Assembly of Endophytic Communities of Setaria viridis Plants when Grown in Different Soils and Derived from Different Seeds
The association of the plant microbiota is a successional process that starts with the seed and its intrinsic microbiota. The recently reported relevance of seeds as carriers of microbiota has encouraged investigations of the assembly of these communities in different tissues. Here, we address the c...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2024-03-01
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| Series: | Phytobiomes Journal |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-08-22-0054-MF |
| _version_ | 1797278213782634496 |
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| author | Carolina Escobar Rodríguez Livio Antonielli Birgit Mitter Angela Sessitsch |
| author_facet | Carolina Escobar Rodríguez Livio Antonielli Birgit Mitter Angela Sessitsch |
| author_sort | Carolina Escobar Rodríguez |
| collection | DOAJ |
| description | The association of the plant microbiota is a successional process that starts with the seed and its intrinsic microbiota. The recently reported relevance of seeds as carriers of microbiota has encouraged investigations of the assembly of these communities in different tissues. Here, we address the contributions of both seed and soil bacterial microbiota in the assembly of communities within endospheres of adult plants by 16S ribosomal RNA gene-based Illumina sequencing. Surface-sterilized seeds of the model plant Setaria viridis were sown in sterile conditions and seedlings were transferred onto either their native soil or a foreign soil. Soil-derived bacterial colonizers contributed to the highest portion of endophytic microbiota, with varying community composition depending on the cultivation soil. In contrast, the contribution of seed microbiota in those of adult plants was less evident. Moreover, seedlings grown in their native soils resulted in plants with consistent endophytic assemblages, whereas a dramatic increase in variability was observed for rhizosphere and endophytic root communities of plants grown in foreign soils. |
| first_indexed | 2024-03-07T16:00:05Z |
| format | Article |
| id | doaj.art-7a612d4743ad40a8957e22fa71d428b2 |
| institution | Directory Open Access Journal |
| issn | 2471-2906 |
| language | English |
| last_indexed | 2024-03-07T16:00:05Z |
| publishDate | 2024-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Phytobiomes Journal |
| spelling | doaj.art-7a612d4743ad40a8957e22fa71d428b22024-03-04T21:39:14ZengThe American Phytopathological SocietyPhytobiomes Journal2471-29062024-03-0181344510.1094/PBIOMES-08-22-0054-MFAssembly of Endophytic Communities of Setaria viridis Plants when Grown in Different Soils and Derived from Different SeedsCarolina Escobar Rodríguez0Livio Antonielli1Birgit Mitter2Angela Sessitsch3Center for Health & Bioresources, Bioresources Unit, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Strasse 24, 3430 Tulln, AustriaCenter for Health & Bioresources, Bioresources Unit, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Strasse 24, 3430 Tulln, AustriaCenter for Health & Bioresources, Bioresources Unit, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Strasse 24, 3430 Tulln, AustriaCenter for Health & Bioresources, Bioresources Unit, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Strasse 24, 3430 Tulln, AustriaThe association of the plant microbiota is a successional process that starts with the seed and its intrinsic microbiota. The recently reported relevance of seeds as carriers of microbiota has encouraged investigations of the assembly of these communities in different tissues. Here, we address the contributions of both seed and soil bacterial microbiota in the assembly of communities within endospheres of adult plants by 16S ribosomal RNA gene-based Illumina sequencing. Surface-sterilized seeds of the model plant Setaria viridis were sown in sterile conditions and seedlings were transferred onto either their native soil or a foreign soil. Soil-derived bacterial colonizers contributed to the highest portion of endophytic microbiota, with varying community composition depending on the cultivation soil. In contrast, the contribution of seed microbiota in those of adult plants was less evident. Moreover, seedlings grown in their native soils resulted in plants with consistent endophytic assemblages, whereas a dramatic increase in variability was observed for rhizosphere and endophytic root communities of plants grown in foreign soils.https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-08-22-0054-MFendophytic microbiotamodel grassseed bacterial microbiotaSetaria microbiota |
| spellingShingle | Carolina Escobar Rodríguez Livio Antonielli Birgit Mitter Angela Sessitsch Assembly of Endophytic Communities of Setaria viridis Plants when Grown in Different Soils and Derived from Different Seeds Phytobiomes Journal endophytic microbiota model grass seed bacterial microbiota Setaria microbiota |
| title | Assembly of Endophytic Communities of Setaria viridis Plants when Grown in Different Soils and Derived from Different Seeds |
| title_full | Assembly of Endophytic Communities of Setaria viridis Plants when Grown in Different Soils and Derived from Different Seeds |
| title_fullStr | Assembly of Endophytic Communities of Setaria viridis Plants when Grown in Different Soils and Derived from Different Seeds |
| title_full_unstemmed | Assembly of Endophytic Communities of Setaria viridis Plants when Grown in Different Soils and Derived from Different Seeds |
| title_short | Assembly of Endophytic Communities of Setaria viridis Plants when Grown in Different Soils and Derived from Different Seeds |
| title_sort | assembly of endophytic communities of setaria viridis plants when grown in different soils and derived from different seeds |
| topic | endophytic microbiota model grass seed bacterial microbiota Setaria microbiota |
| url | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-08-22-0054-MF |
| work_keys_str_mv | AT carolinaescobarrodriguez assemblyofendophyticcommunitiesofsetariaviridisplantswhengrownindifferentsoilsandderivedfromdifferentseeds AT livioantonielli assemblyofendophyticcommunitiesofsetariaviridisplantswhengrownindifferentsoilsandderivedfromdifferentseeds AT birgitmitter assemblyofendophyticcommunitiesofsetariaviridisplantswhengrownindifferentsoilsandderivedfromdifferentseeds AT angelasessitsch assemblyofendophyticcommunitiesofsetariaviridisplantswhengrownindifferentsoilsandderivedfromdifferentseeds |