<b>Toxicity in food flavorings at the cellular level associated with each other at different doses
This study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2016-07-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475 |
| _version_ | 1811229238642081792 |
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| author | Sara Iolanda Oliveira da Silva Gleuvânia Marques Santana Ila Monize Sousa Sales João Marcelo de Castro e Sousa Ana Paula Peron |
| author_facet | Sara Iolanda Oliveira da Silva Gleuvânia Marques Santana Ila Monize Sousa Sales João Marcelo de Castro e Sousa Ana Paula Peron |
| author_sort | Sara Iolanda Oliveira da Silva |
| collection | DOAJ |
| description | This study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in combination, in which one of the three doses of a flavoring was combined with a different dose of one of the two other flavor additives studied. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and then analyzed, under light microscopy, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. All the treatments with combinations between the flavorings Chocolate/Strawberry and Strawberry/Condensed Milk reduced, in both exposure times considered, cell division of A. cepa roots, proving to be cytotoxic. In turn, the treatments with the association of Chocolate/Condensed Milk did not change significantly the mitotic index of the cells analyzed. The Strawberry flavoring was the most cytotoxic among the additives tested. None of the evaluated associations was genotoxic under the study conditions.
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| first_indexed | 2024-04-12T10:11:12Z |
| format | Article |
| id | doaj.art-7a5d3a1d84624498b4ccb45f68bdb684 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-12T10:11:12Z |
| publishDate | 2016-07-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-7a5d3a1d84624498b4ccb45f68bdb6842022-12-22T03:37:19ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2016-07-0138110.4025/actascibiolsci.v38i1.3047513558<b>Toxicity in food flavorings at the cellular level associated with each other at different dosesSara Iolanda Oliveira da Silva0Gleuvânia Marques Santana1Ila Monize Sousa Sales2João Marcelo de Castro e Sousa3Ana Paula PeronUniversidade Federal do PiauíUniversidade Federal do PiauíUniversidade Federal do PiauíUniversidade Federal do PiauíThis study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in combination, in which one of the three doses of a flavoring was combined with a different dose of one of the two other flavor additives studied. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and then analyzed, under light microscopy, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. All the treatments with combinations between the flavorings Chocolate/Strawberry and Strawberry/Condensed Milk reduced, in both exposure times considered, cell division of A. cepa roots, proving to be cytotoxic. In turn, the treatments with the association of Chocolate/Condensed Milk did not change significantly the mitotic index of the cells analyzed. The Strawberry flavoring was the most cytotoxic among the additives tested. None of the evaluated associations was genotoxic under the study conditions. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475aroma and flavor additivescytotoxicitygenotoxicityA. cepa |
| spellingShingle | Sara Iolanda Oliveira da Silva Gleuvânia Marques Santana Ila Monize Sousa Sales João Marcelo de Castro e Sousa Ana Paula Peron <b>Toxicity in food flavorings at the cellular level associated with each other at different doses Acta Scientiarum: Biological Sciences aroma and flavor additives cytotoxicity genotoxicity A. cepa |
| title | <b>Toxicity in food flavorings at the cellular level associated with each other at different doses |
| title_full | <b>Toxicity in food flavorings at the cellular level associated with each other at different doses |
| title_fullStr | <b>Toxicity in food flavorings at the cellular level associated with each other at different doses |
| title_full_unstemmed | <b>Toxicity in food flavorings at the cellular level associated with each other at different doses |
| title_short | <b>Toxicity in food flavorings at the cellular level associated with each other at different doses |
| title_sort | b toxicity in food flavorings at the cellular level associated with each other at different doses |
| topic | aroma and flavor additives cytotoxicity genotoxicity A. cepa |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475 |
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