Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration
Objective: Electrospun nanofibers exhibit potential as scaffolds for articular cartilage tissue regeneration. This study aimed to fabricate electrospun polycaprolactone (PCL)/silk fibroin (SF) composite nanofiber scaffolds and to explore performance of the scaffolds for articular chondrocyte regener...
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Materials |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmats.2023.1292098/full |
| _version_ | 1827219616393330688 |
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| author | Bowen Xie Bowen Xie Fengyuan Yang Fengyuan Yang Hongguang Chen Hongxing Zhang Hongxing Zhang Hebin Ma Tianqi Li Tianqi Li Zhiqiang Chen Zhiqiang Chen Jingyuan Li Jingyuan Li Xiaojie Li Junjie Du Junjie Du Junjie Du |
| author_facet | Bowen Xie Bowen Xie Fengyuan Yang Fengyuan Yang Hongguang Chen Hongxing Zhang Hongxing Zhang Hebin Ma Tianqi Li Tianqi Li Zhiqiang Chen Zhiqiang Chen Jingyuan Li Jingyuan Li Xiaojie Li Junjie Du Junjie Du Junjie Du |
| author_sort | Bowen Xie |
| collection | DOAJ |
| description | Objective: Electrospun nanofibers exhibit potential as scaffolds for articular cartilage tissue regeneration. This study aimed to fabricate electrospun polycaprolactone (PCL)/silk fibroin (SF) composite nanofiber scaffolds and to explore performance of the scaffolds for articular chondrocyte regeneration.Methods: By altering material composition and preparation methods, three types of nanofiber scaffolds were effectively fabricated, including randomly oriented PCL (RPCL) nanofiber scaffold, randomly oriented PCL/SF (RPCL/SF) nanofiber scaffold, and aligned PCL/SF (APCL/SF) nanofiber scaffold. Physiochemical analyses were performed to determine mechanical properties and surface hydrophilicity of the nanofiber scaffolds. In vitro studies were conducted to investigate performance of the scaffolds on articular chondrocyte proliferation, gene expression and glycosaminoglycan secretion. Cytoskeleton staining was used to observe the arrangement of chondrocytes along the direction of the fibers and their elongation along the fiber arrangement.Results: The physicochemical analysis demonstrated that the APCL/SF nanofiber scaffold exhibited improved mechanical properties and surface hydrophilicity compared to the RPCL and RPCL/SF nanofiber scaffolds. Furthermore, the in vitro cell culture studies confirmed that the APCL/SF nanofibers could significantly promote articular chondrocyte proliferation, type II collagen (COL-II) gene expression, and glycosaminoglycan secretion compared to the RPCL and RPCL/SF nanofiber scaffolds. Additionally, cytoskeletal staining displayed that the APCL/SF nanofiber scaffold promoted the elongation of articular chondrocytes in the direction of parallel fiber alignment.Conclusion: The APCL/SF nanofiber scaffold exhibited promising potential as a composite scaffold for articular cartilage regeneration. |
| first_indexed | 2024-03-11T13:32:31Z |
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| id | doaj.art-7a6190456021427da2a3f93e093e05e3 |
| institution | Directory Open Access Journal |
| issn | 2296-8016 |
| language | English |
| last_indexed | 2025-03-21T15:53:38Z |
| publishDate | 2023-11-01 |
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| series | Frontiers in Materials |
| spelling | doaj.art-7a6190456021427da2a3f93e093e05e32024-06-18T08:24:34ZengFrontiers Media S.A.Frontiers in Materials2296-80162023-11-011010.3389/fmats.2023.12920981292098Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regenerationBowen Xie0Bowen Xie1Fengyuan Yang2Fengyuan Yang3Hongguang Chen4Hongxing Zhang5Hongxing Zhang6Hebin Ma7Tianqi Li8Tianqi Li9Zhiqiang Chen10Zhiqiang Chen11Jingyuan Li12Jingyuan Li13Xiaojie Li14Junjie Du15Junjie Du16Junjie Du17Fifth School of Clinical Medicine, Air Force Clinical College, Anhui Medical University, Hefei, ChinaDepartment of Orthopedics, Air Force Medical Center of the PLA, Beijing, ChinaFifth School of Clinical Medicine, Air Force Clinical College, Anhui Medical University, Hefei, ChinaGraduate School of Medicine, China Medical University, Shenyang, ChinaSenior Department of Orthopedics, The Fourth Medical Center of the PLA General Hospital, Beijing, ChinaFifth School of Clinical Medicine, Air Force Clinical College, Anhui