Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring
Introduction : Maternal hypoxia induces an adverse uterine environment and may induce long-term effects in offspring. This study investigated whether maternal hypoxia increases hippocampal cell vulnerability and exacerbates neurological impairments in adult rat offspring following ischemia. Materi...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2017-12-01
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| Series: | Folia Neuropathologica |
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| Online Access: | https://www.termedia.pl/Maternal-hypoxia-increases-hippocampal-cell-susceptibility-to-ischemia-after-middle-cerebral-artery-occlusion-in-rat-offspring,20,31352,1,1.html |
| _version_ | 1819128141146226688 |
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| author | Lingxing Wang Honghong Huang Hongchao Cai Yafang Chen |
| author_facet | Lingxing Wang Honghong Huang Hongchao Cai Yafang Chen |
| author_sort | Lingxing Wang |
| collection | DOAJ |
| description | Introduction : Maternal hypoxia induces an adverse uterine environment and may induce long-term effects in offspring. This study investigated whether maternal hypoxia increases hippocampal cell vulnerability and exacerbates neurological impairments in adult rat offspring following ischemia.
Material and methods : Pregnant Sprague-Dawley rats were randomly assigned to no maternal hypoxia or maternal hypoxia treatment groups. Adult male rat offspring were subjected to middle cerebral artery occlusion (MCAO). There were four groups: maternal + sham (MH + Sham), sham (Sham), maternal hypoxia + MCAO (MH + MCAO), and MCAO only (MCAO). Neurological deficits were evaluated. Hippocampal cell damage was observed by hematoxylin and eosin (HE) staining. Cell apoptosis in the hippocampus was detected by TdT-mediated dUTP-biotin nick-end labeling (TUNEL) staining. Caspase-3, cytochrome c, Bax, and bcl-2 expression in the hippocampus was detected by Western blot.
Results : More severe hippocampal cell damage was found in the MH + MCAO group than in the MCAO group. Additionally, neurological deficits, percentage of TUNEL positive cells, and expression of caspase-3, cytochrome c, and Bax in the hippocampus were significantly higher (p < 0.05), whereas bcl-2 expression was significantly lower (p < 0.05) in the MH + MCAO group compared to the MCAO group.
Conclusions : These findings suggest that maternal hypoxia may exacerbate hippocampal cell apoptosis in rat offspring after MCAO via alterations in the expression of cytochrome c, caspase-3, Bax, and bcl-2, which ultimately affects ischemic stroke prognosis. To our knowledge, this is the first study demonstrating that maternal hypoxia increases hippocampal cell susceptibility to ischemia in adult rat offspring. |
| first_indexed | 2024-12-22T08:23:06Z |
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| id | doaj.art-7a62abeb097c47d6a2e34a1266e8c890 |
| institution | Directory Open Access Journal |
| issn | 1641-4640 1509-572X |
| language | English |
| last_indexed | 2024-12-22T08:23:06Z |
| publishDate | 2017-12-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Folia Neuropathologica |
| spelling | doaj.art-7a62abeb097c47d6a2e34a1266e8c8902022-12-21T18:32:42ZengTermedia Publishing HouseFolia Neuropathologica1641-46401509-572X2017-12-0155431732410.5114/fn.2017.7239231352Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspringLingxing WangHonghong HuangHongchao CaiYafang ChenIntroduction : Maternal hypoxia induces an adverse uterine environment and may induce long-term effects in offspring. This study investigated whether maternal hypoxia increases hippocampal cell vulnerability and exacerbates neurological impairments in adult rat offspring following ischemia. Material and methods : Pregnant Sprague-Dawley rats were randomly assigned to no maternal hypoxia or maternal hypoxia treatment groups. Adult male rat offspring were subjected to middle cerebral artery occlusion (MCAO). There were four groups: maternal + sham (MH + Sham), sham (Sham), maternal hypoxia + MCAO (MH + MCAO), and MCAO only (MCAO). Neurological deficits were evaluated. Hippocampal cell damage was observed by hematoxylin and eosin (HE) staining. Cell apoptosis in the hippocampus was detected by TdT-mediated dUTP-biotin nick-end labeling (TUNEL) staining. Caspase-3, cytochrome c, Bax, and bcl-2 expression in the hippocampus was detected by Western blot. Results : More severe hippocampal cell damage was found in the MH + MCAO group than in the MCAO group. Additionally, neurological deficits, percentage of TUNEL positive cells, and expression of caspase-3, cytochrome c, and Bax in the hippocampus were significantly higher (p < 0.05), whereas bcl-2 expression was significantly lower (p < 0.05) in the MH + MCAO group compared to the MCAO group. Conclusions : These findings suggest that maternal hypoxia may exacerbate hippocampal cell apoptosis in rat offspring after MCAO via alterations in the expression of cytochrome c, caspase-3, Bax, and bcl-2, which ultimately affects ischemic stroke prognosis. To our knowledge, this is the first study demonstrating that maternal hypoxia increases hippocampal cell susceptibility to ischemia in adult rat offspring.https://www.termedia.pl/Maternal-hypoxia-increases-hippocampal-cell-susceptibility-to-ischemia-after-middle-cerebral-artery-occlusion-in-rat-offspring,20,31352,1,1.html<I>maternal hypoxia offspring cerebral ischemia hippocampus disease susceptibility</I> |
| spellingShingle | Lingxing Wang Honghong Huang Hongchao Cai Yafang Chen Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring Folia Neuropathologica <I>maternal hypoxia offspring cerebral ischemia hippocampus disease susceptibility</I> |
| title | Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring |
| title_full | Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring |
| title_fullStr | Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring |
| title_full_unstemmed | Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring |
| title_short | Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring |
| title_sort | maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring |
| topic | <I>maternal hypoxia offspring cerebral ischemia hippocampus disease susceptibility</I> |
| url | https://www.termedia.pl/Maternal-hypoxia-increases-hippocampal-cell-susceptibility-to-ischemia-after-middle-cerebral-artery-occlusion-in-rat-offspring,20,31352,1,1.html |
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