Effects of Tylosin, a Direct-Fed Microbial and Feedlot Pen Environment on Phenotypic Resistance among Enterococci Isolated from Beef Cattle Feces
In two sequential replicates (<i>n</i> = 90 and <i>n</i> = 96 feedlot finisher cattle, respectively) we measured the impact of an <i>Enterococcus faecium</i>-based probiotic (DFM) and an altered feedlot pen environment on antimicrobial resistance among fecal enter...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/1/106 |
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| author | Sarah A. Murray Ashlyn C. Holbert Keri N. Norman Sara D. Lawhon Jason E. Sawyer Harvey M. Scott |
| author_facet | Sarah A. Murray Ashlyn C. Holbert Keri N. Norman Sara D. Lawhon Jason E. Sawyer Harvey M. Scott |
| author_sort | Sarah A. Murray |
| collection | DOAJ |
| description | In two sequential replicates (<i>n</i> = 90 and <i>n</i> = 96 feedlot finisher cattle, respectively) we measured the impact of an <i>Enterococcus faecium</i>-based probiotic (DFM) and an altered feedlot pen environment on antimicrobial resistance among fecal enterococci in cattle fed (or, not fed) the macrolide tylosin. Diluted fecal samples were spiral-plated on plain and antibiotic-supplemented m-<i>Enterococcus</i> agar. In the first replicate, tylosin significantly (<i>p</i> < 0.05) increased the relative quantity of erythromycin-resistant enterococci. This effect was diminished in cattle fed the DFM in conjunction with tylosin, indicating a macrolide susceptible probiotic may help mitigate resistance. A similar observed effect was not statistically significant (<i>p</i> > 0.05) in the second replicate. Isolates were speciated and resistance phenotypes were obtained for <i>E. faecium</i> and <i>E. hirae</i>. Susceptible strains of bacteria fed as DFM may prove useful for mitigating the selective effects of antibiotic use; however, the longer-term sustainability of such an approach remains unclear. |
| first_indexed | 2024-03-10T03:01:48Z |
| format | Article |
| id | doaj.art-7a5f77af3a804c71a8f1fc0504e97964 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:01:48Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-7a5f77af3a804c71a8f1fc0504e979642023-11-23T12:45:28ZengMDPI AGAntibiotics2079-63822022-01-0111110610.3390/antibiotics11010106Effects of Tylosin, a Direct-Fed Microbial and Feedlot Pen Environment on Phenotypic Resistance among Enterococci Isolated from Beef Cattle FecesSarah A. Murray0Ashlyn C. Holbert1Keri N. Norman2Sara D. Lawhon3Jason E. Sawyer4Harvey M. Scott5Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USADepartment of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USADepartment of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USADepartment of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USADepartment of Animal Science, Texas A&M University, College Station, TX 77843, USADepartment of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USAIn two sequential replicates (<i>n</i> = 90 and <i>n</i> = 96 feedlot finisher cattle, respectively) we measured the impact of an <i>Enterococcus faecium</i>-based probiotic (DFM) and an altered feedlot pen environment on antimicrobial resistance among fecal enterococci in cattle fed (or, not fed) the macrolide tylosin. Diluted fecal samples were spiral-plated on plain and antibiotic-supplemented m-<i>Enterococcus</i> agar. In the first replicate, tylosin significantly (<i>p</i> < 0.05) increased the relative quantity of erythromycin-resistant enterococci. This effect was diminished in cattle fed the DFM in conjunction with tylosin, indicating a macrolide susceptible probiotic may help mitigate resistance. A similar observed effect was not statistically significant (<i>p</i> > 0.05) in the second replicate. Isolates were speciated and resistance phenotypes were obtained for <i>E. faecium</i> and <i>E. hirae</i>. Susceptible strains of bacteria fed as DFM may prove useful for mitigating the selective effects of antibiotic use; however, the longer-term sustainability of such an approach remains unclear.https://www.mdpi.com/2079-6382/11/1/106probiotic<i>Enterococcus faecium</i>antimicrobial resistanceenvironmental change |
| spellingShingle | Sarah A. Murray Ashlyn C. Holbert Keri N. Norman Sara D. Lawhon Jason E. Sawyer Harvey M. Scott Effects of Tylosin, a Direct-Fed Microbial and Feedlot Pen Environment on Phenotypic Resistance among Enterococci Isolated from Beef Cattle Feces Antibiotics probiotic <i>Enterococcus faecium</i> antimicrobial resistance environmental change |
| title | Effects of Tylosin, a Direct-Fed Microbial and Feedlot Pen Environment on Phenotypic Resistance among Enterococci Isolated from Beef Cattle Feces |
| title_full | Effects of Tylosin, a Direct-Fed Microbial and Feedlot Pen Environment on Phenotypic Resistance among Enterococci Isolated from Beef Cattle Feces |
| title_fullStr | Effects of Tylosin, a Direct-Fed Microbial and Feedlot Pen Environment on Phenotypic Resistance among Enterococci Isolated from Beef Cattle Feces |
| title_full_unstemmed | Effects of Tylosin, a Direct-Fed Microbial and Feedlot Pen Environment on Phenotypic Resistance among Enterococci Isolated from Beef Cattle Feces |
| title_short | Effects of Tylosin, a Direct-Fed Microbial and Feedlot Pen Environment on Phenotypic Resistance among Enterococci Isolated from Beef Cattle Feces |
| title_sort | effects of tylosin a direct fed microbial and feedlot pen environment on phenotypic resistance among enterococci isolated from beef cattle feces |
| topic | probiotic <i>Enterococcus faecium</i> antimicrobial resistance environmental change |
| url | https://www.mdpi.com/2079-6382/11/1/106 |
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