Medical University, Hefei, ChinaDepartment of Orthopedics, Air Force Medical Center of the PLA, Beijing, ChinaSenior Department of Orthopedics, The Fourth Medical Center of the PLA General Hospital, Beijing, ChinaFifth School of Clinical Medicine, Air Force Clinical College, Anhui Medical University, Hefei, ChinaGraduate School of Medicine, China Medical University, Shenyang, ChinaFifth School of Clinical Medicine, Air Force Clinical College, Anhui Medical University, Hefei, ChinaGraduate School of Medicine, China Medical University, Shenyang, ChinaFifth School of Clinical Medicine, Air Force Clinical College, Anhui Medical University, Hefei, ChinaDepartment of Orthopedics, Air Force Medical Center of the PLA, Beijing, ChinaFifth School of Clinical Medicine, Air Force Clinical College, Anhui Medical University, Hefei, ChinaFifth School of Clinical Medicine, Air Force Clinical College, Anhui Medical University, Hefei, ChinaDepartment of Orthopedics, Air Force Medical Center of the PLA, Beijing, ChinaGraduate School of Medicine, China Medical University, Shenyang, ChinaObjective: Electrospun nanofibers exhibit potential as scaffolds for articular cartilage tissue regeneration. This study aimed to fabricate electrospun polycaprolactone (PCL)/silk fibroin (SF) composite nanofiber scaffolds and to explore performance of the scaffolds for articular chondrocyte regeneration.Methods: By altering material composition and preparation methods, three types of nanofiber scaffolds were effectively fabricated, including randomly oriented PCL (RPCL) nanofiber scaffold, randomly oriented PCL/SF (RPCL/SF) nanofiber scaffold, and aligned PCL/SF (APCL/SF) nanofiber scaffold. Physiochemical analyses were performed to determine mechanical properties and surface hydrophilicity of the nanofiber scaffolds. In vitro studies were conducted to investigate performance of the scaffolds on articular chondrocyte proliferation, gene expression and glycosaminoglycan secretion. Cytoskeleton staining was used to observe the arrangement of chondrocytes along the direction of the fibers and their elongation along the fiber arrangement.Results: The physicochemical analysis demonstrated that the APCL/SF nanofiber scaffold exhibited improved mechanical properties and surface hydrophilicity compared to the RPCL and RPCL/SF nanofiber scaffolds. Furthermore, the in vitro cell culture studies confirmed that the APCL/SF nanofibers could significantly promote articular chondrocyte proliferation, type II collagen (COL-II) gene expression, and glycosaminoglycan secretion compared to the RPCL and RPCL/SF nanofiber scaffolds. Additionally, cytoskeletal staining displayed that the APCL/SF nanofiber scaffold promoted the elongation of articular chondrocytes in the direction of parallel fiber alignment.Conclusion: The APCL/SF nanofiber scaffold exhibited promising potential as a composite scaffold for articular cartilage regeneration.https://www.frontiersin.org/articles/10.3389/fmats.2023.1292098/fullpolycaprolactonesilk fibroinelectrospinningsurface morphologymaterial orientationarticular chondrocyte |
| spellingShingle | Bowen Xie Bowen Xie Fengyuan Yang Fengyuan Yang Hongguang Chen Hongxing Zhang Hongxing Zhang Hebin Ma Tianqi Li Tianqi Li Zhiqiang Chen Zhiqiang Chen Jingyuan Li Jingyuan Li Xiaojie Li Junjie Du Junjie Du Junjie Du Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration Frontiers in Materials polycaprolactone silk fibroin electrospinning surface morphology material orientation articular chondrocyte |
| title | Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration |
| title_full | Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration |
| title_fullStr | Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration |
| title_full_unstemmed | Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration |
| title_short | Electrospun polycaprolactone/silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration |
| title_sort | electrospun polycaprolactone silk fibroin nanofiber scaffold with aligned fiber orientation for articular chondrocyte regeneration |
| topic | polycaprolactone silk fibroin electrospinning surface morphology material orientation articular chondrocyte |
| url | https://www.frontiersin.org/articles/10.3389/fmats.2023.1292098/full |
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