Improving the solubility, activity, and stability of reteplase using in silico design of new variants
Reteplase (recombinant plasminogen activator, r-PA) is a thrombolytic agent recombined from tissue-type plasminogen activator (t-PA), which has several prominent features such as strong thrombolytic ability and E. coli expressibility. Despite these outstanding features, it demonstrates reduced fibri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Research in Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=4;spage=359;epage=368;aulast=Seyedhosseini |
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| author | Hooria Seyedhosseini Ghaheh Mohamad Reza Ganjalikhany Parichehreh Yaghmaei Morteza Pourfarzam Hamid Mir Mohammad Sadeghi |
| author_facet | Hooria Seyedhosseini Ghaheh Mohamad Reza Ganjalikhany Parichehreh Yaghmaei Morteza Pourfarzam Hamid Mir Mohammad Sadeghi |
| author_sort | Hooria Seyedhosseini Ghaheh |
| collection | DOAJ |
| description | Reteplase (recombinant plasminogen activator, r-PA) is a thrombolytic agent recombined from tissue-type plasminogen activator (t-PA), which has several prominent features such as strong thrombolytic ability and E. coli expressibility. Despite these outstanding features, it demonstrates reduced fibrin binding affinity, reduced stimulation of protease activity, and lower solubility, hence higher aggregation propensity, compared to t-PA. The present study was devoted to design r-PA variants with comparable structural stability, enhanced biological activity, and high solubility. For this purpose, computational molecular modeling techniques were utilized. The supercharging technique was applied for r-PA to designing new species of the protein. Based on the results from in silico evaluation of selected mutations in comparison to the wild-type r-PA, the designed supercharged mutant (S7 variant) exhibited augmented stability, decreased solvation energy, as well as enhanced binding affinity to fibrin. The data also implied increased plasminogen cleavage activity of the new variant. These findings have implications to therapies which involve removal of intravascular blood clots, including the treatment of acute myocardial infarction. |
| first_indexed | 2024-12-21T20:16:29Z |
| format | Article |
| id | doaj.art-7a62a8ed486941d5ad113c6eb85c555c |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-12-21T20:16:29Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-7a62a8ed486941d5ad113c6eb85c555c2022-12-21T18:51:35ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142019-01-0114435936810.4103/1735-5362.263560Improving the solubility, activity, and stability of reteplase using in silico design of new variantsHooria Seyedhosseini GhahehMohamad Reza GanjalikhanyParichehreh YaghmaeiMorteza PourfarzamHamid Mir Mohammad SadeghiReteplase (recombinant plasminogen activator, r-PA) is a thrombolytic agent recombined from tissue-type plasminogen activator (t-PA), which has several prominent features such as strong thrombolytic ability and E. coli expressibility. Despite these outstanding features, it demonstrates reduced fibrin binding affinity, reduced stimulation of protease activity, and lower solubility, hence higher aggregation propensity, compared to t-PA. The present study was devoted to design r-PA variants with comparable structural stability, enhanced biological activity, and high solubility. For this purpose, computational molecular modeling techniques were utilized. The supercharging technique was applied for r-PA to designing new species of the protein. Based on the results from in silico evaluation of selected mutations in comparison to the wild-type r-PA, the designed supercharged mutant (S7 variant) exhibited augmented stability, decreased solvation energy, as well as enhanced binding affinity to fibrin. The data also implied increased plasminogen cleavage activity of the new variant. These findings have implications to therapies which involve removal of intravascular blood clots, including the treatment of acute myocardial infarction.http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=4;spage=359;epage=368;aulast=Seyedhosseiniin silico design; reteplase; supercharging; thrombolysis; tissue-type plasminogen activator |
| spellingShingle | Hooria Seyedhosseini Ghaheh Mohamad Reza Ganjalikhany Parichehreh Yaghmaei Morteza Pourfarzam Hamid Mir Mohammad Sadeghi Improving the solubility, activity, and stability of reteplase using in silico design of new variants Research in Pharmaceutical Sciences in silico design; reteplase; supercharging; thrombolysis; tissue-type plasminogen activator |
| title | Improving the solubility, activity, and stability of reteplase using in silico design of new variants |
| title_full | Improving the solubility, activity, and stability of reteplase using in silico design of new variants |
| title_fullStr | Improving the solubility, activity, and stability of reteplase using in silico design of new variants |
| title_full_unstemmed | Improving the solubility, activity, and stability of reteplase using in silico design of new variants |
| title_short | Improving the solubility, activity, and stability of reteplase using in silico design of new variants |
| title_sort | improving the solubility activity and stability of reteplase using in silico design of new variants |
| topic | in silico design; reteplase; supercharging; thrombolysis; tissue-type plasminogen activator |
| url | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=4;spage=359;epage=368;aulast=Seyedhosseini |
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