| Summary: | Grapes and apples are the most cultivated fruits in the Mediterranean basin and their agricultural processing is responsible for the production of a large amount of bio-waste. The reuse of this food biomass would increase the volume of recyclable/renewable biomaterial and lower the environmental impact due to the increasing demand for these biological products. To this purpose, agri-food waste from grape and apple processing have become an important source of phytochemicals, and many pharmaceutical industries are using it as starting material to produce dietary supplements, functional foods, and food additives for human consumption. In virtue of the chemical diversity and complexity of agri-food biowaste, developers and producers of nutraceuticals are advised to assess the safety of their final nutraceutical products, in compliance with European Food Safety Authority regulation. Here, we use the Ames test to assess the mutagenicity of three nutraceuticals obtained from agri-food waste biomasses: Taurisolo<sup>®</sup> from grape pomace of <i>Vitis vinifera</i> L. cv ‘Aglianico’, AnnurComplex<sup>®</sup> from <i>Malus pumila</i> M. cv ‘Annurca’ and Limoncella Apple Extract from <i>Malus domestica</i> B. cv ‘Limoncella’. The results showed that all three nutraceuticals were non-mutagenic.
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