topic | Starch Fiber Rumen fermentation Hydrogen Methane |
| url | https://peerj.com/articles/15050.pdf |
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